Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH-5alpha cells | Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-kynurenine + H2O | Pseudomonas fluorescens | - |
anthranilate + L-alanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-4-(2-chlorophenyl)-4-oxobutanoic acid + H2O | - |
Pseudomonas fluorescens | ? | - |
? | |
2-amino-4-(2-methoxyphenyl)-4-oxobutanoic acid + H2O | - |
Pseudomonas fluorescens | ? | - |
? | |
2-amino-4-(4-methoxyphenyl)-4-oxobutanoic acid + H2O | - |
Pseudomonas fluorescens | ? | - |
? | |
2-fluorobenzoyl-DL-alanine + H2O | - |
Pseudomonas fluorescens | 2-fluorobenzoate + beta-alanine | - |
? | |
beta-(4-chlorobenzoyl)-DL-alanine + H2O | - |
Pseudomonas fluorescens | 4-chlorobenzoate + beta-alanine | - |
? | |
beta-(4-methylbenzoyl)-DL-alanine + H2O | - |
Pseudomonas fluorescens | 4-methylbenzoate + beta-alanine | - |
? | |
beta-(4-trifluoromethylbenzoyl)-DL-alanine + H2O | - |
Pseudomonas fluorescens | 4-trifluoromethylbenzoate + beta-alanine | - |
? | |
beta-benzoyl-L-alanine + H2O | good substrate | Pseudomonas fluorescens | benzoate + L-alanine | - |
? | |
L-kynurenine + H2O | - |
Pseudomonas fluorescens | anthranilate + L-alanine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Kynase | - |
Pseudomonas fluorescens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
beta-(4-trifluoromethylbenzoyl)-DL-alanine | at 37°C in 0.04 M potassium phosphate, pH 7.8 | Pseudomonas fluorescens | |
0.05 | - |
2-amino-4-(2-methoxyphenyl)-4-oxobutanoic acid | at 37°C in 0.04 M potassium phosphate, pH 7.8 | Pseudomonas fluorescens | |
0.06 | - |
2-fluorobenzoyl-DL-alanine | at 37°C in 0.04 M potassium phosphate, pH 7.8 | Pseudomonas fluorescens | |
0.08 | - |
2-amino-4-(2-chlorophenyl)-4-oxobutanoic acid | at 37°C in 0.04 M potassium phosphate, pH 7.8 | Pseudomonas fluorescens | |
0.38 | - |
2-amino-4-(4-methoxyphenyl)-4-oxobutanoic acid | at 37°C in 0.04 M potassium phosphate, pH 7.8 | Pseudomonas fluorescens | |
0.91 | - |
beta-(4-methylbenzoyl)-DL-alanine | at 37°C in 0.04 M potassium phosphate, pH 7.8 | Pseudomonas fluorescens | |
1.83 | - |
beta-(4-chlorobenzoyl)-DL-alanine | at 37°C in 0.04 M potassium phosphate, pH 7.8 | Pseudomonas fluorescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Pseudomonas fluorescens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
beta-(4-trifluoromethylbenzoyl)-DL-alanine | at 37°C in 0.04 M potassium phosphate, pH 7.8 | Pseudomonas fluorescens | |
1.4 | - |
2-amino-4-(2-methoxyphenyl)-4-oxobutanoic acid | at 37°C in 0.04 M potassium phosphate, pH 7.8 | Pseudomonas fluorescens | |
1.6 | - |
2-amino-4-(4-methoxyphenyl)-4-oxobutanoic acid | at 37°C in 0.04 M potassium phosphate, pH 7.8 | Pseudomonas fluorescens | |
1.7 | - |
2-fluorobenzoyl-DL-alanine | at 37°C in 0.04 M potassium phosphate, pH 7.8 | Pseudomonas fluorescens | |
12 | - |
2-amino-4-(2-chlorophenyl)-4-oxobutanoic acid | at 37°C in 0.04 M potassium phosphate, pH 7.8 | Pseudomonas fluorescens | |
18 | - |
beta-(4-chlorobenzoyl)-DL-alanine | at 37°C in 0.04 M potassium phosphate, pH 7.8 | Pseudomonas fluorescens | |
28 | - |
beta-(4-methylbenzoyl)-DL-alanine | at 37°C in 0.04 M potassium phosphate, pH 7.8 | Pseudomonas fluorescens |