Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli SG13009 (pREP4) cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E303A | the mutation results in 3fold decrease in L-proline activation. The mutant exhibits a small decrease in the aminoacylation efficiency | Escherichia coli |
E303D | the mutation results in 3.1fold decrease in L-proline activation. The mutant exhibits a small decrease in the aminoacylation efficiency | Escherichia coli |
E303K | the mutation results in 4.2fold decrease in L-proline activation. The mutant exhibits a small decrease in the aminoacylation efficiency | Escherichia coli |
K279E | the mutation results in 2.7fold reduced L-proline activation. The mutant exhibits wild type aminoacylation efficiency | Escherichia coli |
K279E/E303K | the mutant shows 2.3fold reduced L-proline activation. The mutant exhibits wild type aminoacylation efficiency | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
L-proline | wild type enzyme, at pH 7.5 and 22°C | Escherichia coli | |
0.19 | - |
L-proline | mutant enzyme K297E/E303K, at pH 7.5 and 22°C | Escherichia coli | |
0.199 | - |
L-proline | mutant enzyme E303D, at pH 7.5 and 22°C | Escherichia coli | |
0.216 | - |
L-proline | mutant enzyme E303A, at pH 7.5 and 22°C | Escherichia coli | |
0.22 | - |
L-proline | mutant enzyme K297E, at pH 7.5 and 22°C | Escherichia coli | |
0.3 | - |
L-proline | mutant enzyme E303K, at pH 7.5 and 22°C | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-alanine + tRNAPro | Escherichia coli | - |
AMP + diphosphate + L-alanyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | Escherichia coli | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Talon cobalt affinity resin column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-alanine + tRNAPro | - |
Escherichia coli | AMP + diphosphate + L-alanyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | - |
Escherichia coli | AMP + diphosphate + L-prolyl-tRNAPro | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Prolyl-tRNA synthetase | - |
Escherichia coli |
ProRS | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.6 | - |
L-proline | mutant enzyme E303D, at pH 7.5 and 22°C | Escherichia coli | |
5.1 | - |
L-proline | mutant enzyme E303K, at pH 7.5 and 22°C | Escherichia coli | |
5.2 | - |
L-proline | mutant enzyme E303A, at pH 7.5 and 22°C | Escherichia coli | |
5.7 | - |
L-proline | mutant enzyme K297E, at pH 7.5 and 22°C | Escherichia coli | |
5.9 | - |
L-proline | mutant enzyme K297E/E303K, at pH 7.5 and 22°C | Escherichia coli | |
12.6 | - |
L-proline | wild type enzyme, at pH 7.5 and 22°C | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17 | - |
L-proline | mutant enzyme E303K, at pH 7.5 and 22°C | Escherichia coli | |
23 | - |
L-proline | mutant enzyme E303D, at pH 7.5 and 22°C | Escherichia coli | |
24 | - |
L-proline | mutant enzyme E303A, at pH 7.5 and 22°C | Escherichia coli | |
26 | - |
L-proline | mutant enzyme K297E, at pH 7.5 and 22°C | Escherichia coli | |
31 | - |
L-proline | mutant enzyme K297E/E303K, at pH 7.5 and 22°C | Escherichia coli | |
71 | - |
L-proline | wild type enzyme, at pH 7.5 and 22°C | Escherichia coli |