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Literature summary for 6.3.1.20 extracted from

  • Green, D.E.; Morris, T.W.; Green, J.; Cronan, J.E., Jr.; Guest, J.R.
    Purification and properties of the lipoate protein ligase of Escherichia coli (1995), Biochem. J., 309, 853-862.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.1.20

Inhibitors

Inhibitors Comment Organism Structure
6-seleno-octanoate 0.28 mM, complete inhibition, reactivation by reducing agents Escherichia coli
8-thiooctanoate 0.28 mM, 50% inhibition, reactivation by reducing agents Escherichia coli
Cu2+ 0.13 mM, 50% inhibition Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
 DL-lipoic acid pH 7.0, 30°C Escherichia coli
0.0017
-
 DL-lipoic acid pH 7.0, 30°C Escherichia coli
0.0019
-
 ATP pH 7.0, 30°C Escherichia coli
0.013
-
 selenolipoic acid pH 7.0, 30°C Escherichia coli
1.6
-
 6-thio-octanoate pH 7.0, 30°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ may substitute for Mg2+ Escherichia coli
Fe2+ may substitute for Mg2+ Escherichia coli
Mg2+ Km-value 0.15 mM Escherichia coli
Mn2+ may substitute for Mg2+ Escherichia coli
Ni2+ may substitute for Mg2+ Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 1 * 38000, SDS-PAGE Escherichia coli
41700
-
 gel filtration Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Oxidation Stability

Oxidation Stability Organism
slow oxidation to an inactive form containing at least one intramolecular disulfide bond, reactivation by reducing agent Escherichia coli

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Renatured (Commentary)

Renatured (Comment) Organism
reactivation of inactive form containing at least one intramolecular disulfide bond, or of enzyme inactivated by substrate-analogues, by reducing agent Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
27.6
-
 pH 7.0, 30°C Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, stable for several months Escherichia coli
4°C, slow oxidation to an inactive form containing at least one intramolecular disulfide bond, reactivation by reducing agent Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 6-thio-octanoic acid at 326% of the rate with DL-lipoic acid Escherichia coli diphosphate + 6-thio-octanoyl-AMP
-
 ?
ATP + 8-methyl-lipoic acid at 73% of the rate with DL-lipoic acid Escherichia coli diphosphate + 8-methyl-lipoyl-AMP
-
 ?
ATP + D-lipoic acid at 83% of the rate with DL-lipoic acid Escherichia coli diphosphate + D-lipoyl-AMP
-
 ?
ATP + dihydro-DL-lipoic acid at 80% of the rate with DL-lipoic acid Escherichia coli diphosphate + dihydro-DL-lipoyl-AMP
-
 ?
ATP + DL-lipoic acid
-
 Escherichia coli diphosphate + DL-lipoyl-AMP
-
 ?
ATP + L-lipoic acid at 36% of the rate with DL-lipoic acid Escherichia coli diphosphate + L-lipoyl-AMP
-
 ?
ATP + octanoic acid at 13% of the rate with DL-lipoic acid Escherichia coli diphosphate + octanoyl-AMP
-
 ?
ATP + selenolipoic acid at 12% of the rate with DL-lipoic acid Escherichia coli diphosphate + selenolipoyl-AMP
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 38000, SDS-PAGE Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 more than 70% of maximum activity within this range Escherichia coli

pI Value

Organism Comment pI Value Maximum pI Value
Escherichia coli isoelectric focusing
-
 5.8
Escherichia coli calculated
-
 6