6.1.1.15: proline-tRNA ligase

This is an abbreviated version!
For detailed information about proline-tRNA ligase, go to the full flat file.

Reaction

Synonyms

bifunctional aminoacyl-tRNA synthetase, bifunctional glutamate/proline-tRNA ligase, class II prolyl-tRNA synthetase, class II ProRS, dual-specificity prolyl-cysteinyl-tRNA synthetase, dual-specificity prolyl-tRNA synthetase, EPRS, Global RNA synthesis factor, GluProRS, glutamyl-/prolyl-tRNA synthetase, glutamyl-prolyl tRNA synthetase, glutamyl-prolyl-tRNA synthetase, More, MSMEG_2621, Pro-tRNA synthetase, ProCysRS, Proline translase, Proline--tRNA ligase, proline/cysteine-tRNA ligase, Prolyl RNA synthetase, prolyl tRNA synthetase, prolyl-cysteinyl-tRNA synthetase, Prolyl-transfer ribonucleate synthetase, Prolyl-transfer ribonucleic acid synthetase, Prolyl-transfer RNA synthetase, Prolyl-tRNA synthetase, ProRS, ProRS-Cyt, ProRS-Org, ProRSTT, proS, PRS

ECTree

     6 Ligases

         6.1 Forming carbon-oxygen bonds

             6.1.1 Ligases forming aminoacyl-tRNA and related compounds

                6.1.1.15 proline-tRNA ligase

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Results	in table
2	Activating Compound
53727	AA Sequence
3	Application
1	CAS Registry Number
33	Cloned(Commentary)
67	Cofactor
14	Crystallization (Commentary)
63	Disease/ Diagnostics
7	Expression
19	General Information
28	General Stability
35	IC50 Value
171	Inhibitors
27	kcat/KM [mM/s]
4	Ki Value [mM]
131	KM Value [mM]
17	Localization
29	Metals/Ions
11	Molecular Weight [Da]
88	Natural Substrates/ Products (Substrates)
232	Organism
4	Pathway
144	PDB ID
43	pH Optimum
2	pH Range
1	pH Stability
6	Posttranslational Modification
83	Protein Variants
47	Purification (Commentary)
13	Reaction
66	Reaction Type
133	Reference
30	Source Tissue
15	Specific Activity [micromol/min/mg]
3	Storage Stability
294	Substrates and Products (Substrate)
21	Subunits
450	Synonyms
1	Systematic Name
36	Temperature Optimum [°C]
1	Temperature Range [°C]
37	Temperature Stability [°C]
83	Turnover Number [1/s]

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

3.7 - 81

ATP

4 entries

0.056 - 21

cis-4-hydroxyproline

2 entries

0.11 - 3.52

L-alanine

6 entries

0.003 - 0.8

L-cysteine

15 entries

0.024 - 210

L-proline

42 entries

15

trans-4-hydroxyproline

Escherichia coli

-

pH 7.0, 37°C, recombinant wild-type enzyme, amino acid activation

674646

0.0091 - 10.8

tRNAPro

12 entries

additional information

additional information

Escherichia coli

-

the rate of AMP formation of K279A ProRS in the presence of alanine is 0.034 s-1, which is at least 20 times faster than the rate of nonenzymatic hydrolysis

