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Literature summary for 1.2.1.3 extracted from

  • Zheng, T.; Olson, D.G.; Tian, L.; Bomble, Y.J.; Himmel, M.E.; Lo, J.; Hon, S.; Shaw, A.J.; van Dijken, J.P.; Lynd, L.R.
    Cofactor specificity of the bifunctional alcohol and aldehyde dehydrogenase (AdhE) in wild-type and mutant Clostridium thermocellum and Thermoanaerobacterium saccharolyticum (2015), J. Bacteriol., 197, 2610-2619.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strains LL346 and LL1040 Thermoanaerobacterium saccharolyticum
expressed in Escherichia coli strains LL346 and LL1040 Acetivibrio thermocellus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.088
-
NADH at pH 7.0 and 55°C Acetivibrio thermocellus
1.6
-
acetaldehyde at pH 7.0 and 55°C Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus
-
-
-
Acetivibrio thermocellus DSM 1313
-
-
-
Thermoanaerobacterium saccharolyticum
-
-
-
Thermoanaerobacterium saccharolyticum JW/SL-YS485
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Thermoanaerobacterium saccharolyticum
Ni-NTA column chromatography Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetaldehyde + NAD+ + H2O
-
Thermoanaerobacterium saccharolyticum acetate + NADH + H+
-
?
acetaldehyde + NAD+ + H2O
-
Acetivibrio thermocellus acetate + NADH + H+
-
?
acetaldehyde + NAD+ + H2O
-
Acetivibrio thermocellus DSM 1313 acetate + NADH + H+
-
?
acetaldehyde + NAD+ + H2O
-
Thermoanaerobacterium saccharolyticum JW/SL-YS485 acetate + NADH + H+
-
?
acetate + NADH + H+
-
Thermoanaerobacterium saccharolyticum acetaldehyde + NAD+ + H2O
-
?
acetate + NADH + H+
-
Acetivibrio thermocellus acetaldehyde + NAD+ + H2O
-
?
acetate + NADH + H+
-
Acetivibrio thermocellus DSM 1313 acetaldehyde + NAD+ + H2O
-
?
acetate + NADH + H+
-
Thermoanaerobacterium saccharolyticum JW/SL-YS485 acetaldehyde + NAD+ + H2O
-
?

Synonyms

Synonyms Comment Organism
ADH
-
Thermoanaerobacterium saccharolyticum
ADH
-
Acetivibrio thermocellus
AdhE bifunctional protein with alcohol dehydrogenase and aldehyde dehydrogenase activities Thermoanaerobacterium saccharolyticum
AdhE bifunctional protein with alcohol dehydrogenase and aldehyde dehydrogenase activities Acetivibrio thermocellus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
240
-
NADH at pH 7.0 and 55°C Acetivibrio thermocellus
240
-
acetaldehyde at pH 7.0 and 55°C Acetivibrio thermocellus

Cofactor

Cofactor Comment Organism Structure
NAD+ preferred cofactor Thermoanaerobacterium saccharolyticum
NAD+ preferred cofactor Acetivibrio thermocellus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
150
-
acetaldehyde at pH 7.0 and 55°C Acetivibrio thermocellus
2700
-
NADH at pH 7.0 and 55°C Acetivibrio thermocellus