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Literature summary for 3.7.1.3 extracted from
- Crystal structure of Homo sapiens kynureninase (2007), Biochemistry, 46, 2735-2744.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into a pET100 plasmid for expression in Escherichia coli BL21DE3 cells | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystals of recombinant kynureninase that diffracted to 2.0 A are obtained, and the atomic structure of the PLP-bound holoenzyme is determined by molecular replacement using the Pseudomonas fluorescens structure as the phasing model | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0283 | - |
3-hydroxy-DL-kynurenine | - |
Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-hydroxy-L-kynurenine + H2O | Homo sapiens | - |
3-hydroxyanthranilate + L-alanine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q16719 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using a Ni-CAM resin column | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| placenta | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.75 | - |
3-hydroxy-DL-kynurenine | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-hydroxy-DL-kynurenine + H2O | - |
Homo sapiens | 3-hydroxyanthranilate + DL-alanine | - |
? | |
| 3-hydroxy-L-kynurenine + H2O | - |
Homo sapiens | 3-hydroxyanthranilate + L-alanine | - |
? | |
| L-kynurenine + H2O | - |
Homo sapiens | anthranilate + L-alanine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| kynureninase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
activity assay | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.5 | - |
3-hydroxy-DL-kynurenine | - |
Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
activity assay | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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