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3.7.1.3: kynureninase

This is an abbreviated version!
For detailed information about kynureninase, go to the full flat file.

Word Map on EC 3.7.1.3

Reaction

L-kynurenine
+
H2O
=
anthranilate
 +
L-alanine

Synonyms

HsKynase, Kynase, KYNU, kynureninase, L-kynurenine hydrolase, PfKynase

ECTree

     3 Hydrolases
         3.7 Acting on carbon-carbon bonds
             3.7.1 In ketonic substances
                3.7.1.3 kynureninase

Purification

Purification on EC 3.7.1.3 - kynureninase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by chromatography on phenyl-Sepharose
-
by protamine sulfate precipitation, ammonium sulfate precipitation, DEAE-Sepharose 6B column, Sephadex G-100 column, Mono-Q HR 5/5 column, 50-80fold
-
by strong cationic column, strong anion exchange column and hydroxyapatite column
-
constitutive and inducible enzyme
-
His GraviTrap nickel affinity column chromatography
recombinant enzyme from Escherichia coli strain BL21(DE3)
recombinant enzyme from Escherichia coli strain DH5alpha
recombinant enzyme, ammonium sulfate precipitation, DEAE-Sepharose CL-6B affinity column, hydroxyapatite column, strong anion-exchange column at pH 8.6, strong cation-exchange column at pH 6.0 strong anion-exchange column at pH 6.0, 60fold
-
recombinant enzyme, by centrifugation, protamine sulfate precipitation, HiTrap Q column, omega-aminohexylagarose column, second HiTrap Q column and phenyl-Sepharose column
using a Ni-CAM resin column