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3.5.1.5: urease

This is an abbreviated version!
For detailed information about urease, go to the full flat file.

Word Map on EC 3.5.1.5

Reaction

Urea
+
H2O
=
CO2
 + 2 NH3

Synonyms

acid urease, Arthritogenic cationic 19 kDa antigen, BPU, canatoxin, embryo-specific soybean urease, Eu1, Eu4, HPU, jack bean urease, JBU, JBURE-II, More, PMU, urea amido hydrolase, Urea amidohydrolase, urease, urease JBURE-IIb, UreC

ECTree

     3 Hydrolases
         3.5 Acting on carbon-nitrogen bonds, other than peptide bonds
             3.5.1 In linear amides
                3.5.1.5 urease

pH Stability

pH Stability on EC 3.5.1.5 - urease

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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.3
bacterium strain SL100
-
30 min, retains most of its activity
209170
2.5
-
the amount of viable Yersinia enterocolitica biovar 1A decreases significantly when incubated at pH 2.5 for 2 h. No decrease is observed at this pH in the presence of urea
711606
3 - 6
the purified recombinant enzyme is stable at pH 3.0-6.0 at temperatures below 50°C
733196
3 - 8
3 - 9
-
37°C, 30 min, stable
209166
3.5
-
22°C, 12 h, soluble enzyme retains 5% of its original activity, alkylamine glass-immobilized enzyme and arylamine glass immobilized urease retain 37% and 38%, respectively
688429
4
-
enzyme gets irreversibly inactivated at pH 4
712722
4 - 6
4 - 8
6 - 10
-
30°C, 2 h, stable
209167
7
-
in presence of 10 mM EDTA protein-nickel binding remains intact
209154
7 - 10
-
30°C, 5 h, stable
209155
7 - 9
-
1 h 30°C, stable within the pH range of 7.0-9.0
754683
additional information
a significant increase in pH stability is observed for urease-encapsulated magnetic beads compared with the free urease for both acidic and alkaline medium
752526