693220

-

3.7

ATP

Escherichia coli

-

mutant C443S, ATP-diphosphate exchange

164

15

ATP

Escherichia coli

-

mutant C443G, ATP-diphosphate exchange

164

28

ATP

Escherichia coli

-

mutant C443A, ATP-diphosphate exchange

164

81

ATP

Escherichia coli

-

wild-type enzyme, ATP-diphosphate exchange

164

0.056

cis-4-hydroxyproline

Escherichia coli

-

pH 7.0, 37°C, recombinant mutant EcDELTAINS Gly12Ser4, amino acid activation

674646

21

cis-4-hydroxyproline

Escherichia coli

-

pH 7.0, 37°C, recombinant wild-type enzyme, amino acid activation

674646

0.11

L-alanine

Homo sapiens

-

37°C

652154

1.7

L-alanine

Escherichia coli

-

37°C

652154

1.7

L-alanine

Escherichia coli

-

pH 7.0, 37°C, recombinant wild-type enzyme, amino acid activation

674646

2.18

L-alanine

Escherichia coli

P16659

mutant enzyme G217A, at pH 7.0 and 22°C

726978

3.26

L-alanine

Escherichia coli

P16659

mutant enzyme E218A, at pH 7.0 and 22°C

726978

3.52

L-alanine

Escherichia coli

P16659

wild type enzyme, at pH 7.0 and 22°C

726978

0.003

L-cysteine

Saccharomyces cerevisiae

-

recombinant enzyme, pH 7.2, 37°C

652298

0.014

L-cysteine

Deinococcus radiodurans

-

recombinant enzyme, pH 7.2, 37°C

652298

0.02

L-cysteine

Methanocaldococcus jannaschii

Q58635

aminoacylation reaction, recombinant wild-type enzyme, pH 7.0, 70°C

650058

0.02

L-cysteine

Methanothermobacter thermautotrophicus

-

recombinant enzyme, pH 7.2, 60°C

652298

0.022

L-cysteine

Escherichia coli

-

recombinant enzyme, pH 7.2, 37°C

652298

0.025

L-cysteine

Rhodopseudomonas palustris

-

recombinant enzyme, pH 7.2, 37°C

652298

0.04

L-cysteine

Thermus thermophilus

-

recombinant enzyme, pH 7.2, 60°C

652298

0.065

L-cysteine

Novosphingobium aromaticivorans

-

recombinant enzyme, pH 7.2, 37°C

652298

0.083

L-cysteine

Cytophaga hutchinsonii

-

recombinant enzyme, pH 7.2, 37°C

652298

0.09

L-cysteine

Aquifex aeolicus

-

recombinant enzyme, pH 7.2, 60°C

652298

0.11

L-cysteine

Magnetospirillum magnetotacticum

-

recombinant enzyme, pH 7.2, 37°C

652298

0.12

L-cysteine

Borreliella burgdorferi

-

recombinant enzyme, pH 7.2, 37°C

652298

0.19

L-cysteine

Methanocaldococcus jannaschii

-

recombinant enzyme, pH 7.2, 60°C

652298

0.39

L-cysteine

Acetoanaerobium sticklandii

-

recombinant enzyme, pH 7.2, 37°C

652298

0.8

L-cysteine

Methanococcus maripaludis

Q6LZD3

pH 7.0, 37°C

653662

0.024

L-proline

Escherichia coli

-

pH 7.5, 37°C, mutant K279A

693220

0.09

L-proline

Methanocaldococcus jannaschii

Q58635

aminoacylation reaction, recombinant wild-type enzyme, pH 7.0, 70°C

650058

0.131

L-proline

Escherichia coli

-

mutant enzyme F415A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C

727022

0.23

L-proline

Escherichia coli

-

pH 7.5, 37°C, mutant K279A

693220

0.61

L-proline

Escherichia coli

-

mutant enzyme N305A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C

727022

0.9

L-proline

Methanococcus maripaludis

Q6LZD3

pH 7.0, 37°C

653662

3.37

L-proline

Escherichia coli

P16659

mutant enzyme G217A, at pH 7.0 and 22°C

726978

3.4

L-proline

Methanothermobacter thermautotrophicus

-

recombinant enzyme, pH 7.2, 60°C

652298

4.4

L-proline

Escherichia coli

P16659

mutant enzyme E218A, at pH 7.0 and 22°C

726978

4.4

L-proline

Escherichia coli

-

mutant enzyme E218A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C

727022

4.6

L-proline

Escherichia coli

-

mutant enzyme E303D, at pH 7.5 and 22°C

727041

5.1

L-proline

Escherichia coli

-

mutant enzyme E303K, at pH 7.5 and 22°C

727041

5.2

L-proline

Escherichia coli

-

mutant enzyme E303A, at pH 7.5 and 22°C

727041

5.6

L-proline

Saccharomyces cerevisiae

-

recombinant enzyme, pH 7.2, 37°C

652298

5.7

L-proline

