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3.5.1.5: urease

This is an abbreviated version!
For detailed information about urease, go to the full flat file.

Word Map on EC 3.5.1.5

Reaction

Urea
+
H2O
=
CO2
 + 2 NH3

Synonyms

acid urease, Arthritogenic cationic 19 kDa antigen, BPU, canatoxin, embryo-specific soybean urease, Eu1, Eu4, HPU, jack bean urease, JBU, JBURE-II, More, PMU, urea amido hydrolase, Urea amidohydrolase, urease, urease JBURE-IIb, UreC

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.5 urease

General Stability

General Stability on EC 3.5.1.5 - urease

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
agar-immobilized enzyme showes good mechanical stability
-
dithiothreitol, 50 mM, stabilizes
-
enhancement of stability of immobilised urease by biocompatible polymer-conjugated magnetic beads for the industrial application based on removal of urea significant increase in pH stability for urease-encapsulated magnetic beads compared with the free urease for both acidic and alkaline medium
interaction with silver nanoparticles result in destabilization of hexameric nature of the enzyme which is otherwise highly stable
loses activity irreversibly in the absence of NaCl
-
resistant to denaturation by 4 M urea
-
stability at 4°C is enhanced by addition of glycerol or 2-mercaptoethanol
-
the immobilized enzyme is more stable in wet condition than in semi-dry condition and storing it at 4°C is more favourable over 27°C. There is a tremendous decrease in activity when the immobilized urease is not stored in buffer (i.e. semi-dry condition) and storing it at 4°C improves the longevity. Both the alkyl and arylamine-urease in wet condition stored at 4°C are further reused (30times) and the residual activity are 40% and 20%, respectively
-
unstable during purification in absence of nickel. When 0.005 mM NiSO4 is present during growth and at 0.1 mM in buffers during sonication and purification, the urease is completely stable at room temperature during the purification procedure
bacterium strain SL100
-
urease encapsulated in chitosan nickel ferrite beads shows 50% loss of activity after 18 reuses
urease encapsulated in chitosan-coated alginate-magnetite shows 50% loss of activity after 14 reuses
urease encapsulated in chitosan-coated alginate-nickel ferrite beads shows 50% loss of activity after 14 reuses
urease encapsulated in chitosan-magnetite beads shows 50% loss of activity after 18 reuses