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3.7.1.3: kynureninase

This is an abbreviated version!
For detailed information about kynureninase, go to the full flat file.

Word Map on EC 3.7.1.3

Reaction

L-kynurenine
+
H2O
=
anthranilate
+
L-alanine

Synonyms

HsKynase, Kynase, KYNU, kynureninase, L-kynurenine hydrolase, PfKynase

ECTree

     3 Hydrolases
         3.7 Acting on carbon-carbon bonds
             3.7.1 In ketonic substances
                3.7.1.3 kynureninase

Storage Stability

Storage Stability on EC 3.7.1.3 - kynureninase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-78ºC, 0.1 mM potassium phosphate buffer, 0.2 mM pyridoxal 5'-phosphate, pH 8.0, 1 year, stable
-78ºC, 5 mM potassium phosphate buffer, 0.2 mM pyridoxal 5'-phosphate, pH 8.0, 1 month, loses 4% of activity
-80ºC, pyridoxal 5'-phosphate, more than 12 months, no loss of activity
-
-80ºC, without pyridoxal 5'-phosphate, more than 12 months, no loss of activity
-
4°C, phosphate buffer, pH 7.2, 0.02 mM pyridoxal-5'-phosphate, 1 week
-
4ºC, 5 mM potassium phosphate buffer, 0.2 mM pyridoxal 5'-phosphate, pH 8.0, 1 week, loses 40% of activity
4ºC, pyridoxal 5'-phosphate, up to 2 weeks, stable
-
70% saturated solution of ammonium sulfate containing 1 mM EDTA, 1 mM dithiothreitol, and 0.05 mM pyridoxal 5'-phosphate, 2 months
-
78°C, phosphate buffer, 1 year