EC Number   |
|---|
    4.3.3.7 | - |
| 4.3.3.7 | amplified product cloned into a pCR-Blunt IITOP vector, producing the construct pNS01. DHDPS gene then subcloned into the Escherichia coli expression vector Champion pET101/D-TOPO to produce pNS02. Native recombinant protein overexpressed in Escherichia coli BL21 (DE3) |
| 4.3.3.7 | as His-tagged constructs |
| 4.3.3.7 | dapA gene (Rv2753c) is cloned and expressed in Escherichia coli |
| 4.3.3.7 | dapA gene encoding DHDPS amplified and cloned into the pET11a expression vector, expressed in Escherichia coli BL21-DE3 |
| 4.3.3.7 | DHDPS mutants expressed in Escherichia coli strain AT997r- |
| 4.3.3.7 | DHDPS overexpressed in Escherichia coli AT997recA-, transformed with site-directed mutants based on the pBluescript plasmid pJG001 |
| 4.3.3.7 | DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain BL21(DE3) |
| 4.3.3.7 | expressed in Escherichia coli BL21 (DE3), pET11a expression vector |
| 4.3.3.7 | expressed in Escherichia coli BL21(DE3), full length dapA gene (DHDPS-FL) and C-terminal variant (DHDPS D228-295) lacking the three helical domains, pET22b vector |
