Information on EC 3.7.1.3 - kynureninase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
3.7.1.3
-
RECOMMENDED NAME
GeneOntology No.
kynureninase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
L-kynurenine + H2O = anthranilate + L-alanine
show the reaction diagram
aldol and retro-aldol reactions; A pyridoxal-phosphate protein.; a pyridoxal-phosphate protein. Also acts on 3'-hydroxykynurenine and some other (3-acrylcarbonyl)-alanines, hydrolytic beta,gamma-cleavage of aryl-substituted gamma-keto-alpha-amino acids
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of C-C bond
-
-
-
-
hydrolysis of C-C bond
-
-
PATHWAY
KEGG Link
MetaCyc Link
Metabolic pathways
-
tryptophan degradation I (via anthranilate)
-
tryptophan degradation to 2-amino-3-carboxymuconate semialdehyde
-
tryptophan degradation XI (mammalian, via kynurenine)
-
Tryptophan metabolism
-
SYSTEMATIC NAME
IUBMB Comments
L-kynurenine hydrolase
A pyridoxal-phosphate protein. Also acts on 3'-hydroxy-L-kynurenine and some other (3-arylcarbonyl)-alanines.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Kynase
-
-
KYNU
B7XB98
-
kynureninase
-
-
kynureninase
Q16719
-
L-kynurenine hydrolase
-
-
-
-
L-kynurenine hydrolase
-
-
L-kynurenine hydrolase
-
-
L-kynurenine hydrolase
-
-
L-kynurenine hydrolase
-
-
L-kynurenine hydrolase
-
-
L-kynurenine hydrolase
-
-
CAS REGISTRY NUMBER
COMMENTARY
9024-78-6
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
strain ATCC 19213
-
-
Manually annotated by BRENDA team
strain red blood
UniProt
Manually annotated by BRENDA team
constitutive and inducible enzyme
-
-
Manually annotated by BRENDA team
male normolipidemic and diet-induced hyperlipidemic New Zealand rabbits; male Watanabe heritable hyperlipidemic rabbits
-
-
Manually annotated by BRENDA team
constitutive enzyme
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2S)-2-amino-4-oxo-4-phenylbutanoic acid + H2O
benzoate + L-alanine
show the reaction diagram
-
the rate-determining step is Cbeta-Cgamma bond cleavage
-
-
?
(2S,3S)-erythro-beta-methyl-L-kynurenine
?
show the reaction diagram
-
-
-
?
(4R)-5-bromodihydro-L-kynurenine + H2O
? + L-alanine
show the reaction diagram
-
slow substrate, no activity with (4S)-5-bromodihydro-L-kynurenine
-
-
?
(4R)-dihydro-L-kynurenine + H2O
2-aminobenzaldehyde + L-alanine
show the reaction diagram
-
-
-
?
(4R)-dihydro-L-kynurenine + H2O
2-aminobenzaldehyde + L-alanine
show the reaction diagram
-
-
-
-
?
2-amino-4-(2-chlorophenyl)-4-oxobutanoic acid + H2O
?
show the reaction diagram
-
-
-
-
?
2-amino-4-(2-methoxyphenyl)-4-oxobutanoic acid + H2O
?
show the reaction diagram
-
-
-
-
?
2-amino-4-(4-methoxyphenyl)-4-oxobutanoic acid + H2O
?
show the reaction diagram
-
-
-
-
?
2-fluorobenzoyl-DL-alanine + H2O
2-fluorobenzoate + beta-alanine
show the reaction diagram
-
-
-
-
?
3-bromo-L-kynurenine + H2O
3-bromoanthranilate + L-alanine
show the reaction diagram
-
-
-
-
?
3-hydroxy-DL-kynurenine + H2O
3-hydroxyanthranilate + DL-alanine
show the reaction diagram
Q16719
-
-
-
?
3-hydroxy-L-kynureinine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
Q16719
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
B7XB98, -
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
preferred substrate
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
biosynthesis of NAD
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
biosynthesis of NAD+
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
L-tryptophan metabolism, biosynthesis of nicotinamide nucleotides
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
the hydroxyl group at the 3-position thus appears to be an important recognition element
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
4-fluoro-L-kynurenine + H2O
4-fluoroanthranilate + L-alanine
show the reaction diagram
-
-
-
?
5-bromo-L-kynurenine + H2O
5-bromoanthranilate + L-alanine
show the reaction diagram
-
-
-
-
?
5-fluoro-L-kynurenine + H2O
5-fluoroanthranilate + L-alanine
show the reaction diagram
-
-
-
?
