Application | Comment | Organism |
---|---|---|
drug development | the intracellular enzyme dihydrodipicolinate synthase a potential drug target because it is essential for the growth of bacteria while it is absent in humans | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
gene dapA, DNA and amino acid sequence determination and analysis, expression of His-tagged DHDPS in Escherichia coli strain BL21(DE3) | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
purified recombinant DHDPS, free or in complex with inhibitor (S)-lysine, 15 mg/ml protein in 50 mM Tris-HCl, pH 8.5, at 20°C using hanging drop vapour diffusion method, mixing of 0.005 ml protein solution with 0.005 ml well solution containing 30% w/v PEG-3350, 170 mM MgCl2, 70 mM Tris-HCl, pH 8.5, and 6% v/v propylene glycol. Crystals obtained without 6% propylene glycol are soaked in the reservoir containing 20 mg/ml (S)-lysine, X-ray diffraction structure determination and analysis at 2.65-2.85 A resolution | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-lysine | binds at three sites to the enzyme, binding structure, overview | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
L-aspartate 4-semialdehyde | pH 8.0, 37°C, recombinant His-tagged enzyme | Pseudomonas aeruginosa | |
0.9 | - |
pyruvate | pH 8.0, 37°C, recombinant His-tagged enzyme | Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Pseudomonas aeruginosa | 5622 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate 4-semialdehyde + pyruvate | Pseudomonas aeruginosa | - |
(S)-2,3-dihydropyridine-2,6-dicarboxylate + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
no activity in Homo sapiens | - |
- |
- |
Pseudomonas aeruginosa | Q9I4W3 | gene dapA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged DHDPS from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate 4-semialdehyde + pyruvate | - |
Pseudomonas aeruginosa | (S)-2,3-dihydropyridine-2,6-dicarboxylate + 2 H2O | - |
? | |
additional information | the active site is formed by residues Thr44, Tyr107 and Tyr133, stereochemically suitable for catalytic function | Pseudomonas aeruginosa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | three-dimensional structure determination shows that DHDPS forms a homodimer which is stabilized by several hydrogen bonds and van der Waals forces at the interface, active site structure, overview. Each monomer is composed of two domains, the N-terminal domain consists of residues 1-224, and forms an 8-fold parallel alpha/beta barrel | Pseudomonas aeruginosa |
More | the recombinant enzyme adopts a characteristic alpha/beta conformation which is retained up to 65°C | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
DHDPS | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
the recombinant enzyme adopts a characteristic alpha/beta conformation which is retained up to 65°C | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas aeruginosa |