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3.5.1.5: urease

This is an abbreviated version!
For detailed information about urease, go to the full flat file.

Word Map on EC 3.5.1.5

Reaction

Urea
+
H2O
=
CO2
 + 2 NH3

Synonyms

acid urease, Arthritogenic cationic 19 kDa antigen, BPU, canatoxin, embryo-specific soybean urease, Eu1, Eu4, HPU, jack bean urease, JBU, JBURE-II, More, PMU, urea amido hydrolase, Urea amidohydrolase, urease, urease JBURE-IIb, UreC

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.5 urease

Purification

Purification on EC 3.5.1.5 - urease

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
anion exchange chromatography, gel filtration
-
anion-exchange chromatography, hydrophobic interaction chromatography, affinity chromatography (Cellufine Sulphate)
-
commercial preparation
-
immobilized metal ion affinity chromatography (Ni2+), purified protein without enzymatic activity, recombinant Escherichia coli cells positive tested for urease activity in a urea segregation agar assay
large-scale HPLC-based
-
native enzyme 12.85fold from germinating seeds by acetone precipitation, anion exchange chromatography, and gel filtration
-
native enzyme 162fold from jack bean meal by immobilized metal affinity chromatography using Cu2+ chelated poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine methyl ester) [PHEMAH-Cu2+]-based cryogels, gel resin synthesis and method evaluation and optimization, first- and second-order kinetic constants for enzyme binding, overview. The enzyme can be repeatedly bound and eluted from (PHEMAH)-Cu2+ cryogel with less than 10% loss in column capacity
native enzyme by gel filtration and anion exchange chromatography
native urease 2.9fold by ethanol precipitation, and anion exchange chromatography
recombinant enzyme from Escherichia coli
-
recombinant enzyme from Lactobacilli by anion exchange and hydrophobic interaction chromatography, ultrafiltration and another, different step of anion exchange chromatography
recombinant His-tagged enzyme, refolded from Escherichia coli strain BL21(DE3) inclusion bodies, 1.75fold by nickel affinity chromatography
salt precipitation followed by ion exchange chromatography followed by gel filtration chromatography
-
using acetone precipitation, gel filtration and ion-exchange chromatography
-
using ammonium sulfate precipitation, dialysis, and anion exchange chromatography
-
using NH4(SO4)2 precipitation, phenyl-Sepharose column chromatography and gel filtration
-
wild-type and mutant enzymes C319A, C319S, C319D and C319Y
-