5-hydroxy-L-kynurenine + H2O
5-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
5-methyl-L-kynurenine + H2O
5-methylanthranilate + L-alanine
show the reaction diagram
-
-
-
?
beta-(4-chlorobenzoyl)-DL-alanine + H2O
4-chlorobenzoate + beta-alanine
show the reaction diagram
-
-
-
-
?
beta-(4-methylbenzoyl)-DL-alanine + H2O
4-methylbenzoate + beta-alanine
show the reaction diagram
-
-
-
-
?
beta-(4-trifluoromethylbenzoyl)-DL-alanine + H2O
4-trifluorobenzoate + beta-alanine
show the reaction diagram
-
-
-
-
?
beta-benzoyl-DL-alanine + H2O
benzoate + DL-alanine
show the reaction diagram
-
-
-
?
beta-benzoyl-L-alanine + H2O
benzoate + L-alanine
show the reaction diagram
-
-
-
-
?
beta-benzoyl-L-alanine + H2O
benzoate + L-alanine
show the reaction diagram
-
good substrate
-
-
?
beta-chloro-L-alanine + H2O
?
show the reaction diagram
-
-
-
?
gamma-(o-aminophenyl)-L-homoserine + H2O
L-alanine + o-aminobenzaldehyde
show the reaction diagram
-
-
-
?
kynurenine + H2O
excitotoxin quinolinate
show the reaction diagram
-
-
-
?
kynurenine + H2O
quinolinate
show the reaction diagram
-
-
-
?
L-alanine + pyridoxal 5'-phosphate
pyruvate + pyridoxamine 5'-phosphate
show the reaction diagram
-
-
-
?
L-alanine + pyridoxal 5'-phosphate
pyruvate + pyridoxamine 5'-phosphate
show the reaction diagram
-
-
-
?
L-alanine + pyridoxal 5'-phosphate
pyruvate + pyridoxamine 5'-phosphate
show the reaction diagram
-
-
-
?
L-kynurenine + benzaldehyde
(2S,4R)-2-amino-4-hydroxy-4-phenylbutanoic acid + ?
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
-
-
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
Q16719
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
B7XB98, -
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
L-tryptophan metabolism
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
L-tryptophan metabolism
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
L-tryptophan metabolism
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
L-tryptophan metabolism
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
P83788
L-tryptophan metabolism
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
L-tryptophan metabolism
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
the rate-determining step is to be deprotonation of the pyruvate-ketimine intermediate
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
the enzyme is involved in the catabolic pathway of L-tryptophan degradation, overview
-
-
?
L-ornithine + H2O
?
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
N-formylanthranilate + L-alanine
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
N-formylanthranilate + L-alanine
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
N-formylanthranilate + L-alanine
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
N-formylanthranilate + L-alanine
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
N-formylanthranilate + L-alanine
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
N-formylanthranilate + L-alanine
show the reaction diagram
-
-
-
?
N-formyl-L-kynurenine + H2O
N-formylanthranilate + L-alanine
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
Q16719
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
biosynthesis of NAD
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
biosynthesis of NAD+
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-alanine
show the reaction diagram
-
L-tryptophan metabolism, biosynthesis of nicotinamide nucleotides
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
3-hydroxy-L-kynurenine + H2O
3-hydroxyanthranilate + L-Ala
show the reaction diagram
-
-
-
?
5-hydroxy-L-kynurenine + H2O
5-hydroxyanthranilate + L-alanine
show the reaction diagram
-
-
-
?
5-methyl-L-kynurenine + H2O
5-methylanthranilate + L-alanine
show the reaction diagram
-
-
-
?
kynurenine + H2O
quinolinate
show the reaction diagram
-
-
-
?
L-alanine + pyridoxal 5'-phosphate
pyruvate + pyridoxamine 5'-phosphate
show the reaction diagram
-
-
-
?
L-alanine + pyridoxal 5'-phosphate
pyruvate + pyridoxamine 5'-phosphate
show the reaction diagram
-
-
-
?
L-alanine + pyridoxal 5'-phosphate
pyruvate + pyridoxamine 5'-phosphate
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
L-tryptophan metabolism
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
L-tryptophan metabolism
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
L-tryptophan metabolism
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
L-tryptophan metabolism
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
P83788
L-tryptophan metabolism
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
L-tryptophan metabolism
-
-
?
L-kynurenine + H2O
anthranilate + L-alanine
show the reaction diagram
-
the enzyme is involved in the catabolic pathway of L-tryptophan degradation, overview
-
-
?
COFACTOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
-
pyridoxal 5'-phosphate
-
-
pyridoxal 5'-phosphate
-
-
pyridoxal 5'-phosphate
-
dependent on
pyridoxal 5'-phosphate
-
dependent on
vitamin B6
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
(2S)-2-amino-4-oxo-4-phenylbutanoic acid
-
competitive inhibitor of the reaction of L-kynurenine with the enzyme
(4R)-5-bromodihydro-L-kynurenine
-
-
(4S)-5-bromodihydro-L-kynurenine
-
-
(4S)-dihydro-L-kynurenine
-
competitive inhibitor
(4S)-dihydro-L-kynurenine
-
-
(S)-(2-amino-4-bromophenyl)-L-cysteine S,S-dioxide
-
competitive inhibition
(S)-(2-amino-5-bromophenyl)-L-cysteine S,S-dioxide
-
competitive inhibition
(S)-(2-aminophenyl)-L-cysteine S,S-dioxide
-
-
2-amino-(4-oxo-4-naphthyl)-butyric acid
-
competitive inhibition, reversible inhibition by dilution
2-amino-3-fluoro-S-phenyl cysteine S,S-dioxide
-
-
2-amino-3-methoxy-S-phenyl cysteine S,S-dioxide
-
-
2-amino-4-[3'-hydroxyphenyl]-4-hydroxybutanoic acid
-
most potent inhibitor
2-amino-5-methyl-S-phenyl cysteine S,S-dioxide
-
-
2-amino-S-phenyl cysteine S,S-dioxide
-
-
3,5-dihydroxydeaminokynurenine
-
-
3,7-dihydroxy-deamino-DL-kynurenine
-
mixed inhibition
3-guanidino-L-alanine
-
0.001 mM in 0.2 mM Tris-HCl buffer
3-Hydroxy-DL-kynurenine
-
-
3-Hydroxyanthranilate
-
0.001 mM in 0.06 mM Tris-HCl buffer
3-hydroxydeaminokynurenine
-
competitive and non competitive inhibition
3-hydroxyhippuric acid
-
competitive inhibitor of kynureninase
3-methoxydeaminokynurenine
-
competitive and non competitive inhibition
amino-(1-oxo-1,2,3,4-tetrahydro-2-naphthalenyl)-acetic acid
-
competitive inhibition, reversible inhibition by dilution
amino-(1-oxo-2,3-dihydro-1H-inden-2-yl)-acetic acid
-
competitive inhibition, reversible inhibition by dilution
amino-(4-oxo-3,4-dihydro-2H-chromen-3-yl)-acetic acid
-
competitive inhibition, reversible inhibition by dilution
anthranilic acid
-
1 mM
anthranilic acid
-
-
anthranilic acid
-
-
beta-Alanine
-
competitive inhibition, 10 mM
beta-benzoyl-L-alanine
-
competitive inhibition, at 25C, pH 7.8
beta-chloro-L-alanine
-
irreversible inhibition
cadaverine
-
competitive inhibition, 10 mM
D-alanine
-
competitive inhibition, 10 mM
D-cycloserine
-
1 mM
D-cycloserine
-
-
D-kynurenine
-
-
D-kynurenine
-
mixed inhibition
D-penicillamine
-
1 mM
D-penicillamine
-
-
D-serine
-
competitive inhibition, 10 mM
deaminokynurenine
-
competitive and non competitive inhibition
delta-N-acetyl-L-ornithine
-
0.001 mM in 0.06 mM Tris-HCl buffer
delta-N-acetyl-L-ornithine
-
-
dihydro-L-kynurenine
-
-
DL-5-chloro-kynurenine
-
substantially inhibits
DL-allothreonine
-
competitive inhibition, 10 mM
DL-alpha-aminobutyric acid
-
competitive inhibition, 10 mM
DL-dihydroxyphenylalanine
-
competitive inhibition, 5 mM
DL-dihydroxyphenylalanine
-
-
DL-homoserine
-
competitive inhibition, 10 mM
DL-methionine
-
competitive inhibition, 40 mM
DL-Ornithine
-
competitive inhibition, 10 mM
iodoacetate
-
0.5 mM
Kynurenate
-
0.001 mM in 0.06 mM Tris-HCl buffer
L-5-chloro-kynurenine
-
substantially inhibits
L-alanine
-
competitive inhibition, 40 mM
L-arginine
-
competitive inhibition, 10 mM
L-asparagine
-
competitive inhibition, 10 mM
L-aspartic acid
-
competitive inhibition, 10 mM
L-cysteine
-
competitive inhibition, 10 mM
L-Glutamic acid
-
competitive inhibition, 10 mM
L-glutamine
-
competitive inhibition, 10 mM
L-histidine
-
competitive inhibition, 10 mM
L-kynurenine
-
-
L-kynurenine
-
competitive inhibition, at low substrate concentrations; non-competitive inhibition, at higher levels of substrate
L-lysine
-
competitive inhibition, 10 mM
L-ornithine
-
0.001 mM in 0.2 mM Tris-HCl buffer
L-Penicillamine
-
-
L-serine
-
competitive inhibition, 10 mM
L-threonine
-
competitive inhibition, 10 mM
L-tryptophan
-
competitive inhibition, 10 mM
lanthellamide
-
about 60% residual activity at 1 mM, about 80% residual activity at 0.5 mM, slight inhibition at 0.125 mM
-
N'-formyl-L-kynurenine
-
-
N-ethylmaleimide
-
1 mM
nicotinylalanine
-
-