Escherichia coli

-

mutant enzyme K297E, at pH 7.5 and 22°C

727041

5.9

L-proline

Escherichia coli

-

mutant enzyme K297E/E303K, at pH 7.5 and 22°C

727041

6.7

L-proline

Escherichia coli

-

mutant enzyme E234A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C

727022

6.98

L-proline

Escherichia coli

-

mutant enzyme D198A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C

727022

7.3

L-proline

Escherichia coli

-

mutant enzyme H302A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C

727022

10.7

L-proline

Escherichia coli

-

mutant enzyme H302A/G412A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C

727022

12

L-proline

Escherichia coli

-

pH 7.0, 37°C, recombinant mutant EcDELTAINS Gly12Ser4, amino acid activation

674646

12.6

L-proline

Escherichia coli

-

wild type enzyme, at pH 7.5 and 22°C

727041

12.6

L-proline

Escherichia coli

-

wild type enzyme, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C

727022

12.7

L-proline

Escherichia coli

P16659

wild type enzyme, at pH 7.0 and 22°C

726978

12.8

L-proline

Escherichia coli

-

mutant enzyme G412A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C

727022

13.2

L-proline

Arabidopsis thaliana

-

isozyme ProRS-Org, at pH 7.2 and 30°C

746082

14

L-proline

Escherichia coli

-

recombinant enzyme, pH 7.2, 37°C

652298

14

L-proline

Rhodopseudomonas palustris

-

recombinant enzyme, pH 7.2, 37°C

652298

15

L-proline

Aquifex aeolicus

-

recombinant enzyme, pH 7.2, 60°C

652298

19

L-proline

Cytophaga hutchinsonii

-

recombinant enzyme, pH 7.2, 37°C

652298

26

L-proline

Novosphingobium aromaticivorans

-

recombinant enzyme, pH 7.2, 37°C

652298

32

L-proline

Deinococcus radiodurans

-

recombinant enzyme, pH 7.2, 37°C

652298

34

L-proline

Borreliella burgdorferi

-

recombinant enzyme, pH 7.2, 37°C

652298

35

L-proline

Magnetospirillum magnetotacticum

-

recombinant enzyme, pH 7.2, 37°C

652298

35

L-proline

Thermus thermophilus

-

recombinant enzyme, pH 7.2, 60°C

652298

57.5

L-proline

Arabidopsis thaliana

-

isozyme ProRS-Cyt, at pH 7.2 and 30°C

746082

60

L-proline

Acetoanaerobium sticklandii

-

recombinant enzyme, pH 7.2, 37°C

652298

63

L-proline

Methanocaldococcus jannaschii

-

recombinant enzyme, pH 7.2, 60°C

652298

70

L-proline

Escherichia coli

-

37°C

652154

70

L-proline

Escherichia coli

-

pH 7.0, 37°C, recombinant wild-type enzyme, amino acid activation

674646

80

L-proline

Homo sapiens

-

37°C

652154

210

L-proline

Methanocaldococcus jannaschii

-

60°C, pH not specified in the publication, ATP-diphosphate exchange assay

652154

0.0091

tRNAPro

Escherichia coli

-

pH 7.0, 37°C, recombinant mutant EcDELTAINS Gly12Ser4, aminoacylation

674646

0.239

tRNAPro

Escherichia coli

-

pH 7.0, 37°C, recombinant wild-type enzyme, aminoacylation

674646

0.4

tRNAPro

Methanocaldococcus jannaschii

-

pH 7.0, 55°C, C-terminal deletion mutant

661767

0.58

tRNAPro

Methanocaldococcus jannaschii

Q58635

pH 7.0, 37°C

728125

1.6

tRNAPro

Methanocaldococcus jannaschii

-

pH 7.0, 55°C, wild-type

661767

1.8

tRNAPro

Methanothermobacter thermautotrophicus

-

50°C, pH 7.5

662383

1.8

tRNAPro

Deinococcus radiodurans

-

steady-state

675395

2.9

tRNAPro

Methanothermobacter thermautotrophicus

-

presence of bovine serum albumine, 50°C, pH 7.5

662383

5.1

tRNAPro

Methanothermobacter thermautotrophicus

-

presence of leucyl-tRNA synthetase, 50°C, pH 7.5

662383

6.9

tRNAPro

Methanothermobacter thermautotrophicus

-

presence of bovine serum albumine and leucyl-tRNA synthetase, 50°C, pH 7.5

662383

9.8

tRNAPro

Deinococcus radiodurans

-

single turnover overall aminoacylation

675395

10.8

tRNAPro

Deinococcus radiodurans

-

single turnover of the transfer step

675395