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4.3.3.7: 4-hydroxy-tetrahydrodipicolinate synthase

This is an abbreviated version!
For detailed information about 4-hydroxy-tetrahydrodipicolinate synthase, go to the full flat file.

Word Map on EC 4.3.3.7

Reaction

pyruvate
+
L-aspartate-4-semialdehyde
=
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate
 +
H2O

Synonyms

Aq_1143, AT2G45440, BA3935 gene product, cDHDPS, CjDHDPS, DapA, DapA2, DHDPA synthase, DHDPS, DHDPS2, dihydro-dipicolinic acid synthase, dihydrodipicolinate synthase, dihydrodipicolinic acid synthase, dihydrodipocolinate synthase, dihydropicolinate synthetase, EC 4.2.1.52, FaDHDPS, HTPA synthase, More, MosA, MosA protein, MRSA-DHDPS, PA1010, pyruvate-aspartic semialdehyde condensing enzyme, Rv2753c, synthase, dihydrodipicolinate, VEG81, Vegetative protein 81

ECTree

     4 Lyases
         4.3 Carbon-nitrogen lyases
             4.3.3 Amine-lyases
                4.3.3.7 4-hydroxy-tetrahydrodipicolinate synthase

Cloned

Cloned on EC 4.3.3.7 - 4-hydroxy-tetrahydrodipicolinate synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
amplified product cloned into a pCR-Blunt IITOP vector, producing the construct pNS01. DHDPS gene then subcloned into the Escherichia coli expression vector Champion pET101/D-TOPO to produce pNS02. Native recombinant protein overexpressed in Escherichia coli BL21 (DE3)
-
as His-tagged constructs
-
dapA gene (Rv2753c) is cloned and expressed in Escherichia coli
dapA gene encoding DHDPS amplified and cloned into the pET11a expression vector, expressed in Escherichia coli BL21-DE3
Q81WN7
DHDPS mutants expressed in Escherichia coli strain AT997r-
DHDPS overexpressed in Escherichia coli AT997recA-, transformed with site-directed mutants based on the pBluescript plasmid pJG001
DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain BL21(DE3)
-
expressed in Escherichia coli BL21 (DE3), pET11a expression vector
expressed in Escherichia coli BL21(DE3), full length dapA gene (DHDPS-FL) and C-terminal variant (DHDPS D228-295) lacking the three helical domains, pET22b vector
expressed in Escherichia coli BL21(DE3), pET24d vector
expressed in Escherichia coli STBL2, pET23b vector
expressed in Escherichia coli XL-1 Blue harbouring the plasmid pJG001
expressed in Escherichia coli XL1-blue, pBluescript vector, recombinant protein
expressed in Escherichia coli, BL21 (DE3) strain carrying the Cbot-DHDPS gene (DapA)
-
expressed in Escherichia coli, pJG001 plasmid for site-directed mutagenesis, mutant Y107W expressed in dapA-negative strain AT997r-
expression in Escherichia coli
expression in Escherichia coli as His-tagged construct
expression in Nicotina tabacum
-
expression in Solanum tuberosum
-
expression of N-terminally His6-tagged enzyme with a thrombin cleavage site in Escherichia coli strain BL21 (DE3)
-
from pETSA1, overexpressed
-
gene dapA or Aq_1143, sequence comparisons, recombinant enzyme expression in Escherichia coli strain BL21-CodonPlus (DE3)-RIL
gene dapA, DNA and amino acid sequence determination and analysis, expression of His-tagged DHDPS in Escherichia coli strain BL21(DE3)
gene dapA, expression of His-tagged DHDPS in Escherichia coli strain BL21(DE3)
-
gene dapA, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene dapA, expression of His-tagged enzyme in Escherichia coli strain XL1-Blue
-
gene dapA, expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene dapA, expression of wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3)
-
gene dapA, overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 Star (DE3)
-
gene dapA, recombinant expression of lysine-insensitive mutants in Escherichia coli strain MG1655 with a yield improved by 46% compared to the wild-type enzyme
-
gene dapA, sequence comparison with Escherichia coli enzyme, expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3)
gene dapA1, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3)
gene dapA10, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3)
gene dapA2, sequence comparisons, expression of the His-tagged isozyme in Escherichia coli strain BL21(DE3)
gene dapA3, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3)
gene dapA4, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3)
gene dapA5, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3)
gene dapA6, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3)
gene dapA7, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3)
gene dapA8, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3)
gene dapA9, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3)
inserted into the vector pET11a and transformed into Escherichia coli strain CodonPlus BL21 (DE3)
-
into pBI121 vector in order to obtain fusion construct of the DHDPS sequence with the reporter gene GUS transiently expressed in the onion epidermal cells by particle gun-mediated bombardment. DHDPS cDNA coding sequence subcloned into the pUC18 plasmid and expressed in Escherichia coli strain AT997, an auxotroph lacking DHDPS activity
into the expression vector pET151-D to give the plasmid pFH02, to transform chemically super-competent Escherichia coli Top10 cells
into the pET-21a expression vector and introduced into Escherichia coli Rosetta (DE3) strain
isozymes MtDHDPS, MtDHDPS2, MtDHDPS3, and MtDHDPS4, phylogenetic analysis, quantitative RT-PCR expression analysis, the different isogenes are transcriptionally regulated in an organ-specific manner, subcloning in Escherichia coli strain DH5alpha, expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), coexpresion of His-tagged isozyme MtDHDPS3 with N-terminally FLAG-tagged enzyme AtDHDPS2 from Arabidopsis thliana in Escherichia coli strain BL21(DE3), expression of isozyme MtDHDPS3 in transgenic Arabidopsis thaliana plants under the control of a constitutive 35S promoter via Agrobacterium tumefaciens transfection resulting in an inhibition of enzyme activity
-
ligated into the expression vector pET30a and transformed into the Escherichia coli strain B834 for overexpression
mutant T44S expressed in dapA-deficient Escherichia coli AT997r-
orf AT2G45440, recombinant His-tagged enzyme expression in Escherichia coli strain BL21 (DE3) Star
overexpression of wild-type and mutant enzymes in strain XL1-Blue
PCR product ligated into pCRBluntII-TOPO and transformed into Escherichia coli One Shot TOP10. The dapA insert then amplified, the product, which now contains NdeI and BamHI restriction-endonuclease sites, ligated into pCR-BluntII-TOPO and transformed into Escherichia coli One Shot TOP10, which enables efficient ligation into an expression vector. DapA and linearized pET-11a ligated with T4 DNA ligase and transformed into Escherichia coli BL21(DE3)
Q81WN7
plasmid pB3935 containing a His-tagged copy of the dapA2 gene and a gene conferring kanamycin resistance, transformed into Escherichia coli BL21(DE3) for protein overexpression
Q81WN7
recombinant enzyme expression in Escherichia coli strain XL-1 Blue using plasmid pJG001
recombinant His-tagged protein is expressed in Escherichia coli BL-21(DE3)
-
the dapA and dapA-H225* genes introduced into the vector pUCX, to transform the auxotrophic Escherichia coli XL1-Blue KanRDELTAdapA cells, under the control of a lac promoter/repressor system. Mutations in dapA introduced into the pET-151/D-TOPO plasmid. C-terminal truncated DHDPS (H225*) expressed in Escherichia coli BL21 Star (DE3)
the dapA open reading frame is cloned in the expression vector pET28a for expression as N-terminal 6 x His-tagged protein
wild-type and mutant cloned into plasmid pET-151/D-TOPO and expressed in Escherichia coli BL21 Star (DE3) competent cells