nicotinylalanine
-
1 mM
o-aminoacetophenone
-
1 mM
o-aminoacetophenone
-
-
o-aminobenzaldehyde
-
0.1 mM
o-aminobenzaldehyde
-
-
o-bromobenzoylalanine
-
-
o-ethoxybenzoylalanine
-
-
o-fluorobenzoylalanine
-
-
o-methoxybenzoylalanine
-
-
o-methylbenzoylalanine
-
-
o-nitrobenzaldehyde
-
1 mM
o-nitrobenzaldehyde
-
-
o-trifluoromethylbenzoylalanine
-
-
p-chloromercuribenzoate
-
0.5 mM
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
p-chloromercuribenzoate
-
-
p-hydroxyphenylpyruvate
-
5 mM
phenylhydrazine-HCl
-
1 mM
phenylhydrazine-HCl
-
-
phenylhydrazine-HCl
-
-
phenylhydrazine-HCl
-
-
phenylpyruvate
-
1 mM
putrescine
-
competitive inhibition, 10 mM
-
putrescine
-
-
-
pyridoxal 5'-phosphate
-
-
quinaldinate
-
-
quinaldinate
-
0.001 mM in 0.06 mM Tris-HCl buffer
S-(2-aminophenyl)-L-cysteine S,S-dioxide
-
transition state analogue
S-(2-aminophenyl)-L-cysteine S,S-dioxide
-
-
S-(beta-aminoethyl)-L-cysteine
-
0.001 mM in 0.06 mM Tris-HCl buffer
Semicarbazide
-
1 mM
Semicarbazide
-
-
Semicarbazide
-
-
Sodium borohydride
-
-
Sodium borohydride
-
-
tryptamine
-
competitive inhibition, 10 mM
m-nitrobenzoylalanine
-
-
additional information
-
not inhibited by DL-4-(ethylsulfonyl)benzoylalanine and its active enatiomer (S)-4-(ethylsulfonyl)benzoylalanine
-
additional information
-
catabolite repression of the pathway by glucose and glutamate, arginine does not cause repression
-
additional information
-
lanthellamide does not inhibit the enzyme at concentrations up to 0.0625 mM
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
-
pyridoxal 5'-phosphate
-
in the presence of pyruvate
pyridoxal 5'-phosphate
-
-
pyridoxamine phosphate
-
in the presence of pyruvate
additional information
-
kynurenine induces both tryptophan oxygenase and kynureninase of the catabolic pathway of L-tryptophan degradation
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
2
-
3-bromo-L-kynurenine
-
-
0.0283
-
3-Hydroxy-DL-kynurenine
-
-
0.003
-
3-Hydroxy-L-kynurenine
-
-
0.005
-
3-Hydroxy-L-kynurenine
-
-
0.0283
-
3-Hydroxy-L-kynurenine
-
wild type enzyme, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37C
0.0442
-
3-Hydroxy-L-kynurenine
-
at 37C, pH 7.5
0.2
-
3-Hydroxy-L-kynurenine
-
-
0.2
-
3-Hydroxy-L-kynurenine
-
Km above 0.2 mM, mutant enzyme N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37C
0.51
-
3-Hydroxy-L-kynurenine
-
Km above 0.51 mM, mutant enzyme S332G/N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37C
3
-
3-Hydroxy-L-kynurenine
-
pH 7.9, at 37C
0.003
-
3-hydroxykynurenine
-
-
0.0039
-
3-hydroxykynurenine
-
-
0.0046
-
3-hydroxykynurenine
-
-
0.0067
-
3-hydroxykynurenine
-
-
0.067
-
4-fluoro-L-kynurenine
-
-
0.011
-
5-bromo-L-kynurenine
-
-
0.048
-
5-fluoro-L-kynurenine
-
-
0.008
-
beta-benzoyl-L-alanine
-
at 25C, pH 7.8
0.16
-
beta-benzoyl-L-kynurenine
-
-
0.067
-
gamma-(o-aminophenyl)-L-homoserine
-
-
0.006
-
kynurenine
-
-
0.01
-
kynurenine
-
-
0.025
-
kynurenine
-
-
0.08
-
kynurenine
-
-
0.013
-
L-3-Hydroxykynurenine
-
-
0.013
-
L-3-Hydroxykynurenine
-
-
0.018
-
L-3-Hydroxykynurenine
-
-
0.035
-
L-3-Hydroxykynurenine
-
-
0.037
-
L-3-Hydroxykynurenine
-
constitutive enzyme
0.05
-
L-3-Hydroxykynurenine
-
-
0.077
-
L-3-Hydroxykynurenine
-
-
0.2
-
L-3-Hydroxykynurenine
-
-
0.2
-
L-3-Hydroxykynurenine
-
-
0.22
-
L-3-Hydroxykynurenine
-
-
0.54
-
L-3-Hydroxykynurenine
-
constitutive enzyme
11
-
L-alanine
-
-
0.0067
-
L-kynurenine
-
constitutive enzyme
0.018
-
L-kynurenine
-
-
0.025
-
L-kynurenine
-
-
0.03
-
L-kynurenine
-
at 25C, pH 7.8
0.034
-
L-kynurenine
-
constitutive enzyme
0.035
-
L-kynurenine
-
-
0.035
-
L-kynurenine
-
-
0.035
-
L-kynurenine
-
-
0.039
0.0714
L-kynurenine
-
-
0.0442
-
L-kynurenine
-
at 37C, pH 7.5
0.0442
-
L-kynurenine
-
-
0.2
-
L-kynurenine
-
-
0.24
-
L-kynurenine
-
-
0.24
-
L-kynurenine
-
-
0.25
-
L-kynurenine
-
-
0.327
-
L-kynurenine
-
mutant enzyme H102W/N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37C
0.4
-
L-kynurenine
-
-
0.495
-
L-kynurenine
-
wild type enzyme, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37C
