Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-methionine | at 10 mM 112% activity, at 100 mM 111% activity | Escherichia coli | |
meso-diaminopimelate | at 1 mM 108% activity, at 10 mM 122% activity | Escherichia coli | |
additional information | meso-diaminopimelate has no effect | Bacillus anthracis |
Application | Comment | Organism |
---|---|---|
drug development | DHDPS is a potential antibiotic target | Bacillus anthracis |
Cloned (Comment) | Organism |
---|---|
plasmid pB3935 containing a His-tagged copy of the dapA2 gene and a gene conferring kanamycin resistance, transformed into Escherichia coli BL21(DE3) for protein overexpression | Bacillus anthracis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-aspartate 4-semialdehyde | DHDPS is subject to substrate inhibition by L-aspartate 4-semialdehyde | Bacillus anthracis | |
L-lysine | at 1 mM 23% residual activity, at 100 mM 13% residual activity | Escherichia coli | |
L-methionine | at 10 or 100 mM 80% residual activity | Bacillus anthracis | |
L-threonine | at 100 mM 40% residual activity | Bacillus anthracis | |
L-threonine | at 100 mM 23% residual activity, at 100 mM 33% residual activity | Escherichia coli | |
additional information | is not inhibited by 1-100 mM L-lysine or L-isoleucine | Bacillus anthracis | |
additional information | 1-100 mM L-isoleucine has no effect | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
L-aspartate 4-semialdehyde | - |
Bacillus anthracis | |
0.43 | - |
pyruvate | - |
Bacillus anthracis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32290 | - |
mass spectrometry | Bacillus anthracis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus anthracis | Q81WN7 | - |
- |
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity | Bacillus anthracis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate 4-semialdehyde + pyruvate | active sites are similiar to Bacillus anthracis DHDPS | Escherichia coli | dihydrodipicolinate + 2 H2O | - |
? | |
L-aspartate 4-semialdehyde + pyruvate | active sites are similiar to Escherichia coli DHDPS | Bacillus anthracis | dihydrodipicolinate + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DapA2 | - |
Bacillus anthracis |
DHDPS | - |
Escherichia coli |
DHDPS | - |
Bacillus anthracis |
dihydrodipicolinate synthase | - |
Escherichia coli |
dihydrodipicolinate synthase | - |
Bacillus anthracis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
59.8 | - |
is thermally stabilised by the first substrate, pyruvate, to a greater extent than its Escherichia coli counterpart | Bacillus anthracis |
61.3 | - |
is thermally stabilised by the first substrate, pyruvate | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
76 | - |
pyruvate | - |
Bacillus anthracis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
5.4 | - |
L-aspartate 4-semialdehyde | - |
Bacillus anthracis |