Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

4.3.3.7: 4-hydroxy-tetrahydrodipicolinate synthase

This is an abbreviated version!
For detailed information about 4-hydroxy-tetrahydrodipicolinate synthase, go to the full flat file.

Word Map on EC 4.3.3.7

Reaction

pyruvate
+
L-aspartate-4-semialdehyde
=
(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate
 +
H2O

Synonyms

Aq_1143, AT2G45440, BA3935 gene product, cDHDPS, CjDHDPS, DapA, DapA2, DHDPA synthase, DHDPS, DHDPS2, dihydro-dipicolinic acid synthase, dihydrodipicolinate synthase, dihydrodipicolinic acid synthase, dihydrodipocolinate synthase, dihydropicolinate synthetase, EC 4.2.1.52, FaDHDPS, HTPA synthase, More, MosA, MosA protein, MRSA-DHDPS, PA1010, pyruvate-aspartic semialdehyde condensing enzyme, Rv2753c, synthase, dihydrodipicolinate, VEG81, Vegetative protein 81

ECTree

     4 Lyases
         4.3 Carbon-nitrogen lyases
             4.3.3 Amine-lyases
                4.3.3.7 4-hydroxy-tetrahydrodipicolinate synthase

Temperature Stability

Temperature Stability on EC 4.3.3.7 - 4-hydroxy-tetrahydrodipicolinate synthase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 95
-
purified recombinnat wild-type enzyme
46.5
melting temperature in the absence of substrates
50 - 60
Q81WN7
thermostability is significantly enhanced in the presence of the substrate pyruvate
59.5
-
melting temperature in the absence of substrates
59.8
Q81WN7
is thermally stabilised by the first substrate, pyruvate, to a greater extent than its Escherichia coli counterpart
61.3
-
is thermally stabilised by the first substrate, pyruvate
65
the recombinant enzyme adopts a characteristic alpha/beta conformation which is retained up to 65°C
74 - 79
-
inactivation energy: 117000 cal/mol, change in free energy, DELTAF: 23400 cal/mol, change in enthalpy, DELTAH: 113500 cal/mol, change in entropy DELTAS: 259 cal/degre e/mol
75
-
pyruvate increases the half-life from 2.7 to 18.3 min
80
-
no reactivation after heating to 80°C, pyruvate increases thermostability