0.499
-
L-kynurenine
-
mutant enzyme N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37C
0.5
-
L-kynurenine
-
-
0.51
-
L-kynurenine
-
Km above 0.51 mM, mutant enzyme H102W/S332G/N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37C
0.54
-
L-kynurenine
-
mutant enzyme S332G/N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37C
0.77
-
L-kynurenine
-
-
1
-
L-kynurenine
-
-
40
-
L-ornithine
-
-
2.2
-
N'-formyl-L-kynurenine
-
-
2.2
-
N'-formyl-L-kynurenine
-
-
0.0018
-
pyridoxal 5'-phosphate
-
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.7
-
(2S)-2-amino-4-oxo-4-phenylbutanoic acid
-
at pH 7.8
0.08
-
2-amino-4-(2-chlorophenyl)-4-oxobutanoic acid
-
at 37C in 0.04 M potassium phosphate, pH 7.8
-
0.05
-
2-amino-4-(2-methoxyphenyl)-4-oxobutanoic acid
-
at 37C in 0.04 M potassium phosphate, pH 7.8
-
0.38
-
2-amino-4-(4-methoxyphenyl)-4-oxobutanoic acid
-
at 37C in 0.04 M potassium phosphate, pH 7.8
-
0.06
-
2-fluorobenzoyl-DL-alanine
-
at 37C in 0.04 M potassium phosphate, pH 7.8
-
3.5
-
3-Hydroxy-DL-kynurenine
-
-
0.0039
-
3-Hydroxy-L-kynurenine
-
kcat less than 0.0039 1/s, mutant enzyme N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37C
0.0109
-
3-Hydroxy-L-kynurenine
-
kcat less than 0.0109 1/s, mutant enzyme S332G/N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37C
3.5
-
3-Hydroxy-L-kynurenine
-
wild type enzyme, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37C
1.83
-
beta-(4-chlorobenzoyl)-DL-alanine
-
at 37C in 0.04 M potassium phosphate, pH 7.8
-
0.91
-
beta-(4-methylbenzoyl)-DL-alanine
-
at 37C in 0.04 M potassium phosphate, pH 7.8
-
0.001
-
beta-(4-trifluoromethylbenzoyl)-DL-alanine
-
at 37C in 0.04 M potassium phosphate, pH 7.8
-
0.7
-
beta-benzoyl-L-alanine
-
-
0.007
-
L-kynurenine
-
mutant enzyme H102W/N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37C
0.017
-
L-kynurenine
-
mutant enzyme S332G/N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37C
0.018
-
L-kynurenine
-
Km above 0.51 mM, mutant enzyme H102W/S332G/N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37C
0.026
-
L-kynurenine
-
mutant enzyme N333T, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37C
0.23
-
L-kynurenine
-
wild type enzyme, in 30 mM potassium phosphate, pH 8.0, and 0.04 mM pyridoxal 5'-phosphate, at 37C
16
-
L-kynurenine
-
; at pH 7.8
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
12
-
2-amino-4-(2-chlorophenyl)-4-oxobutanoic acid
-
at 37C in 0.04 M potassium phosphate, pH 7.8
0
1.4
-
2-amino-4-(2-methoxyphenyl)-4-oxobutanoic acid
-
at 37C in 0.04 M potassium phosphate, pH 7.8
0
1.6
-
2-amino-4-(4-methoxyphenyl)-4-oxobutanoic acid
-
at 37C in 0.04 M potassium phosphate, pH 7.8
0
1.7
-
2-fluorobenzoyl-DL-alanine
-
at 37C in 0.04 M potassium phosphate, pH 7.8
0
18
-
beta-(4-chlorobenzoyl)-DL-alanine
-
at 37C in 0.04 M potassium phosphate, pH 7.8
0
28
-
beta-(4-methylbenzoyl)-DL-alanine
-
at 37C in 0.04 M potassium phosphate, pH 7.8
0
0.019
-
beta-(4-trifluoromethylbenzoyl)-DL-alanine
-
at 37C in 0.04 M potassium phosphate, pH 7.8
0
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.01
-
(2S)-2-amino-4-oxo-4-phenylbutanoic acid
-
reaction of L-kynurenine with the enzyme
5.5e-05
-
(4R)-5-bromodihydro-L-kynurenine
-
-
0.00017
-
(4S)-5-bromodihydro-L-kynurenine
-
-
0.0003
-
(S)-(2-amino-4-bromophenyl)-L-cysteine S,S-dioxide
-
-
0.0004
-
(S)-(2-amino-5-bromophenyl)-L-cysteine S,S-dioxide
-
-
2.7e-05
-
(S)-(2-aminophenyl)-L-cysteine S,S-dioxide
-
-
0.005
-
2-amino-(4-oxo-4-naphthyl)-butyric acid
-
at 37C, pH 7.5
0.022
-
2-amino-(4-oxo-4-naphthyl)-butyric acid
-
at 37C, pH 7.5
0.175
-
2-amino-4-(3-methoxyphenyl)-4-oxobutanoic acid
-
-
0.00025
-
3,5-dihydroxydeaminokynurenine
-
-
5e-06
-
3-hydroxydeaminokynurenine
-
-
4e-05
-
3-hydroxydeaminokynurenine
-
-
0.01
-
3-methoxydeaminokynurenine
-
-
0.015
-
3-methoxydeaminokynurenine
-
-
0.17
-
amino-(1-oxo-1,2,3,4-tetrahydro-2-naphthalenyl)-acetic acid
-
at 37C, pH 7.5
0.227
-
amino-(1-oxo-1,2,3,4-tetrahydro-2-naphthalenyl)-acetic acid
