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6.1.1.15: proline-tRNA ligase

This is an abbreviated version!
For detailed information about proline-tRNA ligase, go to the full flat file.

Word Map on EC 6.1.1.15

Reaction

ATP
+
L-proline
 +
tRNAPro
=
AMP
 +
diphosphate
 +
L-prolyl-tRNAPro

Synonyms

bifunctional aminoacyl-tRNA synthetase, bifunctional glutamate/proline-tRNA ligase, class II prolyl-tRNA synthetase, class II ProRS, dual-specificity prolyl-cysteinyl-tRNA synthetase, dual-specificity prolyl-tRNA synthetase, EPRS, Global RNA synthesis factor, GluProRS, glutamyl-/prolyl-tRNA synthetase, glutamyl-prolyl tRNA synthetase, glutamyl-prolyl-tRNA synthetase, More, MSMEG_2621, Pro-tRNA synthetase, ProCysRS, Proline translase, Proline--tRNA ligase, proline/cysteine-tRNA ligase, Prolyl RNA synthetase, prolyl tRNA synthetase, prolyl-cysteinyl-tRNA synthetase, Prolyl-transfer ribonucleate synthetase, Prolyl-transfer ribonucleic acid synthetase, Prolyl-transfer RNA synthetase, Prolyl-tRNA synthetase, ProRS, ProRS-Cyt, ProRS-Org, ProRSTT, proS, PRS

ECTree

     6 Ligases
         6.1 Forming carbon-oxygen bonds
             6.1.1 Ligases forming aminoacyl-tRNA and related compounds
                6.1.1.15 proline-tRNA ligase

Cloned

Cloned on EC 6.1.1.15 - proline-tRNA ligase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21(DE3)Star cells
expressed in Escherichia coli BL21-(DE3)R3 Rosetta cells
expressed in Escherichia coli BL21-Codon Plus (DE3) cells
-
expressed in Escherichia coli BL21-CodonPlus (DE3) cells and HEK-293T cells
expressed in Escherichia coli Rosetta 2 (DE3) pLysS cells
expressed in Escherichia coli SG13009 (pREP4) cells
expression as His-tagged enzyme in Escherichia coli
-
expression in Escherichia coli
expression of C-terminally His-tagged, full-length EPRS
-
expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
expression of ProRS and leucyl-tRNA synthetase, LeuRS, in a Saccharomyces cerevisiae two-hybrid system, expression of His6-tagged ProRS in Escherichia coli strain BL21(DE3)
-
expression of the enzyme in Escherichia coli strain M15
expression of the His-tagged enzyme in Escherichia coli BL21(DE3)
gene EPRS, quantitative PCR-based expression analysis
-
gene proS, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in conditional expression strains of Mycobacterium smegmatis strain mc2155 from pAZI9452 vector
gene proS, expression in Escherichia coli strain BL21(DE3)
gene proS, expression in Escherichia coli strain SG13009
-
gene proS, overexpression in Escherichia coli
-
gene proS, overexpression of His-tagged wild-type ProRS and truncation mutant ScDELTA183
-
gene proS, overexpression of wild-type and mutant His-tagged ProRS
-
heterologous archaeal prolyl-tRNA/prolyl-tRNA synthase pair, Archaeoglobus fulgidus tRNAPro (AftRNAPro)/Pyrococcus horikoshii ProRS (PhProRS), for UAA mutagenesis in Escherichia coli
-
overexpressed in Escherichia coli
overexpression of wild-type and C-terminally truncated enzymes as His-tagged proteins in Escherichia coli
-
quantitative real-time PCR enzyme expression analysis, recombinant expression of tagged wild-type and mutant enzymes in Escherichia coli strain Escherichia coli BL21-CodonPlus (DE3)-RIPL
recombinant His-tagged wild-type and mutants from overexpressing Escherichia coli BL21