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3.5.1.5: urease

This is an abbreviated version!
For detailed information about urease, go to the full flat file.

Word Map on EC 3.5.1.5

Reaction

Urea
+
H2O
=
CO2
 + 2 NH3

Synonyms

acid urease, Arthritogenic cationic 19 kDa antigen, BPU, canatoxin, embryo-specific soybean urease, Eu1, Eu4, HPU, jack bean urease, JBU, JBURE-II, More, PMU, urea amido hydrolase, Urea amidohydrolase, urease, urease JBURE-IIb, UreC

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.5 urease

Temperature Stability

Temperature Stability on EC 3.5.1.5 - urease

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35
activity of free urease significantly decreases above 35°C
36
the purified recombinant enzyme is stable at pH 3.0-6.0 at temperatures below 50°C
4 - 50
-
1 h pH 8.0, stable between 4°C and 50°C
55
-
30 min, 15% loss of activity
65
-
stable up to
75
-
immobilized urease shows improved stability at 75°C. Soluble urease t1/2: 12 min, chitosan-immobilized urease t1/2: 58 min, alginate-immobilized urease t1/2: 43 min
77
-
15 min, soluble urease has a residual activity of 22%, alkylamine glass-immobilized enzyme and arylamine glass-immobilized urease retain 96% and 98%, respectively. Even after 90 min of incubation immobilized urease retains 50% of activity
additional information
-
Ni substantially increase stability of the intact metalloprotein, Tm = 79°C compared with apoenzyme Tm = 62°C