-
at 37C, pH 7.5
0.0349
-
amino-(1-oxo-2,3-dihydro-1H-inden-2-yl)-acetic acid
-
at 37C, pH 7.5
0.045
-
amino-(1-oxo-2,3-dihydro-1H-inden-2-yl)-acetic acid
-
at 37C, pH 7.5
0.077
-
amino-(4-oxo-3,4-dihydro-2H-chromen-3-yl)-acetic acid
-
at 37C, pH 7.5
0.162
-
amino-(4-oxo-3,4-dihydro-2H-chromen-3-yl)-acetic acid
-
at 37C, pH 7.5
0.01
-
beta-benzoyl-L-alanine
-
at 25C, pH 7.8
0.012
-
D-kynurenine
-
-
0.012
-
D-kynurenine
-
pH 7.9, at 37C
0.012
-
deaminokynurenine
-
-
0.02
-
L-kynurenine
-
-
0.02
-
L-kynurenine
-
pH 7.9, at 37C, at low substrate concentrations
0.055
-
L-kynurenine
-
pH 7.9, at 37C, at higher levels of substrate
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
1e-05
-
-
kidney, approximately
1.8e-05
-
-
liver
1.9e-05
-
-
liver of diet-induced hyperlipidemic animals
2e-05
-
-
kidney; liver of normolipidemic animals
2.1e-05
-
-
kidney of normolipidemic animals
2.2e-05
-
-
kidney of diet-induced hyperlipidemic animals
3e-05
-
-
liver, kidney, approximate values
4e-05
-
-
liver, approximately
6e-05
-
-
liver, kidney, approximate values
9e-05
-
-
liver, kidney, approximate values
0.0002
-
-
liver
0.0007
-
-
liver
0.008
0.015
-
pH 7.5, 37C
0.0178
-
-
-
0.026
-
-
liver
0.164
-
-
pH 7.9, 37C
0.3
-
-
-
0.661
-
-
-
1.2
2.2
-
pH 7.5, 37C
1.75
-
-
3-hydroxy-DL-kynurenine
4.4
-
-
cells grown on L-asparagine
8.8
-
-
cells grown on L-arginine
10.3
-
-
cells grown on L-alanine
31.25
-
-
cells grown on L-tryptophan
68.3
-
-
-
additional information
-
-
19 U/mg protein
additional information
-
-
poor activity in cells grown on arginine and on glucose, also in presence of L-tryptophan
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7
-
-
assay at
7
-
-
activity assay
8.3
-
-
-
8.5
-
-
constitutive enzyme
8.5
-
-
constitutive enzyme
8.5
-
-
-
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
-
-
assay at
37
-
-
activity assay
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
cells grown in a medium containing L-tryptophan as the sole carbon, nitrogen, and energy source
Manually annotated by BRENDA team
-
enzyme activity does not change significantly as compared to controls; enzyme activity does not change significantly in normolipidemic and diet-induced hyperlipidemic animals as compared to controls
Manually annotated by BRENDA team
-
enzyme activity is very low in newborn rats, but increases in rats of 2-3 months to 18 months of age, without an evident age-related pattern
Manually annotated by BRENDA team
-
the catalytic activity of kynureninase is very low in most tissue with exception of liver and kidney
Manually annotated by BRENDA team
-
enzyme activity significantly decreased only per g of fresh tissue as compared to controls; enzyme activity significantly decreased only per g of fresh tissue in the group of diet-induced hyperlipidemic animals as compared to controls
Manually annotated by BRENDA team
-
enzyme activity declines with age, similar in newborns and 2-3 month-old rats, activity decreases markedly by ca. 60% and 70% at 12 and 18 months, respectively
Manually annotated by BRENDA team
-
the catalytic activity of kynureninase is very low in most tissue with exception of liver and kidney
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
45790
-
-
ESI-MS
45820
-
-
MALDI-TOF
48600
-
B7XB98, -
calculated from amino acid sequence
49000
-
B7XB98, -
approximately 49000 Da, SDS-PAGE
52400
-
-
recombinant enzyme, SDS-PAGE
52400
-
-
recombinant enzyme, MALDI-TOF MS
91000
-
-
gel filtration
92000
-
-
gel filtration
95000
-
-
sucrose density gradient centrifugation
95000
-
-
native enzyme, disc gel electrophoresis without SDS and mercaptoethanol
100000
-
-
sedimentation equilibrium method
100000
-
-
gel filtration
105000
-
-
gel filtration
105000
-
-
-
105000
-
-
gel filtration
110000
-
-
constitutive and inducible enzyme, gel filtration
110000
-
-
-
130000
-
-
-
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
dimer
-
2 * 50000
dimer
-
2 * 46000, SDS-PAGE
dimer
-
