3.4.22.15: cathepsin L

This is an abbreviated version!
For detailed information about cathepsin L, go to the full flat file.

Reaction

similar to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity towards protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity =

Synonyms

AgCatL, Aldrichina grahami cysteine proteinase, cat L, Cat L-A, Cath L, cath-L, cathepsin L, cathepsin L isoform CRA-b, cathepsin L-A, cathepsin L-A1, cathepsin L-A2, cathepsin L-A3, cathepsin L-B, cathepsin L-like, cathepsin L-like cysteine protease, cathepsin L-like enzyme, cathepsin L-like protease, cathepsin L-like protein, cathepsin L-like proteinase, cathepsin L-like rCPB2.8, cathepsin L1, cathepsin L1H, cathepsin L3, cathepsin-L, cathepsin-L T2V, CathL, CatL, CATL A IV, CATL-1, CATL-2, CatL1G, CatL1H, CatL5, CL1, CL3, CL41.5, CPL, cpl-1, CsCPL, CsCPL-m, CtL, CTSL, CTSL1, CTSL2, Cwp84, FhCL1, FhCL3, Har-CatL, human cathepsin L, major excreted protein, MEP, PDP, progesterone-dependent protein, rhodesain, SMCL1, SoCatL, sperm-histone protease, TsolCL

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.22 Cysteine endopeptidases

                3.4.22.15 cathepsin L

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Results	in table
49	Activating Compound
486	AA Sequence
29	Application
1	CAS Registry Number
54	Cloned(Commentary)
17	Crystallization (Commentary)
2773	Disease/ Diagnostics
18	Expression
55	General Information
5	General Stability
270	IC50 Value
1005	Inhibitors
40	kcat/KM [mM/s]
218	Ki Value [mM]
116	KM Value [mM]
75	Localization
3	Metals/Ions
81	Molecular Weight [Da]
83	Natural Substrates/ Products (Substrates)
267	Organism
90	PDB ID
61	pH Optimum
28	pH Range
19	pH Stability
7	pI Value
26	Posttranslational Modification
27	Protein Variants
60	Purification (Commentary)
1	Reaction
9	Reaction Type
263	Reference
3	Renatured (Commentary)
301	Source Tissue
25	Specific Activity [micromol/min/mg]
3	Storage Stability
478	Substrates and Products (Substrate)
62	Subunits
160	Synonyms
24	Temperature Optimum [°C]
6	Temperature Range [°C]
14	Temperature Stability [°C]
114	Turnover Number [1/s]

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RENATURED/Commentary

ORGANISM

UNIPROT

LITERATURE

recombinant His6-tagged mature domain of CsCPL (CsCPL-m) solubilized from Escherichia coli inclusion bodies by dialysis through stepwise dilution of urea (6 to 1 M) in 20 mM Tris-HCl and 5 mM EDTA buffer (pH 8.0). Finally, the urea is removed by dialysis using phosphate-buffered saline, pH 7.4

Clonorchis sinensis

B5G4Y8

754985

the optimum cathepsin L renaturation buffer consists of 50 mM Tris, 4 mM GSH, 0.5 mM GSSG, 300 mM MgSO4, 0.05% (w/v) 3-(3-cholamidopropyl)-dimethylammonio-1-propane sulfonate, 0.04 mM propetide, at pH 7.75 and 16°C

Homo sapiens

-

711345

using 100 mM Tris-HCl, pH 8.0, 1 mM EDTA, 20% (v/v) glycerol, 250 mM L-arginine, 2 mM reduced glutathione, 1 mM oxidized glutathione

Toxoplasma gondii

Q6DMN0

712384