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Literature summary for 4.1.3.27 extracted from
- Purification and characterization of anthranilate synthase component I (TrpE) from Mycobacterium tuberculosis H37Rv (2009), Protein Expr. Purif., 64, 8-15.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | TrpE is a promising target for the design of novel anti-tuberculosis drugs | Mycobacterium tuberculosis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the vector pET30a for expression in Escherichia coli BL21DE3 cells | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| L-tryptophan | - |
Mycobacterium tuberculosis |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Mycobacterium tuberculosis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62300 | - |
recombinant fusion protein, determined by SDS-PAGE | Mycobacterium tuberculosis |
| 62350 | - |
determined by mass spectrometry | Mycobacterium tuberculosis |
| 62370 | - |
calculated | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chorismate + ammonia | Mycobacterium tuberculosis | - |
anthranilate + pyruvate + H2O | - |
? | |
| chorismate + ammonia | Mycobacterium tuberculosis H37Rv | - |
anthranilate + pyruvate + H2O | - |
? | |
| chorismate + L-glutamine | Mycobacterium tuberculosis | - |
anthranilate + pyruvate + L-glutamate | - |
? | |
| chorismate + L-glutamine | Mycobacterium tuberculosis H37Rv | - |
anthranilate + pyruvate + L-glutamate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | P9WFX3 | - |
- |
| Mycobacterium tuberculosis H37Rv | P9WFX3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni-NTA His-binding resin, the 6xHis-tag is removed by digestion with enterokinase | Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.16 | - |
crude supernatant | Mycobacterium tuberculosis |
| 4.44 | - |
purification step Ni-NTA column | Mycobacterium tuberculosis |
| 5.2 | - |
after concentration step | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chorismate + ammonia | - |
Mycobacterium tuberculosis | anthranilate + pyruvate + H2O | - |
? | |
| chorismate + ammonia | - |
Mycobacterium tuberculosis H37Rv | anthranilate + pyruvate + H2O | - |
? | |
| chorismate + L-glutamine | - |
Mycobacterium tuberculosis | anthranilate + pyruvate + L-glutamate | - |
? | |
| chorismate + L-glutamine | - |
Mycobacterium tuberculosis H37Rv | anthranilate + pyruvate + L-glutamate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| anthranilate synthase component I | - |
Mycobacterium tuberculosis |
| TrpE | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
activity assay | Mycobacterium tuberculosis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 37 | incubation at temperatures higher than 37°C results in a significant reduction of activity | Mycobacterium tuberculosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
activity assay | Mycobacterium tuberculosis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 10 | pH 7 20% relative activity, pH 10 60% relative activity | Mycobacterium tuberculosis |
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