2 * 55000, SDS-PAGE
homodimer
-
native enzyme, 2 * 52500, disc gel electrophoresis
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
no modification
-
-
no modification
-
-
no modification
-
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
crystals of recombinant kynureninase that diffracted to 2.0 A are obtained, and the atomic structure of the PLP-bound holoenzyme is determined by molecular replacement using the Pseudomonas fluorescens structure as the phasing model
-
the wild type enzyme is cocrystallized with 3-hydroxyhippuric acid, using the modified microbatch under oil technique at 25?C, with 0.05 M MgCl2, 0.1 M Tris (pH 8.0), and 25% (w/v) PEG 3000
-
by hanging-drop vapor diffusion method at room temperature, to 1.85 A resolution, Lys-227 is the pyridoxal-5'-phosphate binding residue near the pyridoxal-5'-phosphate nitrogen, but Asp-132 is also strictly conserved and at a similar distance from the pyridinium nitrogen, Tyr-226 donates a hydrogen bond to the phosphate of pyridoxal-5'-phosphate, Trp-256 donates a hydrogen bond to the phosphate through the indole N1-hydrogen; performed using the hanging-drop vapor diffusion technique
P83788
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-80C, pyridoxal 5'-phosphate, more than 12 months, no loss of activity
-
-80C, without pyridoxal 5'-phosphate, more than 12 months, no loss of activity
-
4C, pyridoxal 5'-phosphate, up to 2 weeks, stable
-
-78C, 0.1 mM potassium phosphate buffer, 0.2 mM pyridoxal 5'-phosphate, pH 8.0, 1 year, stable
P83788
-78C, 5 mM potassium phosphate buffer, 0.2 mM pyridoxal 5'-phosphate, pH 8.0, 1 month, loses 4% of activity
P83788
4C, 5 mM potassium phosphate buffer, 0.2 mM pyridoxal 5'-phosphate, pH 8.0, 1 week, loses 40% of activity
P83788
78C, phosphate buffer, 1 year
P83788
4C, phosphate buffer, pH 7.2, 0.02 mM pyridoxal-5'-phosphate, 1 week
-
70% saturated solution of ammonium sulfate containing 1 mM EDTA, 1 mM dithiothreitol, and 0.05 mM pyridoxal 5'-phosphate, 2 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
His GraviTrap nickel affinity column chromatography
B7XB98, -
by strong cationic column, strong anion exchange column and hydroxyapatite column
-
recombinant enzyme, ammonium sulfate precipitation, DEAE-Sepharose CL-6B affinity column, hydroxyapatite column, strong anion-exchange column at pH 8.6, strong cation-exchange column at pH 6.0 strong anion-exchange column at pH 6.0, 60fold
-
using a Ni-CAM resin column
-
constitutive and inducible enzyme
-
; by chromatography on phenyl-Sepharose
-
; recombinant enzyme, by centrifugation, protamine sulfate precipitation, HiTrap Q column, omega-aminohexylagarose column, second HiTrap Q column and phenyl-Sepharose column
P83788
by protamine sulfate precipitation, ammonium sulfate precipitation, DEAE-Sepharose 6B column, Sephadex G-100 column, Mono-Q HR 5/5 column, 50-80fold
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
expressed in Escherichia coli
B7XB98, -
expression in Sf9 insect cells
-
expression in Spodoptera frugiperda cells
-
into a pET100 plasmid for expression in Escherichia coli BL21DE3 cells
-
overexpression is achieved with the Bac-to-Bac baculovirus expression system
-
expressed in Escherichia coli DH-5alpha cells
-
expression in Escherichia coli; into vector pTZKYN and transformed in Escherichia coli XL-1 Blue cells
-
expression in Escherichia coli; subcloned into pSapKO-WT and transformed into Escherichia coli B834(DE3)
P83788
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
H102W/N333T
-
the mutant shows complete reversal of substrate specificity between 3-hydroxy-L-kynurenine and L-kynurenine
H102W/S332G/N333T
-
the mutant shows complete reversal of substrate specificity between 3-hydroxy-L-kynurenine and L-kynurenine
N333T
-
the mutant reveals a 9fold decrease in kcat for L-kynurenine, but no change in Km, the mutant has weaker 3-hydroxy-L-kyrenine binding (6fold higher Km) and kcat at least 1100times slower than wild type Kynase
S332G/N333T the
-
