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Literature summary for 6.3.1.20 extracted from

  • Fujiwara, K.; Toma, S.; Okamura-Ikeda, K.; Motokawa, Y.; Nakagawa, A.; Taniguchi, H.
    Crystal structure of lipoate-protein ligase A from Escherichia coli. Determination of the lipoic acid-binding site (2005), J. Biol. Chem., 280, 33645-33651.
    View publication on PubMed
Search for more literature for 6.3.1.20

Crystallization (Commentary)

Crystallization (Comment) Organism
alone and in complex with lipoic acid Escherichia coli

Protein Variants

Protein Variants Comment Organism
R140A 12fold increase in Km-value for apoH-protein Escherichia coli
S72A great increase in Km-value for ATP Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00055
-
 R-lipoic acid mutant R140A, pH 7.0, 37°C Escherichia coli
0.0011
-
 apoH-protein mutant S72A, pH 7.0, 37°C Escherichia coli
0.0012
-
 apoH-protein wild-type, pH 7.0, 37°C Escherichia coli
0.0045
-
 R-lipoic acid wild-type, pH 7.0, 37°C Escherichia coli
0.0089
-
 R-lipoic acid mutant S72A, pH 7.0, 37°C Escherichia coli
0.0137
-
 ATP mutant R140A, pH 7.0, 37°C Escherichia coli
0.0155
-
 apoH-protein mutant R140A, pH 7.0, 37°C Escherichia coli
0.0158
-
 ATP wild-type, pH 7.0, 37°C Escherichia coli
0.295
-
 ATP mutant S72A, pH 7.0, 37°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-lipoic acid + Escherichia coli apoH-protein
-
 Escherichia coli diphosphate + AMP + Escherichia coli (R)-lipoyl-apoH-protein
-
 ?
ATP + lipoate + apoH-protein
-
 Escherichia coli ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.006
-
 R-lipoic acid mutant R140A, pH 7.0, 37°C Escherichia coli
0.0084
-
 ATP mutant R140A, pH 7.0, 37°C Escherichia coli
0.028
-
 apoH-protein mutant R140A, pH 7.0, 37°C Escherichia coli
0.133
-
 ATP wild-type, pH 7.0, 37°C Escherichia coli
0.16
-
 ATP mutant S72A, pH 7.0, 37°C Escherichia coli
0.178
-
 R-lipoic acid mutant S72A, pH 7.0, 37°C Escherichia coli
0.212
-
 apoH-protein mutant S72A, pH 7.0, 37°C Escherichia coli
0.219
-
 R-lipoic acid wild-type, pH 7.0, 37°C Escherichia coli
0.258
-
 apoH-protein wild-type, pH 7.0, 37°C Escherichia coli