Literature summary for 4.3.3.7 extracted from

Atkinson, S.C.; Dogovski, C.; Newman, J.; Dobson, R.C.; Perugini, M.A.
Cloning, expression, purification and crystallization of dihydrodipicolinate synthase from the grapevine Vitis vinifera (2011), Acta Crystallogr. Sect. F, 67, 1537-1541.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of N-terminally His6-tagged enzyme with a thrombin cleavage site in Escherichia coli strain BL21 (DE3)	Vitis vinifera

Crystallization (Commentary)

Crystallization (Comment)	Organism
purifed recombinant detagged enzyme in complex with pyruvate, hanging drop vapour diffusion method, mixing of 0.002 ml of 10 mg/ml protein in 20 mM Tris, 150 mM NaCl, pH 8.0, and 20 mM pyruvate, with 0.002 ml of reservoir solution containing 0.1 M Bis-Tris propane, pH 8.2, 0.2 M sodium bromide, and 20% w/v PEG 3350, equilibration against 1 ml of reservoir solution, 20°C, method ooptimization, X-ray diffraction structure determination and analysis at 2.2 A resolution	Vitis vinifera

Crystallization (Comment)

Organism

purifed recombinant detagged enzyme in complex with pyruvate, hanging drop vapour diffusion method, mixing of 0.002 ml of 10 mg/ml protein in 20 mM Tris, 150 mM NaCl, pH 8.0, and 20 mM pyruvate, with 0.002 ml of reservoir solution containing 0.1 M Bis-Tris propane, pH 8.2, 0.2 M sodium bromide, and 20% w/v PEG 3350, equilibration against 1 ml of reservoir solution, 20°C, method ooptimization, X-ray diffraction structure determination and analysis at 2.2 A resolution

Vitis vinifera

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(S)-aspartate-4-semialdehyde + pyruvate	Vitis vinifera	-	(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Vitis vinifera	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography, tag cleavage by thrombin, and gel filtration	Vitis vinifera

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-aspartate-4-semialdehyde + pyruvate	-	Vitis vinifera	(2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid + H2O	-	?

Synonyms

Synonyms	Comment	Organism
DHDPS	-	Vitis vinifera
dihydrodipicolinate synthase	-	Vitis vinifera