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Literature summary for 4.3.3.7 extracted from

  • Schnell, R.; Oehlmann, W.; Sandalova, T.; Braun, Y.; Huck, C.; Maringer, M.; Singh, M.; Schneider, G.
    Tetrahydrodipicolinate N-succinyltransferase and dihydrodipicolinate synthase from Pseudomonas aeruginosa: structure analysis and gene deletion (2012), PLoS ONE, 7, e31133.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.3.3.7

Application

Application Comment Organism
additional information the enzyme is not an optimal target for drug development against Pseudomonas aeruginosa Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment) Organism
gene dapA, expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant His6-tagged enzyme, hanging drop vapour diffusion method, mixing of 0.002 m of 12.5 mg/ml protein solution with 0.002 ml of reservoir solution containing 18% of PEG6000, 0.2 M MgCl2, and 0.1 M TRIS-HCl, pH 7.6, X-ray diffraction structure determination and analysis at 1.6 A resolution, molecular replacement Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
additional information mutant construction by gene replacement of gene dapA (PA1010) via the sacB-based strategy Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-aspartate-4-semialdehyde + pyruvate Pseudomonas aeruginosa
-
 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9I4W3 gene dapA or PA1010
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, the proteolytic cleavage by TEV protease does not remove the N-terminal His6-tag efficiently Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-aspartate-4-semialdehyde + pyruvate
-
 Pseudomonas aeruginosa (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O
-
 ?

Synonyms

Synonyms Comment Organism
DapA
-
 Pseudomonas aeruginosa
PA1010
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Pseudomonas aeruginosa

General Information

General Information Comment Organism
malfunction enzyme mutants with deleted dapA gene are viable and able to grow in a mouse lung infection model Pseudomonas aeruginosa
metabolism the enzyme catalyzes the first step in the diaminopimelic acid pathway of lysine biosynthesis Pseudomonas aeruginosa
additional information structure-based sequence alignments, based on the DapA crystal structure, reveal the presence of two homologues, PA0223 and PA4188, in Pseudomonas aeruginosa that can substitute for DapA in the PAO1DELTAdapA mutant. In vitro experiments using recombinant PA0223 protein do not detect any DapA activity Pseudomonas aeruginosa