3.4.22.15: cathepsin L

This is an abbreviated version!
For detailed information about cathepsin L, go to the full flat file.

Reaction

similar to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity towards protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity =

Synonyms

AgCatL, Aldrichina grahami cysteine proteinase, cat L, Cat L-A, Cath L, cath-L, cathepsin L, cathepsin L isoform CRA-b, cathepsin L-A, cathepsin L-A1, cathepsin L-A2, cathepsin L-A3, cathepsin L-B, cathepsin L-like, cathepsin L-like cysteine protease, cathepsin L-like enzyme, cathepsin L-like protease, cathepsin L-like protein, cathepsin L-like proteinase, cathepsin L-like rCPB2.8, cathepsin L1, cathepsin L1H, cathepsin L3, cathepsin-L, cathepsin-L T2V, CathL, CatL, CATL A IV, CATL-1, CATL-2, CatL1G, CatL1H, CatL5, CL1, CL3, CL41.5, CPL, cpl-1, CsCPL, CsCPL-m, CtL, CTSL, CTSL1, CTSL2, Cwp84, FhCL1, FhCL3, Har-CatL, human cathepsin L, major excreted protein, MEP, PDP, progesterone-dependent protein, rhodesain, SMCL1, SoCatL, sperm-histone protease, TsolCL

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.22 Cysteine endopeptidases

                3.4.22.15 cathepsin L

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
49	Activating Compound
486	AA Sequence
29	Application
1	CAS Registry Number
54	Cloned(Commentary)
17	Crystallization (Commentary)
2773	Disease/ Diagnostics
18	Expression
55	General Information
5	General Stability
270	IC50 Value
1005	Inhibitors
40	kcat/KM [mM/s]
218	Ki Value [mM]
116	KM Value [mM]
75	Localization
3	Metals/Ions
81	Molecular Weight [Da]
83	Natural Substrates/ Products (Substrates)
267	Organism
90	PDB ID
61	pH Optimum
28	pH Range
19	pH Stability
7	pI Value
26	Posttranslational Modification
27	Protein Variants
60	Purification (Commentary)
1	Reaction
9	Reaction Type
263	Reference
3	Renatured (Commentary)
301	Source Tissue
25	Specific Activity [micromol/min/mg]
3	Storage Stability
478	Substrates and Products (Substrate)
62	Subunits
160	Synonyms
24	Temperature Optimum [°C]
6	Temperature Range [°C]
14	Temperature Stability [°C]
114	Turnover Number [1/s]

Engineering

print visible entries

print all entries

Go back to the abbreviated version
of the Enzyme Summary Page.

Engineering on EC 3.4.22.15 - cathepsin L

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to Protein Variants Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

L69W

Fasciola hepatica

-

isoform CatL5 mutant, compared to the wild type enzyme the mutant has a higher efficiency of cleavage (kcat) against tosyl-Gly-L-Pro-L-Arg-7-amido-4-methylcoumarin

717293

A205L

Homo sapiens

-

mutant shows an effective cathepsin K-like preference for Leu and Pro

682739

C25A

Homo sapiens

P07711

inactive

718157

C25S

2 entries

C25S/S24A/W26Y

Homo sapiens

P07711

mutant constructed to confer silica condensing activity to cathepsin L. Mutant displays significant condensing activity

697291

C25S/S24A/W26Y/M161L/D162N/G164A/V165M

Homo sapiens

P07711

mutant constructed to confer silica condensing activity to cathepsin L. Mutant displays significant condensing activity, but less than mutant C25S/S24A/W26Y

697291

G139R

2 entries

L67Y/A205L

Homo sapiens

-

mutation induces a switch of the enzymatic specificity toward small selective inhibitors and peptidyl substrates

682739

T223V

2 entries

T223V/C138S

Homo sapiens

P07711

the mutations prevent catalytic activity

677987

T223V/C138S/K99A/K104A

Homo sapiens

P07711

the mutations disrupt the putative heparin binding site

677987

T223V/DELTAE286-E289

Homo sapiens

P07711

the mutation shows reduced enzyme activity

682723

T223V/DELTAE286-N293

Homo sapiens

P07711

the mutation shows reduced enzyme activity

682723

T223V/M274L/D275N/G277A/V278M

Homo sapiens

P07711

the mutation shows reduced enzyme activity

682723

C143A

Sciaenops ocellatus

A0A0K0M968

site-directed mutagenesis

753596

additional information

12 entries

C25S

Homo sapiens

P07711

mutant constructed to confer silica condensing activity to cathepsin L. Mutation does not display significant condensing activity

