Protein Variants | Comment | Organism |
---|---|---|
ALDH-H3tail | aldehyde dehydrogenase class 1 with the addition of the C-terminal tail of class 3, KM-value for propionaldehyde is 2.6fold higher than the KM-value of the wild-type enzyme, KM-value for NAD+ is 2fold higher than the KM-value of the wild-type enzyme | Homo sapiens |
ALDH1-5AA | aldehyde dehydrogenase class 1 with the addition of five amino acids at the C-terminus, KM-value for propionaldehyde is 67% of the KM-value of the wild-type enzyme, KM-value for NAD+ is 73% of the KM-value of the wild-type enzyme | Homo sapiens |
D80G | KM-value for propionaldehyde is 24% of the KM-value of the wild-type enzyme, KM-value for NAD+ is 32% of the KM-value of the wild-type enzyme | Homo sapiens |
D80G/S82A | the maximal velocity of the mutant enzyme is 7.5% of the activity of the wild-type enzyme | Homo sapiens |
R84Q | KM-value for propionaldehyde is 5.8fold higher than the Km-value of the wild-type enzyme, KM-value for NAD+ is 36% of the KM-value of the wild-type enzyme | Homo sapiens |
S82A | KM-value for propionaldehyde is 36% of the KM-value of the wild-type enzyme, KM-value for NAD+ is 15% of the KM-value of the wild-type enzyme | Homo sapiens |
General Stability | Organism |
---|---|
half-life of wild-type enzyme and mutant enzyme R84Q in 7 M urea is 10 min, the half-life of the double-mutant enzyme S82A is less than 2 min. At 2 M urea all of the proteins are present as a mixture of native and altered state | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
NAD+ | pH 7.5, 25°C, mutant enzyme S82A | Homo sapiens | |
0.0028 | - |
propionaldehyde | pH 7.5, 25°C, mutant enzyme D80G | Homo sapiens | |
0.0035 | - |
NAD+ | pH 7.5, 25°C, mutant enzyme D80G | Homo sapiens | |
0.004 | - |
NAD+ | pH 7.5, 25°C, mutant enzyme R84Q | Homo sapiens | |
0.0044 | - |
propionaldehyde | pH 7.5, 25°C, mutant enzyme S82A | Homo sapiens | |
0.008 | - |
NAD+ | pH 7.5, 25°C, mutant enzyme ALDH1-5AA | Homo sapiens | |
0.008 | - |
propionaldehyde | pH 7.5, 25°C, mutant enzyme ALDH1-5AA | Homo sapiens | |
0.011 | - |
NAD+ | pH 7.5, 25°C, wild-type enzyme | Homo sapiens | |
0.012 | - |
propionaldehyde | pH 7.5, 25°C, wild-type enzyme | Homo sapiens | |
0.022 | - |
NAD+ | pH 7.5, 25°C, mutant enzyme ALDH1-H3tail | Homo sapiens | |
0.031 | - |
propionaldehyde | pH 7.5, 25°C, mutant enzyme ALDH1-H3tail | Homo sapiens | |
0.07 | - |
propionaldehyde | pH 7.5, 25°C, mutant enzyme R84Q | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
propionaldehyde + NAD+ + H2O | - |
Homo sapiens | propionate + NADH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aldehyde dehydrogenase class 1 | - |
Homo sapiens |
ALDH1 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens |