6.3.1.20: lipoate-protein ligase

This is an abbreviated version!
For detailed information about lipoate-protein ligase, go to the full flat file.

Reaction

Synonyms

CTD, EC 2.7.7.63, LIP3, LipB, LipL1, lipoate ligase, lipoate ligase like protein, lipoate protein ligase, lipoate protein ligase B, lipoate-bound lipoate ligase 1, lipoate-protein ligase, lipoate-protein ligase A, lipoic acid ligase, lipoic acid ligase A, lipoic acid protein ligase, lipoic acid protein ligase A1, lipoyl ligase, lipoyl-protein ligase A, LPLA, LplA-LplB complex, LplA1, LplA1Ct, Lpla2, LplB, Oryza s. lipoate-protein ligase A, OsLPLA, PfLipL1

ECTree

     6 Ligases

         6.3 Forming carbon-nitrogen bonds

             6.3.1 Acid—ammonia (or amine) ligases (amide synthases)

                6.3.1.20 lipoate-protein ligase

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Results	in table
1	Activating Compound
10845	AA Sequence
5	Application
1	CAS Registry Number
14	Cloned(Commentary)
8	Cofactor
5	Crystallization (Commentary)
15	Disease/ Diagnostics
2	Expression
16	General Information
15	Inhibitors
4	kcat/KM [mM/s]
19	KM Value [mM]
10	Localization
18	Metals/Ions
13	Molecular Weight [Da]
18	Natural Substrates/ Products (Substrates)
43	Organism
1	Oxidation Stability
5	Pathway
2	PDB ID
4	pH Optimum
1	pH Range
2	pI Value
29	Protein Variants
11	Purification (Commentary)
4	Reaction
1	Reaction Type
35	Reference
1	Renatured (Commentary)
8	Source Tissue
5	Specific Activity [micromol/min/mg]
2	Storage Stability
71	Substrates and Products (Substrate)
8	Subunits
63	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
13	Turnover Number [1/s]

KM Value

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KM Value on EC 6.3.1.20 - lipoate-protein ligase

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

1.6

6-thio-octanoate

Escherichia coli

-

pH 7.0, 30°C

663919

0.0011 - 0.0155

apoH-protein

3 entries

-

0.0019 - 0.295

ATP

4 entries

0.0016 - 0.0017

DL-lipoic acid

2 entries

0.2

LplA acceptor peptide 1

Escherichia coli

-

-

704183

-

13.32

LplA acceptor peptide 2

Escherichia coli

-

-

704183

-

0.004 - 0.2143

octanoic acid

3 entries

0.00055 - 0.0089

R-lipoic acid

3 entries

0.013

selenolipoic acid

Escherichia coli

-

pH 7.0, 30°C

663919

0.0011

apoH-protein

Escherichia coli

-

mutant S72A, pH 7.0, 37°C

665657

-

0.0012

apoH-protein

Escherichia coli

-

wild-type, pH 7.0, 37°C

665657

-

0.0155

apoH-protein

Escherichia coli

-

mutant R140A, pH 7.0, 37°C

665657

-

0.0019

ATP

Escherichia coli

-

pH 7.0, 30°C

663919

0.0137

ATP

Escherichia coli

-

mutant R140A, pH 7.0, 37°C

665657

0.0158

ATP

Escherichia coli

-

wild-type, pH 7.0, 37°C

665657

0.295

ATP

Escherichia coli

-

mutant S72A, pH 7.0, 37°C

665657

0.0016

DL-lipoic acid

Escherichia coli

-

pH 7.0, 30°C

663919

0.0017

DL-lipoic acid

Escherichia coli

-

pH 7.0, 30°C

663919

0.004

octanoic acid

Escherichia coli

-

mutant S221P

704311

0.0104

octanoic acid

Escherichia coli

-

mutant V19L

704311

0.2143

octanoic acid

Escherichia coli

-

wild type enzyme

704311

0.00055

R-lipoic acid

Escherichia coli

-

mutant R140A, pH 7.0, 37°C

665657

0.0045

R-lipoic acid

Escherichia coli

-

wild-type, pH 7.0, 37°C

665657

0.0089

R-lipoic acid

Escherichia coli

-

mutant S72A, pH 7.0, 37°C

665657