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Literature summary for 3.7.1.3 extracted from

  • Walsh, H.A.; Botting, N.P.
    Purification and biochemical characterization of some of the properties of recombinant human kynureninase (2002), Eur. J. Biochem., 269, 2069-2074.
    View publication on PubMed
Search for more literature for 3.7.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Spodoptera frugiperda cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
3,7-dihydroxy-deamino-DL-kynurenine mixed inhibition Homo sapiens
D-kynurenine mixed inhibition Homo sapiens
L-kynurenine competitive inhibition, at low substrate concentrations; non-competitive inhibition, at higher levels of substrate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3
-
 3-hydroxy-L-kynurenine pH 7.9, at 37ºC Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52400
-
 recombinant enzyme, MALDI-TOF MS Homo sapiens
52500
-
 native enzyme, 2 * 52500, disc gel electrophoresis Homo sapiens
95000
-
 native enzyme, disc gel electrophoresis without SDS and mercaptoethanol Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxy-L-kynurenine + H2O Homo sapiens L-tryptophan metabolism, biosynthesis of nicotinamide nucleotides 3-hydroxyanthranilate + L-alanine
-
 ?
L-kynurenine + H2O Homo sapiens
-
 anthranilate + L-alanine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, ammonium sulfate precipitation, DEAE-Sepharose CL-6B affinity column, hydroxyapatite column, strong anion-exchange column at pH 8.6, strong cation-exchange column at pH 6.0 strong anion-exchange column at pH 6.0, 60fold Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.164
-
 pH 7.9, 37ºC Homo sapiens

Storage Stability

Storage Stability Organism
-80ºC, pyridoxal 5'-phosphate, more than 12 months, no loss of activity Homo sapiens
-80ºC, without pyridoxal 5'-phosphate, more than 12 months, no loss of activity Homo sapiens
4ºC, pyridoxal 5'-phosphate, up to 2 weeks, stable Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxy-L-kynurenine + H2O
-
 Homo sapiens 3-hydroxyanthranilate + L-alanine
-
 ?
3-hydroxy-L-kynurenine + H2O L-tryptophan metabolism, biosynthesis of nicotinamide nucleotides Homo sapiens 3-hydroxyanthranilate + L-alanine
-
 ?
L-kynurenine + H2O
-
 Homo sapiens anthranilate + L-alanine
-
 ?

Subunits

Subunits Comment Organism
homodimer native enzyme, 2 * 52500, disc gel electrophoresis Homo sapiens

Synonyms

Synonyms Comment Organism
L-kynurenine hydrolase
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0001
-
 3,7-dihydroxy-deamino-DL-kynurenine pH 7.9, at 37ºC Homo sapiens
0.012
-
 D-kynurenine pH 7.9, at 37ºC Homo sapiens
0.02
-
 L-kynurenine pH 7.9, at 37ºC, at low substrate concentrations Homo sapiens
0.055
-
 L-kynurenine pH 7.9, at 37ºC, at higher levels of substrate Homo sapiens