4.1.3.27: anthranilate synthase
This is an abbreviated version!
For detailed information about anthranilate synthase, go to the full flat file.
Word Map on EC 4.1.3.27
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4.1.3.27
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amidotransferase
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feedback-insensitive
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trpeg
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l-tryptophan
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p-aminobenzoate
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isochorismate
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catharanthus
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roseus
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glutamine-dependent
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5-methyltryptophan
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feedback-resistant
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chorismate-utilizing
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tryptamine
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paba
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buchnera
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prephenate
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indole-3-glycerol
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4-amino-4-deoxychorismate
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indoleglycerol
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5-phosphoribosylpyrophosphate
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aminodeoxychorismate
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biotechnology
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synthesis
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molecular biology
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medicine
- 4.1.3.27
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amidotransferase
-
feedback-insensitive
-
trpeg
- l-tryptophan
- p-aminobenzoate
- isochorismate
- catharanthus
- roseus
-
glutamine-dependent
- 5-methyltryptophan
-
feedback-resistant
-
chorismate-utilizing
- tryptamine
- paba
- buchnera
- prephenate
-
indole-3-glycerol
- 4-amino-4-deoxychorismate
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indoleglycerol
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5-phosphoribosylpyrophosphate
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aminodeoxychorismate
- biotechnology
- synthesis
- molecular biology
- medicine
Reaction
Synonyms
ANTA synthase, anthranilate synthase, anthranilate synthase a1, anthranilate synthase alpha 1, anthranilate synthase alpha 2, anthranilate synthase alpha2, anthranilate synthase component I, anthranilate synthase-phosphoribosyltransferase, anthranilate synthetase, AS-I, ASA, ASA1, ASA2, ASalpha, ASalphabeta, ASalphabeta-1, ASB, ASB1, CaASA1, CaASA2, chorismate lyase, Glutamine amido-transferase, Glutamine amidotransferase, HvASa2, OASA, OASA1, OASA1D, OASA2, PhnAB, PhnAB anthranilate synthase, synthase, anthranilate, synthetase, anthranilate, TrpDE, TrpE, TrpED
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Subunits
Subunits on EC 4.1.3.27 - anthranilate synthase
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dimer
heterodimer
heterotetramer
monomer
tetramer
additional information
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x * 60000-65000 + x + 60000-65000, Asalpha-subunit and Asbeta-subunit are of a similar MW
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1 * 14000 + 1 * 70000, gel filtration or sucrose density gradient centrifugation after separation of subunits
heterodimer
1 * 57000 + 1 * 23000, SDS-PAGE, 1 * 48500 + 1 * 21100, deduced from gene sequence
heterotetramer
association of 2 * enzyme + 2 * glutamine amidotransferase, crystallographic data
heterotetramer
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2 * 47743 + 2 * 21913, deduced from amino acid sequence
heterotetramer
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2 * 47743 + 2 * 21913, deduced from amino acid sequence
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heterotetramer
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association of 2 * enzyme + 2 * glutamine amidotransferase, crystallographic data
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heterotetramer
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crystallographic data
tetramer
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2 * 20000 + 2 * 50000, Gln-dependent enzyme, composed of 2 NH4+-dependent subunits and of 2 amidotransferase subunits, I2II2, gel filtration
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the large component from Bacillus subtilis (IB) complements well with the small component from Pseudomonas aeruginosa (IIP) to reconstitute a glutamine-reactive anthranilate synthase. Complementation was also observed with the large component from Pseudomonas aeruginosa (Iv) and the small subunit from Bacillus subtilis (IIB)
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one subunit is a trifunctional peptide which contains the catalytic sites for phosphoribosylanthranilate isomerase and indoleglycerol phosphate synthetase reactions, and associates with the second subunit to form Gln-dependent anthranilate synthetase
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recombinant alpha-subunit interacts with native beta-subunit to form an active enzyme
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the organism contains an NH4-dependent enzyme, i.e. component I, and a Gln-dependent enzyme that is composed of 2 NH4+-dependent subunits and of 2 amidotransferase subunits, i.e. I2II2
additional information
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the large component from Bacillus subtilis (IB) complements well with the small component from Pseudomonas aeruginosa (IIP) to reconstitute a glutamine-reactive anthranilate synthase. Complementation was also observed with the large component from Pseudomonas aeruginosa (Iv) and the small subunit from Bacillus subtilis (IIB)
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glutamine, 10 mM, enhances binding of AS I and II, as does Mg2+. ASI and ASII play a joint role in the binding of L-Gln, negligible binding to either component alone
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component I has a MW of 63000 Da, gel filtration
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multifunctional anthranilate synthetase-anthranilate 5-phosphoribosylpyrophosphate phosphoribosyltransferase
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multifunctional enzyme anthranilate synthase/N(5'-phosphoribosyl)anthranilate synthase, EC 4.1.3.27/EC 2.4.2.18, or ASI/ASII. ASI, is encoded by the gene TrpE and catalyzes the formation of anthranilate from chorismate and NH4+ as an independent subunit and from chorismate and Gln, when complexed with ASII. ASII consists of a glutamine amidotransferase domain, TrpG, which enables the complex to utilize Gln as an NH4+ source and an N-(5'-phosphoribosyl)anthranilate synthase domain, which catalyzes the production of N-(5'-phosphoribosyl)anthranilate from anthranilate and (5-phosphoribosyl)anthranilate
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partial complex anthranilate synthase consists of two TrpE:TrpG heterodimers
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subunit ASI catalyzes NH4+-dependent synthesis of anthranilate. Gln is not a substrate for ASI. ASII provides Gln-amide transfer function
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subunit ASI catalyzes NH4+-dependent synthesis of anthranilate. Gln is not a substrate for ASI. ASII provides Gln-amide transfer function
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additional information
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the 72000 Da subunit converts chorismate to anthranilate using NH4+. The 28000 Da subunit confers the enzyme the ability to use Gln instead of NH4+ as substrate
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the 72000 Da subunit converts chorismate to anthranilate using NH4+. The 28000 Da subunit confers the enzyme the ability to use Gln instead of NH4+ as substrate
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additional information
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synthase subunit TrpE and glutaminase subunit TrpG