Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | about 140% activity at 5 mM | Sulfurisphaera tokodaii | |
dithiothreitol | about 150% activity at 5 mM | Sulfurisphaera tokodaii |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells | Sulfurisphaera tokodaii |
Protein Variants | Comment | Organism |
---|---|---|
C274A | the mutation leads to a drastic loss of the activity | Sulfurisphaera tokodaii |
C274S | the mutation leads to a drastic loss of the activity | Sulfurisphaera tokodaii |
E240A | the mutation leads to a drastic loss of the activity | Sulfurisphaera tokodaii |
E240S | the mutation leads to a drastic loss of the activity | Sulfurisphaera tokodaii |
E370Q | the mutation results in decreased activity (higher Km and lower kcat values toward NAD+ and acetaldehyde than the wild type enzyme) | Sulfurisphaera tokodaii |
I168A | the mutation results in no obvious change of kinetics properties toward acetaldehyde and a comparable increase of affinity toward NAD+ | Sulfurisphaera tokodaii |
K165Q | the mutation results in decreased activity (higher Km and lower kcat values toward NAD+ and acetaldehyde than the wild type enzyme) | Sulfurisphaera tokodaii |
N142A | the mutation results in decreased activity (higher Km and lower kcat values toward NAD+ and acetaldehyde than the wild type enzyme) | Sulfurisphaera tokodaii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
H2O2 | - |
Sulfurisphaera tokodaii | |
nitroglycerin | more than half of the original activity retained in the presence of 5.5 mM and approximate 80% of the activity is lost by the addition of 11 mM nitroglycerin | Sulfurisphaera tokodaii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.82 | - |
NAD+ | with Dl-glyceraldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
0.84 | - |
NAD+ | with acetaldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
3.81 | - |
NADP+ | with acetaldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
6.12 | - |
DL-glyceraldehyde 3-phosphate | in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
6.38 | - |
acetaldehyde | in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
8.47 | - |
NADP+ | with Dl-glyceraldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
8.51 | - |
DL-glyceraldehyde | in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 130% activity at 20 mM | Sulfurisphaera tokodaii | |
Li+ | 119% activity at 20 mM | Sulfurisphaera tokodaii | |
additional information | the enzyme is not influenced by 20 mM Mg2, Cu2+, Ca2+, Ba2+, Ni2+, Co2+, and Sr2+ | Sulfurisphaera tokodaii | |
Na+ | 122% activity at 20 mM | Sulfurisphaera tokodaii | |
NH4+ | 131% activity at 20 mM | Sulfurisphaera tokodaii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
4 * 50000, SDS-PAGE | Sulfurisphaera tokodaii |
51300 | - |
4 * 51300, calculated from amino acid sequence | Sulfurisphaera tokodaii |
200000 | - |
gel filtration | Sulfurisphaera tokodaii |
Organic Solvent | Comment | Organism |
---|---|---|
dithiothreitol | the enzyme is stable in dithiothreitol (90% of the original activity remaining) | Sulfurisphaera tokodaii |
Ethanol | the enzyme is stable in ethanol (90% of the original activity remaining) | Sulfurisphaera tokodaii |
isopropanol | the enzyme is stable in isopropanol (90% of the original activity remaining) | Sulfurisphaera tokodaii |
Methanol | the enzyme is stable in methanol (90% of the original activity remaining) | Sulfurisphaera tokodaii |
phenylmethylsulfonyl fluoride | the enzyme is stable in phenylmethylsulfonyl fluoride (90% of the original activity remaining) | Sulfurisphaera tokodaii |
SDS | the enzyme is stable in SDS (90% of the original activity remaining) | Sulfurisphaera tokodaii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurisphaera tokodaii | - |
- |
- |
Sulfurisphaera tokodaii 7 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity column chromatography | Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,3-dihydroxyacetone + NAD+ + H2O | 53.93% activity compared to acetaldehyde | Sulfurisphaera tokodaii | ? + NADH + H+ | - |
? | |
3-nitrobenzaldehyde + NAD+ + H2O | 32.98% activity compared to acetaldehyde | Sulfurisphaera tokodaii | 3-nitrobenzoate + NADH + H+ | - |
? | |
acetaldehyde + NAD+ + H2O | 100% activity with acetaldehyde and NAD+ | Sulfurisphaera tokodaii | acetate + NADH + H+ | - |
? | |
acetaldehyde + NAD+ + H2O | 100% activity with acetaldehyde and NAD+ | Sulfurisphaera tokodaii 7 | acetate + NADH + H+ | - |
? | |
acetaldehyde + NADP+ + H2O | low activity with NADP+ | Sulfurisphaera tokodaii | acetate + NADPH + H+ | - |
? | |
benzaldehyde + NAD+ + H2O | 35.5% activity compared to acetaldehyde | Sulfurisphaera tokodaii | benzoate + NADH + H+ | - |
