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Literature summary for 6.3.1.20 extracted from

  • Ewald, R.; Hoffmann, C.; Florian, A.; Neuhaus, E.; Fernie, A.R.; Bauwe, H.
    Lipoate-protein ligase and octanoyltransferase are essential for protein lipoylation in mitochondria of Arabidopsis (2014), Plant Physiol., 165, 978-990.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expressed in the lipoylation-deficient Escherichia coli mutant TM137 and in Nicotiana tabacum protoplasts Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Arabidopsis thaliana 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-lipoate Arabidopsis thaliana
-
 lipoyl-AMP + diphosphate
-
 ?
lipoyl-AMP + [GLDH1 protein]-L-lysine Arabidopsis thaliana
-
 [GLDH1 protein]-N6-(lipoyl)lysine + AMP
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Arabidopsis thaliana
-
root
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-lipoate
-
 Arabidopsis thaliana lipoyl-AMP + diphosphate
-
 ?
lipoyl-AMP + [GLDH1 protein]-L-lysine
-
 Arabidopsis thaliana [GLDH1 protein]-N6-(lipoyl)lysine + AMP
-
 ?
lipoyl-AMP + [pyruvate dehydrogenase-E2]-L-lysine
-
 Arabidopsis thaliana [pyruvate dehydrogenase-E2]-N6-(lipoyl)lysine + AMP
-
 ?
lipoyl-AMP + [TM137 soluble protein]-L-lysine
-
 Arabidopsis thaliana [TM137 soluble protein]-N6-(lipoyl)lysine + AMP
-
 ?
additional information the enzyme uses octanoyl-nucleoside monophosphate and possibly other donor substrates for the octanoylation of mitochondrial pyruvate dehydrogenase-E2 (essentially) and glycine decarboxylase H-protein, and shows no reactivity with bacterial and possibly plant alpha- ketoglutarate dehydrogenase-E2 Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
LPLA
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function the enzyme is essential for embryo development and necessary for the lipoylation of pyruvate dehydrogenase-E2 proteins in leaves and roots Arabidopsis thaliana