mutant shows a very slight preference for L-kynurenine over 3-hydroxy-L-kynurenine
T198A
-
screening of cDNA from KYNU revealed homozygosity for a c.593A>G substitution in exon 7 in the proband, leading to a threonine-to-alanine shift, T198A, in kynureninase
D132A
P83788
has lower activity and binds pyridoxal 5'-phosphate weakly; has lower activity and weaklier pyridoxal-5'-phosphate binding than that of wild-type enzyme
D132E
P83788
has good activity and pyridoxal 5'-phosphate binding stronger than that of the wild-type enzyme; has good activity and stronger pyridoxal-5'-phosphate binding than that of wild-type enzyme
D201A
P83788
has very low activity and binds pyridoxal-5'-phosphate weakly, and expresses poorly, giving large amounts of insoluble inclusion bodies and very low levels of soluble protein; has very low activity, less than 0.01% of that of the wild-type enzyme, binds pyridoxal 5'-phosphate weakly
T102I
B7XB98, -
the mutation leads to a marked decrease in kynureninase activity and causes the red body coloration in the Bombyx mori red blood strain
additional information
-
cosmid pRA3 corrects trpA, trpB and trpC mutants in Escherichia coli
D201E
P83788
has good activity and pyridoxal 5'-phosphate binding stronger than that of the wild-type enzyme; has good activity and stronger pyridoxal-5'-phosphate binding than that of wild-type enzyme
additional information
-
cosmid pRA3 corrects trpA mutants of Rhizobium leguminosarum
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
medicine
-
tryptophan metabolism
medicine
-
tryptophan metabolism
medicine
-
tryptophan metabolism
medicine
-
xanthurenic aciduria is linked to a mutation in the gene encoding kynureninase
synthesis
-
of inhibitors of kynureninase could prove to be useful in the development of the successful treatment regimen for neurological disorders such as septicemia, AIDS related dementia, Lyme disease, Huntington's and Alzheimer's disease
medicine
-
tryptophan metabolism
medicine
-
tryptophan metabolism
medicine
-
tryptophan metabolism
medicine
-
NAD+ biosynthesis; tryptophan metabolism
medicine
-
NAD+ biosynthesis; tryptophan metabolism
medicine
-
tryptophan metabolism
medicine
-
tryptophan metabolism
medicine
-
NAD+ biosynthesis; tryptophan metabolism
synthesis
-
of inhibitors of kynureninase could prove to be useful in the development of the successful treatment regimen for neurological disorders such as septicemia, AIDS related dementia, Lyme disease, Huntington's and Alzheimer's disease
medicine
-
tryptophan metabolism
medicine
-
NAD+ biosynthesis
medicine
-
NAD+ biosynthesis; tryptophan metabolism
medicine
-
tryptophan metabolism
medicine
-
NAD+ biosynthesis
medicine
-
progressive decline in the enzyme activity involved in tryptophan metabolism along the kynurenine pathway in rat tissue with age
medicine
-
tryptophan metabolism
medicine
-
tryptophan metabolism
medicine
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NAD+ biosynthesis; tryptophan metabolism
medicine
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tryptophan metabolism
medicine
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tryptophan metabolism
medicine
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tryptophan metabolism
additional information
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cosmid pRA3 contains a trp operon that resembles that of the pathogen Chlamydophila caviae, containing the unusual kynU gene, which specifies kynureinase
synthesis
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of inhibitors of kynureninase could prove to be useful in the development of the successful treatment regimen for neurological disorders such as septicemia, AIDS related dementia, Lyme disease, Huntington's and Alzheimer's disease
additional information
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cosmid pRA3 contains a trp operon that resembles that of the pathogen Chlamydophila caviae, containing the unusual kynU gene, which specifies kynureinase