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6.25 - 55
2-selenaproline
-
0.28 - 2.2
3,4-dehydro-DL-proline
66
4-thiaproline
-
ATP-diphosphate exchange
2 - 7.1
cis(exo)-3,4-Methano-L-proline
53 - 55
cis-4-hydroxyproline
12.5
gamma-thiaproline
-
ATP-diphosphate exchange
1.43 - 5.3
L-azetidine-2-carboxylic acid
20 - 50
L-thiazolidine-4-carboxylic acid
-
ATP-diphosphate exchange
68
N-Methyl-L-alanine
-
ATP-diphosphate exchange
20
thiazolidine-4-carboxylic acid
-
ATP-diphosphate exchange
2 - 37
trans-4-hydroxyproline
-
pH 7.0, 37°C, recombinant wild-type enzyme, amino acid activation
0.00003 - 0.01414
tRNAPro
additional information
additional information
-
6.25
2-selenaproline
-
ATP-diphosphate exchange
-
55
2-selenaproline
-
ATP-diphosphate exchange
-
0.28
3,4-dehydro-DL-proline
-
ATP-diphosphate exchange
0.364
3,4-dehydro-DL-proline
-
ATP-diphosphate exchange
0.5
3,4-dehydro-DL-proline
-
ATP-diphosphate exchange
0.74
3,4-dehydro-DL-proline
-
ATP-diphosphate exchange
0.78
3,4-dehydro-DL-proline
-
ATP-diphosphate exchange
2.2
3,4-dehydro-DL-proline
-
ATP-diphosphate exchange
2.2
3,4-dehydro-DL-proline
-
ATP-diphosphate exchange, L-azetidine-2-carboxylic acid
1.4
3-thiaproline
-
ATP-diphosphate exchange
1.4
3-thiaproline
-
3,4-dehydro-DL-proline, , ATP-diphosphate exchange
0.049
ATP
aminoacylation reaction with L-proline, recombinant wild-type enzyme, pH 7.0, 70°C
0.06
ATP
aminoacylation reaction with L-cysteine, recombinant wild-type enzyme, pH 7.0, 70°C
2 - 3
cis(exo)-3,4-Methano-L-proline
-
ATP-diphosphate exchange
4.6
cis(exo)-3,4-Methano-L-proline
-
ATP-diphosphate exchange
7.1
cis(exo)-3,4-Methano-L-proline
-
ATP-diphosphate exchange
53
cis-4-hydroxyproline
-
pH 7.0, 37°C, recombinant mutant EcDELTAINS Gly12Ser4, amino acid activation
55
cis-4-hydroxyproline
-
pH 7.0, 37°C, recombinant wild-type enzyme, amino acid activation
79
L-alanine
-
37°C
140
L-alanine
-
pH 7.0, 37°C, recombinant wild-type enzyme, amino acid activation
454
L-alanine
mutant enzyme G217A, at pH 7.0 and 22°C
685
L-alanine
wild type enzyme, at pH 7.0 and 22°C
1360
L-alanine
mutant enzyme E218A, at pH 7.0 and 22°C
1.43
L-azetidine-2-carboxylic acid
-
ATP-diphosphate exchange
2
L-azetidine-2-carboxylic acid
-
ATP-diphosphate exchange
5.3
L-azetidine-2-carboxylic acid
-
ATP-diphosphate exchange
0.01
L-cysteine
-
recombinant enzyme, pH 7.2, 37°C
0.01
L-cysteine
-
recombinant enzyme, pH 7.2, 37°C
0.02
L-cysteine
-
recombinant enzyme, pH 7.2, 60°C
0.022
L-cysteine
aminoacylation reaction, recombinant wild-type enzyme, pH 7.0, 70°C
0.03
L-cysteine
-
recombinant enzyme, pH 7.2, 37°C
0.05
L-cysteine
-
ATP-diphosphate exchange, pH 7.5, 35°C
0.05
L-cysteine
-
recombinant enzyme, pH 7.2, 60°C
0.05
L-cysteine
-
recombinant enzyme, pH 7.2, 60°C
0.0745
L-cysteine
pH 7.0, 37°C
0.09
L-cysteine
-
ATP-diphosphate exchange reaction, pH 7.5, 35°C
0.09
L-cysteine
-
recombinant enzyme, pH 7.2, 60°C
0.17
L-cysteine
-
recombinant enzyme, pH 7.2, 37°C
0.17
L-cysteine
-
recombinant enzyme, pH 7.2, 37°C
0.18
L-cysteine
-
recombinant enzyme, pH 7.2, 37°C
0.19
L-cysteine
-
recombinant enzyme, pH 7.2, 37°C
0.2
L-cysteine
-
recombinant enzyme, pH 7.2, 37°C
0.26
L-cysteine
-
recombinant enzyme, pH 7.2, 37°C
0.137
L-Pro
-
ATP-diphosphate exchange
0.182
L-Pro
-
ATP-diphosphate exchange
0.29
L-Pro
-
ATP-diphosphate exchange
0.43
L-Pro
-
ATP-diphosphate exchange
0.45
L-Pro
-
ATP-diphosphate exchange
0.45
L-Pro
-
ATP-diphosphate exchange
0.625
L-Pro
-
ATP-diphosphate exchange
0.0046
L-proline
pH 7.0, 37°C
0.012
L-proline
-
60°C, pH not specified in the publication, ATP-diphosphate exchange assay
0.027
L-proline
aminoacylation reaction, recombinant wild-type enzyme, pH 7.0, 70°C
0.05
L-proline
-
recombinant enzyme, pH 7.2, 37°C
0.06
L-proline
-
recombinant enzyme, pH 7.2, 60°C
0.06
