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Literature summary for 3.4.22.15 extracted from

  • Zhu, B.W.; Zhao, L.L.; Sun, L.M.; Li, D.M.; Murata, Y.; Yu, L.; Zhang, L.
    Purification and characterization of a cathepsin L-like enzyme from the body wall of the sea cucumber Stichopus japonicus (2008), Biosci. Biotechnol. Biochem., 72, 1430-1437.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 1 mM, 110% of initial activity Apostichopus japonicus
EDTA 1 mM, 106% of initial activity Apostichopus japonicus

Inhibitors

Inhibitors Comment Organism Structure
1- (L-trans-epoxysuccinyl-leucylamino)-4-guanidinobutane i.e. E-64, 0.1 mM, complete loss of activity Apostichopus japonicus
antipain 1 mM, complete loss of activity Apostichopus japonicus
Cu2+ 1 mM, 19% residual activity Apostichopus japonicus
iodoacetic acid 1 mM, complete loss of activity Apostichopus japonicus
leupeptin 1 mM, 31% residual activity Apostichopus japonicus
Zn2+ 1 mM, complete loss of activity Apostichopus japonicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.069
-
N-carbobenzoxy-L-phenylalanine-L-arginine 7-amido-4-methylcoumarin pH 5.5, 37°C Apostichopus japonicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
x * 63000, SDS-PAGE Apostichopus japonicus

Organism

Organism UniProt Comment Textmining
Apostichopus japonicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from body wall Apostichopus japonicus

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
59.9
-
pH 5.5, 37°C Apostichopus japonicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine 7-amido-4-methylcoumarin + H2O very poor substrate Apostichopus japonicus ?
-
?
N-carbobenzoxy-L-arginine-L-arginine 7-amido-4-methylcoumarin + H2O 7% of the activity with N-carbobenzoxy-L-phenylalanine-L-arginine 7-amido-4-methylcoumarin Apostichopus japonicus ?
-
?
N-carbobenzoxy-L-phenylalanine-L-arginine 7-amido-4-methylcoumarin + H2O
-
Apostichopus japonicus ?
-
?

Subunits

Subunits Comment Organism
? x * 63000, SDS-PAGE Apostichopus japonicus

Synonyms

Synonyms Comment Organism
cathepsin L-like enzyme
-
Apostichopus japonicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Apostichopus japonicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
stable below Apostichopus japonicus
55
-
10 min, 55% residual activity Apostichopus japonicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12.8
-
N-carbobenzoxy-L-phenylalanine-L-arginine 7-amido-4-methylcoumarin pH 5.5, 37°C Apostichopus japonicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Apostichopus japonicus

pH Range

pH Minimum pH Maximum Comment Organism
5
-
rapid decrease in activity above Apostichopus japonicus