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Literature summary for 6.3.1.20 extracted from

  • Kang, S.G.; Jeong, H.K.; Lee, E.; Natarajan, S.
    Characterization of a lipoate-protein ligase A gene of rice (Oryza sativa L.) (2007), Gene, 393, 53-61.
    View publication on PubMed
Search for more literature for 6.3.1.20

Cloned(Commentary)

Cloned (Comment) Organism
from cDNA library in pTrcHis2A for expression and functional complementation in Escherichia coli strain TM137 (lplA/lipB null mutant of strain JK1) Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lipoic acid + ATP + apoprotein Oryza sativa two-step reaction of protein lipoylation at lysine residues with lipoyl-AMP intermediate diphosphate + AMP + N6-(lipoyl)-lysine
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
 Japonica type
-

Source Tissue

Source Tissue Comment Organism Textmining
flower developing, moderate expression, Northern blot Oryza sativa
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leaf northern blot Oryza sativa
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additional information transcript levels not influenced by reduced temperature (4°C), high salt (100 mM NaCl) or 72 h treatment with 100 micromolar kinetin, 100 micromolar gibberellic acid or 50 micromolar alpha-naphthalene acetic acid Oryza sativa
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root less abundant, Northern blot Oryza sativa
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seed developing seed, increasing level at 3 to 5 days after pollination followed by decreasing for 15 days, Northern blot Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lipoic acid + ATP + apoprotein two-step reaction of protein lipoylation at lysine residues with lipoyl-AMP intermediate Oryza sativa diphosphate + AMP + N6-(lipoyl)-lysine
-
 ?
lipoic acid + ATP + apoprotein complementation assay in LplA-deficient Escherichia coli strain TM137 (2 days, 37°C) using exogenous lipoate Oryza sativa diphosphate + AMP + N6-(lipoyl)-lysine detection of lipoylated proteins in Western blot ?

Synonyms

Synonyms Comment Organism
lipoate-protein ligase A
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 Oryza sativa
lipoyl-protein ligase A
-
 Oryza sativa
Oryza s. lipoate-protein ligase A
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 Oryza sativa
OsLPLA on chromosome 8 of Oryza sativa Oryza sativa