L-proline
-
isozyme ProRS-Cyt, at pH 7.2 and 30°C
0.061
L-proline
-
presence of bovine serum albumine and leucyl-tRNA synthetase, 50°C, pH 7.5
0.067
L-proline
-
ATP-diphosphate exchange reaction, pH 7.5, 35°C
0.067
L-proline
-
presence of bovine serum albumine, 50°C, pH 7.5
0.08
L-proline
-
recombinant enzyme, pH 7.2, 37°C
0.1
L-proline
-
isozyme ProRS-Org, at pH 7.2 and 30°C
0.11
L-proline
-
recombinant enzyme, pH 7.2, 37°C
0.14
L-proline
-
recombinant enzyme, pH 7.2, 37°C
0.14
L-proline
-
recombinant enzyme, pH 7.2, 37°C
0.15
L-proline
-
recombinant enzyme, pH 7.2, 60°C
0.15
L-proline
-
pH 7.5, 37°C, mutant K279A
0.16
L-proline
-
recombinant enzyme, pH 7.2, 37°C
0.17
L-proline
-
recombinant enzyme, pH 7.2, 37°C
0.18
L-proline
-
wild type enzyme, at pH 7.5 and 22°C
0.18
L-proline
-
wild type enzyme, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C
0.19
L-proline
-
mutant enzyme K297E/E303K, at pH 7.5 and 22°C
0.199
L-proline
-
mutant enzyme E303D, at pH 7.5 and 22°C
0.216
L-proline
-
mutant enzyme E303A, at pH 7.5 and 22°C
0.22
L-proline
-
mutant enzyme H302A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C
0.22
L-proline
-
mutant enzyme K297E, at pH 7.5 and 22°C
0.228
L-proline
wild type enzyme, at pH 7.0 and 22°C
0.25
L-proline
-
pH 7.0, 37°C, recombinant wild-type enzyme, amino acid activation
0.26
L-proline
-
recombinant enzyme, pH 7.2, 60°C
0.28
L-proline
-
recombinant enzyme, pH 7.2, 37°C
0.28
L-proline
-
recombinant enzyme, pH 7.2, 60°C
0.285
L-proline
-
ATP-diphosphate exchange reaction, pH 7.5, 35°C
0.29
L-proline
-
recombinant enzyme, pH 7.2, 37°C
0.3
L-proline
-
mutant enzyme E303K, at pH 7.5 and 22°C
0.3
L-proline
-
mutant enzyme G412A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C
0.33
L-proline
-
mutant enzyme D198A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C
0.427
L-proline
mutant enzyme G217A, at pH 7.0 and 22°C
0.45
L-proline
-
mutant enzyme N305A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C
0.62
L-proline
-
mutant enzyme H302A/G412A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C
0.76
L-proline
-
mutant enzyme F415A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C
1.03
L-proline
-
mutant enzyme E234A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C
3.4
L-proline
mutant enzyme E218A, at pH 7.0 and 22°C
3.4
L-proline
-
mutant enzyme E218A, in 50 mM HEPES (pH 7.5), 20 mM KCl, 25 mM MgCl2, at 37°C
31
L-proline
-
pH 7.5, 37°C, mutant K279A
50
L-proline
-
pH 7.0, 37°C, recombinant mutant EcDELTAINS Gly12Ser4, amino acid activation
45
N-methylglycine
-
ATP-diphosphate exchange
67
N-methylglycine
-
ATP-diphosphate exchange
100
N-methylglycine
-
ATP-diphosphate exchange
140
N-methylglycine
-
ATP-diphosphate exchange
300
N-methylglycine
-
approximate value, , ATP-diphosphate exchange
0.12
Pro
-
-
0.4
Pro
-
ATP-diphosphate exchange
0.8
Pro
-
3-thiaproline, , ATP-diphosphate exchange
0.00003
tRNAPro
pH 7.0, 37°C
0.000131
tRNAPro
-
pH 7.0, 37°C, recombinant mutant EcDELTAINS Gly12Ser4, aminoacylation
0.0014
tRNAPro
-
presence of bovine serum albumine and leucyl-tRNA synthetase, 50°C, pH 7.5
0.002
tRNAPro
-
pH 7.0, 55°C, C-terminal deletion mutant
0.0022
tRNAPro
-
pH 7.0, 55°C, wild-type
0.0022
tRNAPro
-
presence of leucyl-tRNA synthetase, 50°C, pH 7.5
0.0024
tRNAPro
-
presence of bovine serum albumine, 50°C, pH 7.5
0.0041
tRNAPro
-
50°C, pH 7.5
0.01414
tRNAPro
-
pH 7.0, 37°C, recombinant wild-type enzyme, aminoacylation
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
ProRS and leucyl-tRNA synthetase, LeuRS, interaction kinetics
-
additional information
additional information
-
single turnover and burst kinetics, steady-state kinetics, recombinant His-tagged enzyme, overview
-
additional information
additional information
-
wild-type and mutant enzyme kinetics
-