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4.4.1.1: cystathionine gamma-lyase

This is an abbreviated version!
For detailed information about cystathionine gamma-lyase, go to the full flat file.

Word Map on EC 4.4.1.1

Reaction

2-aminobut-2-enoate
=
2-iminobutanoate

Synonyms

AFUA_8G04340, C-S-lyase, C-S-lyase1, C-S-lyase2, C-S-lyase3, C-S-lyase4, cgl, CGL like protein, CS-LIKE, CSE, CSEgamma, CTH, CTT, cystalysin, cystathionase, cystathioninase, cystathionine beta/gamma-lyase, cystathionine gamma lyase, cystathionine gamma-lyase, cystathionine gamma-lyase-like protein, cystathionine-gamma-lyase, cysteine desulfhydrase, cysteine lyase, cystine desulfhydrase, dehydratase, homoserine, DES1, desulfhydrase, cysteine, EC 4.2.1.15, gamma-CTL, gamma-CTLase, gamma-cystathionase, hCSE, homoserine deaminase, homoserine deaminase-cystathionase, homoserine dehydratase, human cystathionine-gamma-lyase, L-Cys desulfhydrase, L-cysteine desulfhydrase, L-cysteine desulphydrase, L-cysteine-desulfhydrase, LCD, lyase, cystathionine gamma-, PRB-RA, Probasin-related antigen, TGME49_112930, XometC, yCGL

ECTree

     4 Lyases
         4.4 Carbon-sulfur lyases
             4.4.1 Carbon-sulfur lyases (only sub-subclass identified to date)
                4.4.1.1 cystathionine gamma-lyase

Crystallization

Crystallization on EC 4.4.1.1 - cystathionine gamma-lyase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
molecular modeling and dynamics simulations. Enzyme has high conformational stability with high flexibility at the nonstructural regions
structures of mutant E59N/R119L/E339V with distinct reaction intermediates, including internal aldimine, substrate-bound, gem-diamine, and external aldimine forms. A locally unfolded active site correlates with inhibition of activity as a function of ionic strength
using sitting drop and hanging drop vapor diffusion methods at room temperature
-
molecular modeling of the external aldimine of wild-type in complex with L-cystathionine
crystals of two different shapes, plate-shaped and pyramid-shaped, diffract to 2.9 and 3.2 A resolution and belong to the primitive orthogonal space group P212121 and the tetragonal space group P41 (or P43), with unit-cell parameters a = 73.0, b = 144.9, c = 152.3 A and a = b = 78.2, c = 300.7 A, respectively. For the P212121 crystals, three or four monomers exist in the asymmetric unit with a corresponding VM of 3.02 or 2.26 A3/Da and a solvent content of 59.3 or 45.7%. For the P41 (or P43) crystals, four or five monomers exist in the asymmetric unit with a corresponding VM of 2.59 or 2.09 A3/Da and a solvent content of 52.5 or 40.6%