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3.4.22.15: cathepsin L

This is an abbreviated version!
For detailed information about cathepsin L, go to the full flat file.

Word Map on EC 3.4.22.15

Reaction

similar to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity towards protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity =

Synonyms

AgCatL, Aldrichina grahami cysteine proteinase, cat L, Cat L-A, Cath L, cath-L, cathepsin L, cathepsin L isoform CRA-b, cathepsin L-A, cathepsin L-A1, cathepsin L-A2, cathepsin L-A3, cathepsin L-B, cathepsin L-like, cathepsin L-like cysteine protease, cathepsin L-like enzyme, cathepsin L-like protease, cathepsin L-like protein, cathepsin L-like proteinase, cathepsin L-like rCPB2.8, cathepsin L1, cathepsin L1H, cathepsin L3, cathepsin-L, cathepsin-L T2V, CathL, CatL, CATL A IV, CATL-1, CATL-2, CatL1G, CatL1H, CatL5, CL1, CL3, CL41.5, CPL, cpl-1, CsCPL, CsCPL-m, CtL, CTSL, CTSL1, CTSL2, Cwp84, FhCL1, FhCL3, Har-CatL, human cathepsin L, major excreted protein, MEP, PDP, progesterone-dependent protein, rhodesain, SMCL1, SoCatL, sperm-histone protease, TsolCL

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.22 Cysteine endopeptidases
                3.4.22.15 cathepsin L

Activating Compound

Activating Compound on EC 3.4.22.15 - cathepsin L

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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
7beta-hydroxycholesterol
-
significantly enhances cathepsin L in cells from healthy donors but not in cells from coronary artery disease patients
Brij 35
329.61% relative activity at 0.01% (v/v)
CoA
-
activates
cystatin
-
activates
-
cysteamine
-
strong activation of hydrolysis of insulin or albumin, at pH 4.0 and at pH 5.5
cysteine
dibutyryl-cAMP
-
treatment of cells for identification of formerly N-linked glycopeptides. Treatment results in upregulation of follistatin-related protein 1, cathepsin L, and neuroblastoma suppressor of tumorigenicity, and the downregulation of tenascin C and cationin-dependent mannose-6-phosphate receptor
dithiothreitol
EGTA
about 3fold activation at 5 mM
glutathione
-
strong activation of hydrolysis of insulin or albumin, at pH 4.0 and at pH 5.5
heparin sulfate
-
heparin sulfate (0.02 mg/ml) has no detectable effect on the rate of hydrolysis of the substrate at 0.0025 mM while it increases by 10fold the rate of substrate hydrolysis at 0.05 mM
L-cysteine
-
114% activity at 5 mM
protegrin-3
-
a porcine cathelicidin, molecular weight 11.7 kDa. The cathelin-like domain efficiently activates human cathepsin L. Partial deletion of the L2 loop of cathelin-like domain, a structurally equivalent region important ininteraction of cystatins with proteases, significantly decreases its activating effect on cathepsin L. Proposal of a complex model based on this functional loop, with the cathelin-like domain fitting into the active cleft of the enzyme in an analogous way as cystatin B does
-
proteinase inhibitor for serine protease
-
113% activity at 0.1 g/l
-
SDS
201.87% relative activity at 0.01% (v/v)
Soybean trypsin inhibitor
-
activates
-
thiol-reducing agent
-
thiol-reducing reagent
Agama stellio stellio
-
required for activation
-
Triton X-100
208.82% relative activity at 0.05% (v/v)
Tween 20
337.68% relative activity at 0.01% (v/v)
Urea
-
enhances hydrolysis of azocasein
additional information
-