Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 27% increased activity at 1 mM | Escherichia coli | |
dithiothreitol | 75% increased activity at 1 mM | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | complete inhibition at 1 mM | Escherichia coli | |
Co2+ | 50.3% residual activity at 1 mM | Escherichia coli | |
Cu2+ | 2.9% residual activity at 1 mM | Escherichia coli | |
EDTA | 85.3% residual activity at 1 mM | Escherichia coli | |
Fe2+ | 57.5% residual activity at 1 mM | Escherichia coli | |
Hg2+ | complete inhibition at 1 mM | Escherichia coli | |
K+ | 95.3% residual activity at 1 mM | Escherichia coli | |
Li+ | 2.8% residual activity at 1 mM | Escherichia coli | |
Mg2+ | 80.6% residual activity at 1 mM | Escherichia coli | |
Mn2+ | 84.8% residual activity at 1 mM | Escherichia coli | |
Na+ | 83.6% residual activity at 1 mM | Escherichia coli | |
NH3 | 79.8% residual activity at 1 mM | Escherichia coli | |
Ni2+ | 72.4% residual activity at 1 mM | Escherichia coli | |
Semicarbazide hydrochloride | 86.7% residual activity at 1 mM | Escherichia coli | |
Sodium bisulfite | 1.8% residual activity at 1 mM | Escherichia coli | |
Zn2+ | 7% residual activity at 1 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
3-hydroxypropionic acid | with NADH as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C | Escherichia coli | |
0.24 | - |
Valeraldehyde | with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C | Escherichia coli | |
0.29 | - |
3-hydroxypropionaldehyde | with NADP+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C | Escherichia coli | |
0.49 | - |
3-hydroxypropionaldehyde | with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C | Escherichia coli | |
0.68 | - |
Isovaleraldehyde | with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C | Escherichia coli | |
0.97 | - |
Butyraldehyde | with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C | Escherichia coli | |
1 | - |
acetaldehyde | with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C | Escherichia coli | |
1.21 | - |
propionaldehyde | with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C | Escherichia coli | |
5.37 | - |
benzaldehyde | with NADP+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
recombinant enzyme, SDS-PAGE | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA-HP resin column chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.42 | - |
crude extract, using 3-hydroxypropionaldehyde as a substrate, with NAD+ as a cofactor, at pH 8.0 and 37°C | Escherichia coli |
38.1 | - |
after 26.8fold purification, using 3-hydroxypropionaldehyde as a substrate, with NAD+ as a cofactor, at pH 8.0 and 37°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-furaldehyde + NAD+ + H2O | - |
Escherichia coli | 2-furanoic acid + NADH + H+ | - |
? | |
3-hydroxypropanoate + NADH + 2 H+ | - |
Escherichia coli | 3-hydroxypropionaldehyde + NAD+ + H2O | - |
r | |
3-hydroxypropionaldehyde + NAD+ + H2O | - |
Escherichia coli | 3-hydroxypropanoate + NADH + 2 H+ | - |
r | |
acetaldehyde + NAD+ + H2O | - |
Escherichia coli | acetate + NADH + H+ | - |
? | |
benzaldehyde + NADP+ + H2O | - |
Escherichia coli | benzoate + NADPH + H+ | - |
? | |
butyraldehyde + NAD+ + H2O | - |
Escherichia coli | butanoic acid + NADH + H+ | - |
? | |
isovaleraldehyde + NAD+ + H2O | - |
Escherichia coli | isovalerate + NADH + H+ | - |
? | |
additional information | no activity towards 3-hydroxypropionic acid in the presence of NADPH and formaldehyde | Escherichia coli | ? | - |
? | |
propionaldehyde + NAD+ + H2O | - |
Escherichia coli | propanoic acid + NADH + H+ | - |
? | |
valeraldehyde + NAD+ + H2O | - |
Escherichia coli | valerate + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ALDH | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
at pH 7 | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | AldH appears to be stable between 20°C and 50 C but becomes very unstable at or above 50°C | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
3-hydroxypropionic acid | with NADH as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C | Escherichia coli | |
4.91 | - |
3-hydroxypropionaldehyde | with NADP+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C | Escherichia coli | |
11.03 | - |
acetaldehyde | with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C | Escherichia coli | |
17.37 | - |
benzaldehyde | with NADP+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C | Escherichia coli | |
24.35 | - |
propionaldehyde | with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C | Escherichia coli | |
26.24 | - |
Valeraldehyde | with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C | Escherichia coli | |
26.95 | - |
Butyraldehyde | with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C | Escherichia coli | |
27.31 | - |
Isovaleraldehyde | with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C | Escherichia coli | |
28.54 | - |
3-hydroxypropionaldehyde | with NAD+ as cofactor, in 50 mM potassium phosphate buffer (pH 8.0), at 37°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | - |
Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | increasing or decreasing one pH unit from the optimal (pH 8.0) attenuates more than 70% of activity, pH 6.0 only 6% of the activity remains, pH values below 5.0 completely abolish activity | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | prefers NAD+ over NADP+ | Escherichia coli | |
NADP+ | prefers NAD+ over NADP+, in case of 3-hydroxypropionaldehyde, the activity is decreased by 73% when NAD+ is replaced with NADP+ | Escherichia coli |