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6.3.1.20: lipoate-protein ligase

This is an abbreviated version!
For detailed information about lipoate-protein ligase, go to the full flat file.

Word Map on EC 6.3.1.20

Reaction

ATP
+
(R)-lipoate
 +
a [lipoyl-carrier protein]-L-lysine
=
a [lipoyl-carrier protein]-N6-(lipoyl)lysine
 +
AMP
 +
diphosphate

Synonyms

CTD, EC 2.7.7.63, LIP3, LipB, LipL1, lipoate ligase, lipoate ligase like protein, lipoate protein ligase, lipoate protein ligase B, lipoate-bound lipoate ligase 1, lipoate-protein ligase, lipoate-protein ligase A, lipoic acid ligase, lipoic acid ligase A, lipoic acid protein ligase, lipoic acid protein ligase A1, lipoyl ligase, lipoyl-protein ligase A, LPLA, LplA-LplB complex, LplA1, LplA1Ct, Lpla2, LplB, Oryza s. lipoate-protein ligase A, OsLPLA, PfLipL1

ECTree

     6 Ligases
         6.3 Forming carbon-nitrogen bonds
             6.3.1 Acid—ammonia (or amine) ligases (amide synthases)
                6.3.1.20 lipoate-protein ligase

Cloned

Cloned on EC 6.3.1.20 - lipoate-protein ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in an Escherichia coli ligase-deficient strain QC146
-
expressed in Escherichia coli
expressed in Escherichia coli as a His-tagged fusion protein
expressed in Pichia pastoris strain GS115 and in Escherichia coli strain QC146
-
expressed in the lipoylation-deficient Escherichia coli mutant TM137 and in Nicotiana tabacum protoplasts
-
from cDNA library in pTrcHis2A for expression and functional complementation in Escherichia coli strain TM137 (lplA/lipB null mutant of strain JK1)
-
gene lplA, recombinant expression of N-terminally His- and GST-tagged enzyme LplAW37V in Escherichia coli strain BL21
in Escherichia coli
-
LplA1: in pAM401 for complementation in Listeria monocytogenes mutant strains DP-L4263 and 10403S, in ptac85 for IPTG-inducible expression in Escherichia coli wild-type strain JK1 or strain TM131 lacking endogenous LplA, LplA2: from bacterial genome (10403S) in pKSV7 for allelic exchange (homologous recombination), in ptac85 for IPTG-inducible expression in Escherichia coli wild-type strain JK1 or strain TM131 lacking endogenous LplA
-
recombinant expression of His6-tagged enzyme in HEK-293 cells
-
recombinant expression of truncated enzyme mutants from plasmid pMALcHT, encoding a maltose-binding protein (MBP) followed by a linker region composed of a tobacco etch virus (TEV) protease cut site and a six histidine affinity tag, in Escherichia coli strain BL21-Star (DE3), recombinnat expression of wild-type and truncated mutant enzymes in lipoylation-deficient Escherichia coli strain JEG3. Structure comparisons, overview
recombinant expression of wild-type enzyme and enzyme mutant LplAW37V
-