Protein Variants | Comment | Organism |
---|---|---|
additional information | C-terminal deletion mutant lacking last 50 amino acids, little effect on kinetic parameters | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
tRNAPro | pH 7.0, 55°C, C-terminal deletion mutant | Methanocaldococcus jannaschii | |
0.0022 | - |
tRNAPro | pH 7.0, 55°C, wild-type | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53300 | - |
2 * 53300, calcultaed | Methanocaldococcus jannaschii |
55700 | - |
2 * 55700, calculated | Methanothermobacter thermautotrophicus |
105000 | - |
gel filtration | Methanocaldococcus jannaschii |
113000 | - |
gel filtration | Methanothermobacter thermautotrophicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Methanothermobacter thermautotrophicus | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-proline + tRNAPro = AMP + diphosphate + L-prolyl-tRNAPro | one tRNA binds per dimeric enzyme, only 50% of active sites are active | Methanothermobacter thermautotrophicus | |
ATP + L-proline + tRNAPro = AMP + diphosphate + L-prolyl-tRNAPro | one tRNA binds per dimeric enzyme, only 50% of active sites are active | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-proline + tRNAPro | - |
Methanothermobacter thermautotrophicus | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | - |
Methanocaldococcus jannaschii | AMP + diphosphate + L-prolyl-tRNAPro | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 53300, calcultaed | Methanocaldococcus jannaschii |
dimer | 2 * 55700, calculated | Methanothermobacter thermautotrophicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
tRNAPro | pH 7.0, 55°C, C-terminal deletion mutant | Methanocaldococcus jannaschii | |
1.6 | - |
tRNAPro | pH 7.0, 55°C, wild-type | Methanocaldococcus jannaschii |