Cloned (Comment) | Organism |
---|---|
inserted into the vector pET11a and transformed into Escherichia coli strain CodonPlus BL21 (DE3) | Clostridium botulinum |
Crystallization (Comment) | Organism |
---|---|
in the presence of its substrate pyruvate, by the sitting-drop vapour diffusion method, to 1.2 A resolution. Belongs to space group C2, in contrast to the unbound form, which has trigonal symmetry. Unit-cell parameters are a = 143.4, b = 54.8, c = 94.3 A , beta = 126.3. The crystal volume per protein weight is 2.3 A3 Da-1 (based on the presence of two monomers in the asymmetric unit), with an estimated solvent content of 46% | Clostridium botulinum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31530 | - |
purified enzyme, mass spectrometry | Clostridium botulinum |
31650 | - |
mass spectrometry | Clostridium botulinum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium botulinum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by anion-exchange chromatography, hydrophobic interaction and gel filtration | Clostridium botulinum |
Storage Stability | Organism |
---|---|
-20°C, 20 mM Tris-HCl buffer, pH 8.0 | Clostridium botulinum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate 4-semialdehyde + pyruvate | - |
Clostridium botulinum | dihydrodipicolinate + 2 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | crystallography | Clostridium botulinum |
Synonyms | Comment | Organism |
---|---|---|
DapA | - |
Clostridium botulinum |
DHDPS | - |
Clostridium botulinum |
dihydrodipicolinate synthase | - |
Clostridium botulinum |