Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.3.3.7 extracted from

  • Domigan, L.J.; Scally, S.W.; Fogg, M.J.; Hutton, C.A.; Perugini, M.A.; Dobson, R.C.; Muscroft-Taylor, A.C.; Gerrard, J.A.; Devenish, S.R.
    Characterisation of dihydrodipicolinate synthase (DHDPS) from Bacillus anthracis (2009), Biochim. Biophys. Acta, 1794, 1510-1516.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
L-methionine at 10 mM 112% activity, at 100 mM 111% activity Escherichia coli
meso-diaminopimelate at 1 mM 108% activity, at 10 mM 122% activity Escherichia coli
additional information meso-diaminopimelate has no effect Bacillus anthracis

Application

Application Comment Organism
drug development DHDPS is a potential antibiotic target Bacillus anthracis

Cloned(Commentary)

Cloned (Comment) Organism
plasmid pB3935 containing a His-tagged copy of the dapA2 gene and a gene conferring kanamycin resistance, transformed into Escherichia coli BL21(DE3) for protein overexpression Bacillus anthracis

Inhibitors

Inhibitors Comment Organism Structure
L-aspartate 4-semialdehyde DHDPS is subject to substrate inhibition by L-aspartate 4-semialdehyde Bacillus anthracis
L-lysine at 1 mM 23% residual activity, at 100 mM 13% residual activity Escherichia coli
L-methionine at 10 or 100 mM 80% residual activity Bacillus anthracis
L-threonine at 100 mM 40% residual activity Bacillus anthracis
L-threonine at 100 mM 23% residual activity, at 100 mM 33% residual activity Escherichia coli
additional information is not inhibited by 1-100 mM L-lysine or L-isoleucine Bacillus anthracis
additional information 1-100 mM L-isoleucine has no effect Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
L-aspartate 4-semialdehyde
-
Bacillus anthracis
0.43
-
pyruvate
-
Bacillus anthracis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32290
-
mass spectrometry Bacillus anthracis

Organism

Organism UniProt Comment Textmining
Bacillus anthracis Q81WN7
-
-
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Bacillus anthracis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-aspartate 4-semialdehyde + pyruvate active sites are similiar to Bacillus anthracis DHDPS Escherichia coli dihydrodipicolinate + 2 H2O
-
?
L-aspartate 4-semialdehyde + pyruvate active sites are similiar to Escherichia coli DHDPS Bacillus anthracis dihydrodipicolinate + 2 H2O
-
?

Synonyms

Synonyms Comment Organism
DapA2
-
Bacillus anthracis
DHDPS
-
Escherichia coli
DHDPS
-
Bacillus anthracis
dihydrodipicolinate synthase
-
Escherichia coli
dihydrodipicolinate synthase
-
Bacillus anthracis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
59.8
-
is thermally stabilised by the first substrate, pyruvate, to a greater extent than its Escherichia coli counterpart Bacillus anthracis
61.3
-
is thermally stabilised by the first substrate, pyruvate Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
76
-
pyruvate
-
Bacillus anthracis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5.4
-
L-aspartate 4-semialdehyde
-
Bacillus anthracis