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4.1.3.27: anthranilate synthase

This is an abbreviated version!
For detailed information about anthranilate synthase, go to the full flat file.

Word Map on EC 4.1.3.27

Reaction

chorismate
+
L-glutamine
=
anthranilate
 +
pyruvate
 +
L-glutamate

Synonyms

ANTA synthase, anthranilate synthase, anthranilate synthase a1, anthranilate synthase alpha 1, anthranilate synthase alpha 2, anthranilate synthase alpha2, anthranilate synthase component I, anthranilate synthase-phosphoribosyltransferase, anthranilate synthetase, AS-I, ASA, ASA1, ASA2, ASalpha, ASalphabeta, ASalphabeta-1, ASB, ASB1, CaASA1, CaASA2, chorismate lyase, Glutamine amido-transferase, Glutamine amidotransferase, HvASa2, OASA, OASA1, OASA1D, OASA2, PhnAB, PhnAB anthranilate synthase, synthase, anthranilate, synthetase, anthranilate, TrpDE, TrpE, TrpED

ECTree

     4 Lyases
         4.1 Carbon-carbon lyases
             4.1.3 Oxo-acid-lyases
                4.1.3.27 anthranilate synthase

Cloned

Cloned on EC 4.1.3.27 - anthranilate synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
alpha subunit (with a point mutation to confer feedback resistance to tryptophan) expressed in hairy root of Catharanthus roseus
-
cDNA(OASA1D) and the promoter of the maize ubiquitin gene is subcloned into pYT8C-Hm to generate pUASA1D, which also contains hpt as a selectable marker gene. For potato transformation, the vector p35SASA1D is constructed from pIG121-Hm, which contains the selectable marker genes hpt and nptII controlled by the 35S promoter of cauliflower mosaic virus, by replacing the beta-glucuronidase gene with the OASA1D cDNA
-
cloning of the genes phnA and phnB of the anthranilate synthase that participates in the synthesis of pyocyanin
-
expressed in a Catharanthus roseus hairy root line
-
expressed in Arabidopsis thaliana
-
expressed in Catharanthus roseus hairy roots
-
expressed in Escherichia coli
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli strain JM109
-
expressed in Glycine max
-
expressed in Pseudomonas putida strain KT2440
-
expressed in Vigna angularis
-
expression in Escherichia coli
expression in Nicotiana tabacum
expression in Saccharomyces cerevisiae
-
expression of gene trpE in Escherichia coli strain BL21(DE3)RIPL and of wild-type and mutant genes trpGD and trpG in Escherichia coli strain BL21(DE3)Rosetta, all as N- and C-terminally His6-tagged proteins
-
expression of OASA1D/N323 in Solanum tuberosum induces a 2fold to 20fold increase in the amount of Trp
-
gene phnAB, phylogenetic analysis, cloning in Escherichia coli strain Dh5alpha, enzyme expression in an enzyme-deficient Pseudomonas aeruginosa strain. Overexpression of anthranilate synthase genes trpE and phnAB results in pathway crosstalk, overview
gene trpE, phylogenetic analysis, cloning in Escherichia coli strain Dh5alpha, enzyme expression in an enzyme-deficient Pseudomonas aeruginosa strain. Overexpression of anthranilate synthase genes trpE and phnAB results in pathway crosstalk, overview
gene trpED
-
His-tagged veresion expressed in Escherichia coli C41(DE3)
-
into the BSMVm:gamma vector, the UbiNos_2-3 vector is used to express HvASa2. Into the hairpin vector pIPKTA30, hairpin construct co-bombarded with a Ubi:GUS expression vector into barley epidermal cells
into the pGEM-T Easy vector for expression in Escherichia coli cells
-
into the vector pET30a for expression in Escherichia coli BL21DE3 cells
into vector pAST-IV containing a 1671 bp long modified version of the ASA2 gene without a putative transit peptide and with an ASA2 3'-non-coding region (204 bp) as the termination sequence. Expression cassette containing Prrn-ASA2 integrated into the region between accD and ycf4 of the tobacco plastome
-
isolation of beta-subunit genes OASB1 and OASB2 and synthesis of each anthranilate synthase alpha-subunit (IASA1 and OASA2) and beta-subunit (OASB1 and OASB2) in wheat germ cell-free system
-
mutant anthanilate synthase Pro21Ser that is insensitive to feedback inhibition by Trp
-
partial complex anthranilate synthase expressed in Escherichia coli CB694
-
phylogenetic analysis, Agrobacterium-mediated co-overexpression of OsTDC, a putative tryptophan decarboxylase gene from rice, with OASA1D, the feedback-resistant anthranilate synthase alpha-subunit mutant in Oryza sativa calli, real-time quantitative reverse-transcription PCR expression analysis
-
quantitative real-time PCR enzyme expression analysis
transformation of Nicotiana tabacum with the ASA2 cDNA clone from Nicotiana tabacum driven by the CaMV 35S promoter and selected with kanamycin to obtain transformants that might be expressing ASA2
-
wild-type and mutant overexpressed in Escherichia coli cells (strain CB694) harboring the pSTC25 plasmid
-