Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a bona fide drug target for treatment of the Crown Gall disease on crops caused by Agrobacterium tumefaciens, rational design of pesticide agents | Agrobacterium tumefaciens |
Cloned (Comment) | Organism |
---|---|
gene dapA1, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) | Agrobacterium tumefaciens |
gene dapA10, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) | Agrobacterium tumefaciens |
gene dapA2, sequence comparisons, expression of the His-tagged isozyme in Escherichia coli strain BL21(DE3) | Agrobacterium tumefaciens |
gene dapA3, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) | Agrobacterium tumefaciens |
gene dapA4, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) | Agrobacterium tumefaciens |
gene dapA5, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) | Agrobacterium tumefaciens |
gene dapA6, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) | Agrobacterium tumefaciens |
gene dapA7, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) | Agrobacterium tumefaciens |
gene dapA8, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) | Agrobacterium tumefaciens |
gene dapA9, sequence comparisons, expression of the His-tagged enzyme isozyme in Escherichia coli strain BL21(DE3) | Agrobacterium tumefaciens |
Crystallization (Comment) | Organism |
---|---|
crystal structure analysis, PDB ID 2HMC, structure comparisons | Agrobacterium tumefaciens |
crystal structure analysis, PDB ID 2R8Wm, structure comparisons | Agrobacterium tumefaciens |
crystal structure analysis, PDB ID 3B4U, structure comparisons | Agrobacterium tumefaciens |
crystal structure analysis, PDB IDs 4I7U, 4I7V, and 4I7W, resolution at 1.42-1.69 A, structure comparisons | Agrobacterium tumefaciens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(S)-aspartate-4-semialdehyde | substrate inhibition | Agrobacterium tumefaciens | |
(S)-lysine | allosteric feedback inhibition; allosteric feedback inhibition; allosteric feedback inhibition; allosteric feedback inhibition; allosteric feedback inhibition; allosteric feedback inhibition; allosteric feedback inhibition, allosteric site modeling; allosteric feedback inhibition, allosteric site modeling; allosteric feedback inhibition, allosteric site modeling; allosteric feedback inhibition, allosteric site modeling | Agrobacterium tumefaciens | |
pyruvate | substrate inhibition | Agrobacterium tumefaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | 0.32 | (S)-aspartate-4-semialdehyde | pH and temperature not specified in the publication | Agrobacterium tumefaciens | |
0.31 | 0.44 | pyruvate | pH and temperature not specified in the publication | Agrobacterium tumefaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-aspartate-4-semialdehyde + pyruvate | Agrobacterium tumefaciens | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O | - |
? | |
(S)-aspartate-4-semialdehyde + pyruvate | Agrobacterium tumefaciens C58 / ATCC 33970 | - |
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | A9CFV4 | dapA10; gene dapA10 | - |
Agrobacterium tumefaciens | A9CGZ4 | dapA8; gene dapA8 | - |
Agrobacterium tumefaciens | A9CHR2 | dapA5; gene dapA5 | - |
Agrobacterium tumefaciens | A9CL94 | dapA2; genes dapA2 | - |
Agrobacterium tumefaciens | A9CL97 | dapA3; gene dapA3 | - |
Agrobacterium tumefaciens | Q7CU96 | dapA9; gene dapA9 | - |
Agrobacterium tumefaciens | Q7D0E8 | dapA6; gene dapA6 | - |
Agrobacterium tumefaciens | Q7D313 | dapA1; gene dapA1 | - |
Agrobacterium tumefaciens | Q7D3Z9 | dapA4; gene dapA4 | - |
Agrobacterium tumefaciens | Q8UGL3 | dapA7; gene dapA7 | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | A9CFV4 | dapA10; gene dapA10 | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | A9CGZ4 | dapA8; gene dapA8 | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | A9CHR2 | dapA5; gene dapA5 | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | A9CL94 | dapA2; genes dapA2 | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | A9CL97 | dapA3; gene dapA3 | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | Q7CU96 | dapA9; gene dapA9 | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | Q7D0E8 | dapA6; gene dapA6 | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | Q7D313 | dapA1; gene dapA1 | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | Q7D3Z9 | dapA4; gene dapA4 | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | Q8UGL3 | dapA7; gene dapA7 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged isozyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography | Agrobacterium tumefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-aspartate-4-semialdehyde + pyruvate | - |
Agrobacterium tumefaciens | (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O | - |
? | |
(S)-aspartate-4-semialdehyde + pyruvate | bi-bi ping-pong substrate model | Agrobacterium tumefaciens | (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O | - |
? | |
(S)-aspartate-4-semialdehyde + pyruvate | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O | - |
? | |
(S)-aspartate-4-semialdehyde + pyruvate | bi-bi ping-pong substrate model | Agrobacterium tumefaciens C58 / ATCC 33970 | (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | the enzyme adopts the canonical homotetrameric structure in both solution and the crystal state | Agrobacterium tumefaciens |
Synonyms | Comment | Organism |
---|---|---|
DHDPS | - |
Agrobacterium tumefaciens |
dihydrodipicolinate synthase | - |
Agrobacterium tumefaciens |
HTPA synthase | UniProt | Agrobacterium tumefaciens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
(S)-aspartate-4-semialdehyde | pH and temperature not specified in the publication | Agrobacterium tumefaciens | |
0.19 | 0.57 | pyruvate | pH and temperature not specified in the publication | Agrobacterium tumefaciens |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyses the first committed step in the lysine biosynthesis pathway, the condensation of pyruvate and (S)-aspartate semialdehyde to form (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid | Agrobacterium tumefaciens |
additional information | the catalytic triad, consisting of Tyr133, Thr44, and Tyr107, acts as a proton relay to transfer protons to and from the active site via a water-filled channel leading to bulk solvent | Agrobacterium tumefaciens |