? | |
benzaldehyde + NAD+ + H2O | 35.5% activity compared to acetaldehyde | Sulfurisphaera tokodaii 7 | benzoate + NADH + H+ | - |
? | |
butyraldehyde + NAD+ + H2O | 48.15% activity compared to acetaldehyde | Sulfurisphaera tokodaii | butyrate + NADH + H+ | - |
? | |
butyraldehyde + NAD+ + H2O | 48.15% activity compared to acetaldehyde | Sulfurisphaera tokodaii 7 | butyrate + NADH + H+ | - |
? | |
chloral + NAD+ + H2O | 2.67% activity compared to acetaldehyde | Sulfurisphaera tokodaii | trichloroacetate + NADH + H+ | - |
? | |
D-glyceraldehyde + NAD+ + H2O | 50.25% activity compared to acetaldehyde | Sulfurisphaera tokodaii | D-glycerate + NADH + H+ | - |
? | |
DL-glyceraldehyde + NAD+ + H2O | 77.27% activity compared to acetaldehyde | Sulfurisphaera tokodaii | DL-glycerate + NADH + H+ | - |
? | |
DL-glyceraldehyde + NAD+ + H2O | low activity with NADP+ | Sulfurisphaera tokodaii | DL-glycerate + NADH + H+ | - |
? | |
DL-glyceraldehyde 3-phosphate + NAD+ + H2O | 52.7% activity compared to acetaldehyde | Sulfurisphaera tokodaii | DL-glycerate 3-phosphate + NADH + H+ | - |
? | |
formaldehyde + NAD+ + H2O | 7.77% activity compared to acetaldehyde | Sulfurisphaera tokodaii | formate + NADH + H+ | - |
? | |
glycolaldehyde + NAD+ + H2O | 75.43% activity compared to acetaldehyde | Sulfurisphaera tokodaii | glycolate + NADH + H+ | - |
? | |
additional information | no activity with methanol, ethanol and isopropanol | Sulfurisphaera tokodaii | ? | - |
? | |
additional information | no activity with methanol, ethanol and isopropanol | Sulfurisphaera tokodaii 7 | ? | - |
? | |
phenylacetaldehyde + NAD+ + H2O | 4.21% activity compared to acetaldehyde | Sulfurisphaera tokodaii | phenylacetate + NADH + H+ | - |
? | |
propionaldehyde + NAD+ + H2O | 56.22% activity compared to acetaldehyde | Sulfurisphaera tokodaii | propionate + NADH + H+ | - |
? | |
propionaldehyde + NAD+ + H2O | 56.22% activity compared to acetaldehyde | Sulfurisphaera tokodaii 7 | propionate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 50000, SDS-PAGE | Sulfurisphaera tokodaii |
homotetramer | 4 * 51300, calculated from amino acid sequence | Sulfurisphaera tokodaii |
Synonyms | Comment | Organism |
---|---|---|
ALDH | - |
Sulfurisphaera tokodaii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Sulfurisphaera tokodaii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 90 | about 50% activity at 70°C, 100% activity at 80°C, about 80% activity at 90°C | Sulfurisphaera tokodaii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 90 | the enzyme shows no obvious loss of activity after heat-treatment at 70°C for 12 h, and more than 60% of the original activity remains after treatment at 80°C for 12 h. The half-life at 90°C is 4 h | Sulfurisphaera tokodaii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
NAD+ | with DL-glyceraldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
0.83 | - |
DL-glyceraldehyde 3-phosphate | in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
0.92 | - |
DL-glyceraldehyde | in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
1.14 | - |
acetaldehyde | in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
1.63 | - |
NADP+ | with DL-glyceraldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
2.05 | - |
NAD+ | with acetaldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
3.71 | - |
NADP+ | with acetaldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Sulfurisphaera tokodaii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | about 60% activity at pH 6.0, about 70% activity at pH 7.0, 100% activity pH 8.0, about 85% activity at pH 9.0 | Sulfurisphaera tokodaii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the enzyme can utilize both NAD+ and NADP+ as cofactors and shows a preference toward NAD+ | Sulfurisphaera tokodaii | |
NADP+ | the enzyme can utilize both NAD+ and NADP+ as cofactors and shows a preference toward NAD+ | Sulfurisphaera tokodaii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
DL-glyceraldehyde | in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
0.14 | - |
DL-glyceraldehyde 3-phosphate | in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
0.18 | - |
acetaldehyde | in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
0.19 | - |
NADP+ | with DL-glyceraldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
0.73 | - |
NAD+ | with DL-glyceraldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
0.98 | - |
NADP+ | with acetaldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii | |
2.45 | - |
NAD+ | with acetaldehyde as cosubstrate, in 50 mM Tris/HCl, at pH 8.0 and 80°C | Sulfurisphaera tokodaii |