Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 1 mM, 110% of initial activity | Apostichopus japonicus | |
EDTA | 1 mM, 106% of initial activity | Apostichopus japonicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1- (L-trans-epoxysuccinyl-leucylamino)-4-guanidinobutane | i.e. E-64, 0.1 mM, complete loss of activity | Apostichopus japonicus | |
antipain | 1 mM, complete loss of activity | Apostichopus japonicus | |
Cu2+ | 1 mM, 19% residual activity | Apostichopus japonicus | |
iodoacetic acid | 1 mM, complete loss of activity | Apostichopus japonicus | |
leupeptin | 1 mM, 31% residual activity | Apostichopus japonicus | |
Zn2+ | 1 mM, complete loss of activity | Apostichopus japonicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.069 | - |
N-carbobenzoxy-L-phenylalanine-L-arginine 7-amido-4-methylcoumarin | pH 5.5, 37°C | Apostichopus japonicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
x * 63000, SDS-PAGE | Apostichopus japonicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Apostichopus japonicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
from body wall | Apostichopus japonicus |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
59.9 | - |
pH 5.5, 37°C | Apostichopus japonicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine 7-amido-4-methylcoumarin + H2O | very poor substrate | Apostichopus japonicus | ? | - |
? | |
N-carbobenzoxy-L-arginine-L-arginine 7-amido-4-methylcoumarin + H2O | 7% of the activity with N-carbobenzoxy-L-phenylalanine-L-arginine 7-amido-4-methylcoumarin | Apostichopus japonicus | ? | - |
? | |
N-carbobenzoxy-L-phenylalanine-L-arginine 7-amido-4-methylcoumarin + H2O | - |
Apostichopus japonicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 63000, SDS-PAGE | Apostichopus japonicus |
Synonyms | Comment | Organism |
---|---|---|
cathepsin L-like enzyme | - |
Apostichopus japonicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Apostichopus japonicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable below | Apostichopus japonicus |
55 | - |
10 min, 55% residual activity | Apostichopus japonicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.8 | - |
N-carbobenzoxy-L-phenylalanine-L-arginine 7-amido-4-methylcoumarin | pH 5.5, 37°C | Apostichopus japonicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Apostichopus japonicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | - |
rapid decrease in activity above | Apostichopus japonicus |