697291

C25S

Homo sapiens

P07711

site-directed mutagenesis, almost inactive mutant that still performs autocatalytic cleavage

753565

G139R

Homo sapiens

P07711

2fold decrease in binding affinity to the inhibitory fragment p41 of major histocompatibility complex class II-associated invariant chain

698753

G139R

Homo sapiens

P07711

KI-value increased compared to wild-type

698753

T223V

Homo sapiens

P07711

the mutation removes the potential glycosylation site

677987

T223V

Homo sapiens

P07711

the mutation removes the potential glycosylation site and shows reduced enzyme activity

682723

additional information

Clostridioides difficile

Q83UF3

expression of the mature protein, amino acids 92-518, does not give an active protein. A protein fragment, amino acids 92-518, does not display proteolytic activity. A fragment including the propeptide, amino acids 30-518, displays proteolytic activity on azocasein and fibronectin and can be processed to the active mature protein

727753

additional information

Clostridioides difficile

-

expression of the mature protein, amino acids 92-518, does not give an active protein. A protein fragment, amino acids 92-518, does not display proteolytic activity. A fragment including the propeptide, amino acids 30-518, displays proteolytic activity on azocasein and fibronectin and can be processed to the active mature protein

727753

additional information

Homo sapiens

-

cathepsin L inhibition by siRNA triggers autophagy

696405

additional information

Homo sapiens

-

constitutive cardiac over-expression in mice attenuates the hypertropic response, markedly reduces apoptosis, and fibrosis. Cardiac function is also preserved in hearts with increased cathepsin L levels in response to hypertropic stimuli, associated with attenuation of the Akt/GSK3beta signaling cascade

699624

additional information

Homo sapiens

-

knock-down of cathepsin L by siRNA results in a marked decrease in the level of the processed form of heparanase by 65%. Cathepsin L knock-out fibroblasts express high levels of the full-length 65-kDa proheparanase and display almost no heparanase enzymatic activity

698768

additional information

Homo sapiens

P07711

mutant C25S/S24A/W26Y/M161L/D162N/G164A/V165M plus 173ESTESDNN180 to ISNNQ and mutant C25S/S24A/W26Y/M161L/D162N/G164A/V165M/E159S/D160S plus 173ESTESDNN180 to ISNNQ and mutant C25S/S24A/W26Y/M161L/D162N/G164A/V165M/E159S/D160S/E153S/P154S plus 173ESTESDNN180 to ISNNQ increasingly match the features that are unique silicatein alpha. The additional mutations on mutant C25S/S24A/W26Y of cathepsin L to further increase its resemblance to silicatein alpha do not significantly increase the ability to condense silica

697291

additional information

Mus musculus

-

cathepsin L knock-out mice show major decreases in adrenocorticotropic hormone, beta-endorphin, and alpha-melanocyte stimulating hormone in pituitary, accompanied by increased levels of proopiomelanocortin

681531

additional information

Mus musculus

P06797

cathepsin L knock-out mice show major decreases in adrenocorticotropic hormone, beta-endorphin, and alpha-melanocyte stimulating hormone in pituitary, accompanied by increased levels of proopiomelanocortin

681531

additional information

Mus musculus

-

construction of cell lines with reduced expression of cathepsin L by stable transfection of H-59 cells with a plasmid expressing an antisense fragment of mouse cathepsin L cDNA. Transfected cells show a loss of IGF-I-induced receptor phosphorylation and Shc recruitment, reduced IGF-I (but not IGFII)-induced cellular proliferation and migration, decreased anchorage-independent growth and reduced plasma membrane levels of IGF type I receptor. Changes result in apoptosis in vivo and an impaired ability to form liver metastases

700521

additional information

Mus musculus

-

mice lacking the activity of cathepsin B, cathepsin L, or cathepsin S are similar to wild-type in their ability to control the growth and dissemination of Mycobacterium tuberculosis

699405

additional information

Sus scrofa

E1UJ48

the CTSL polymorphism C143T in exon 5 is associated with average daily gain, weight of lean cuts (LC) and back fat thickness estimated breeding values

709799

additional information

Sus scrofa

-

the CTSL polymorphism C143T in exon 5 is associated with average daily gain, weight of lean cuts (LC) and back fat thickness estimated breeding values

709799