Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strains LL346 and LL1040 | Thermoanaerobacterium saccharolyticum |
expressed in Escherichia coli strains LL346 and LL1040 | Acetivibrio thermocellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.088 | - |
NADH | at pH 7.0 and 55°C | Acetivibrio thermocellus | |
1.6 | - |
acetaldehyde | at pH 7.0 and 55°C | Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | - |
- |
- |
Acetivibrio thermocellus DSM 1313 | - |
- |
- |
Thermoanaerobacterium saccharolyticum | - |
- |
- |
Thermoanaerobacterium saccharolyticum JW/SL-YS485 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Thermoanaerobacterium saccharolyticum |
Ni-NTA column chromatography | Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetaldehyde + NAD+ + H2O | - |
Thermoanaerobacterium saccharolyticum | acetate + NADH + H+ | - |
? | |
acetaldehyde + NAD+ + H2O | - |
Acetivibrio thermocellus | acetate + NADH + H+ | - |
? | |
acetaldehyde + NAD+ + H2O | - |
Acetivibrio thermocellus DSM 1313 | acetate + NADH + H+ | - |
? | |
acetaldehyde + NAD+ + H2O | - |
Thermoanaerobacterium saccharolyticum JW/SL-YS485 | acetate + NADH + H+ | - |
? | |
acetate + NADH + H+ | - |
Thermoanaerobacterium saccharolyticum | acetaldehyde + NAD+ + H2O | - |
? | |
acetate + NADH + H+ | - |
Acetivibrio thermocellus | acetaldehyde + NAD+ + H2O | - |
? | |
acetate + NADH + H+ | - |
Acetivibrio thermocellus DSM 1313 | acetaldehyde + NAD+ + H2O | - |
? | |
acetate + NADH + H+ | - |
Thermoanaerobacterium saccharolyticum JW/SL-YS485 | acetaldehyde + NAD+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ADH | - |
Thermoanaerobacterium saccharolyticum |
ADH | - |
Acetivibrio thermocellus |
AdhE | bifunctional protein with alcohol dehydrogenase and aldehyde dehydrogenase activities | Thermoanaerobacterium saccharolyticum |
AdhE | bifunctional protein with alcohol dehydrogenase and aldehyde dehydrogenase activities | Acetivibrio thermocellus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
240 | - |
NADH | at pH 7.0 and 55°C | Acetivibrio thermocellus | |
240 | - |
acetaldehyde | at pH 7.0 and 55°C | Acetivibrio thermocellus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | preferred cofactor | Thermoanaerobacterium saccharolyticum | |
NAD+ | preferred cofactor | Acetivibrio thermocellus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
150 | - |
acetaldehyde | at pH 7.0 and 55°C | Acetivibrio thermocellus | |
2700 | - |
NADH | at pH 7.0 and 55°C | Acetivibrio thermocellus |