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4.1.3.27: anthranilate synthase

This is an abbreviated version!
For detailed information about anthranilate synthase, go to the full flat file.

Word Map on EC 4.1.3.27

Reaction

chorismate
+
L-glutamine
=
anthranilate
+
pyruvate
+
L-glutamate

Synonyms

ANTA synthase, anthranilate synthase, anthranilate synthase a1, anthranilate synthase alpha 1, anthranilate synthase alpha 2, anthranilate synthase alpha2, anthranilate synthase component I, anthranilate synthase-phosphoribosyltransferase, anthranilate synthetase, AS-I, ASA, ASA1, ASA2, ASalpha, ASalphabeta, ASalphabeta-1, ASB, ASB1, CaASA1, CaASA2, chorismate lyase, Glutamine amido-transferase, Glutamine amidotransferase, HvASa2, OASA, OASA1, OASA1D, OASA2, PhnAB, PhnAB anthranilate synthase, synthase, anthranilate, synthetase, anthranilate, TrpDE, TrpE, TrpED

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.3 Oxo-acid-lyases
                4.1.3.27 anthranilate synthase

Molecular Weight

Molecular Weight on EC 4.1.3.27 - anthranilate synthase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
122000
-
gel filtration
126000
-
sucrose density gradient centrifugation
139300
-
deduced from amino acid sequence
14000
-
1 * 14000 + 1 * 70000, gel filtration or sucrose density gradient centrifugation after separation of subunits
140000
-
sucrose density gradient centrifugation
141000
-
sucrose density gradient centrifugation
143000
-
gel filtration
150000
158000
-
gel filtration
16000
-
1 * 16000 + 1 * 80000
160000
18000
20000
-
2 * 20000 + 2 * 50000, Gln-dependent enzyme, composed of 2 NH4+-dependent subunits and of 2 amidotransferase subunits, I2II2, gel filtration
200000
20956
-
2 * 20956 + 2 * 60000
21000
-
ASI, ASII, 2 * 21000 + 2 * 60000, SDS-PAGE
21050
calculated, beta, PabA subunit
21100
1 * 57000 + 1 * 23000, SDS-PAGE, 1 * 48500 + 1 * 21100, deduced from gene sequence
21913
-
2 * 47743 + 2 * 21913, deduced from amino acid sequence
220000
-
gel filtration
23000
1 * 57000 + 1 * 23000, SDS-PAGE, 1 * 48500 + 1 * 21100, deduced from gene sequence
25500
-
2 * 25500 + 2 * 67000, SDS-PAGE
28000
-
1 * 28000 + 1 * 72000, gel filtration after separation of subunits
37000
-
1 * 37000 + 1 * 60000, isoenzyme AS-b, SDS-PAGE
400000
-
three-enzyme complex containing anthranilate synthetase, phosphoribosyl anthranilate isomerase and indole-3-glycerol phosphate synthetase, gel filtration
47743
-
2 * 47743 + 2 * 21913, deduced from amino acid sequence
47930
calculated, probable anthranilate synthase ORF CV0568
48500
1 * 57000 + 1 * 23000, SDS-PAGE, 1 * 48500 + 1 * 21100, deduced from gene sequence
50000
54020
calculated, alpha, TrpE subunit
57000
1 * 57000 + 1 * 23000, SDS-PAGE, 1 * 48500 + 1 * 21100, deduced from gene sequence
60000
62300
recombinant fusion protein, determined by SDS-PAGE
62350
determined by mass spectrometry
63400
-
1 * 18000 + 1 * 63400, sucrose density gradient centrifugation
64000
-
1 * 18000 + 1 * 64000
64512
x * 64512, calculation from nucleotide sequence
65013
x * 65013, calculation from nucleotide sequence
66000
-
gel filtration
67000
-
2 * 25500 + 2 * 67000, SDS-PAGE
70000
71000
72000
-
1 * 28000 + 1 * 72000, gel filtration after separation of subunits
76000
-
2 * 76000 + 2 * 84000, alpha2,beta2, SDS-PAGE
79000
-
1 * 79000, SDS-PAGE
80000
84000
-
2 * 76000 + 2 * 84000, alpha2,beta2, SDS-PAGE
86000
-
gel filtration or sucrose density gradient centrifugation in presence of 30% glycerol
94000
-
alpha2,beta2, 2 * 70000 + 2 * 94000, SDS-PAGE
94000 - 117000
-
gel filtration
additional information