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4.4.1.1: cystathionine gamma-lyase

This is an abbreviated version!
For detailed information about cystathionine gamma-lyase, go to the full flat file.

Word Map on EC 4.4.1.1

Reaction

2-aminobut-2-enoate
=
2-iminobutanoate

Synonyms

AFUA_8G04340, C-S-lyase, C-S-lyase1, C-S-lyase2, C-S-lyase3, C-S-lyase4, cgl, CGL like protein, CS-LIKE, CSE, CSEgamma, CTH, CTT, cystalysin, cystathionase, cystathioninase, cystathionine beta/gamma-lyase, cystathionine gamma lyase, cystathionine gamma-lyase, cystathionine gamma-lyase-like protein, cystathionine-gamma-lyase, cysteine desulfhydrase, cysteine lyase, cystine desulfhydrase, dehydratase, homoserine, DES1, desulfhydrase, cysteine, EC 4.2.1.15, gamma-CTL, gamma-CTLase, gamma-cystathionase, hCSE, homoserine deaminase, homoserine deaminase-cystathionase, homoserine dehydratase, human cystathionine-gamma-lyase, L-Cys desulfhydrase, L-cysteine desulfhydrase, L-cysteine desulphydrase, L-cysteine-desulfhydrase, LCD, lyase, cystathionine gamma-, PRB-RA, Probasin-related antigen, TGME49_112930, XometC, yCGL

ECTree

     4 Lyases
         4.4 Carbon-sulfur lyases
             4.4.1 Carbon-sulfur lyases (only sub-subclass identified to date)
                4.4.1.1 cystathionine gamma-lyase

Cloned

Cloned on EC 4.4.1.1 - cystathionine gamma-lyase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme expressionin HEK-293 cells, Hep-G2 cells and IMR-90 cells
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli XL-1 cells
expression in Escherichia coli
expression in Escherichia coli strain Sure R
-
gene cys-16+, DNA and amino acid sequence determination and analysis, gene cys-16+ gene encodes a polypeptide of 417 amino acids with a highly conserved pyridoxal 5'-phosphate binding site and substrate-cofactor binding pocket
gene LmjF35.3230 or Lmj_CGL, functional complementation of a CGL null mutant strain DELTA CYS3 of Saccharomyces cerevisiae
overexpression in HEK-293, overexpression inhibits cell proliferation and DNA synthesis
quantitative enzyme expression PCR analysis
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Codon Plus using expression vector pGEX-4T3/GST-CSE
-
semi-quantitative reverse transcription-PCR analysis
site directed mutagenesis mutants, expression in Escherichia coli
-
the His-tagged enzyme is expressed in Escherichia coli KS1000 cells
-
transfection into HEK-293 cells
use of Escherichia coli codon-optimized gene
Y64A mutant
-