Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | 0.3 mM, slight activation | Oryctolagus cuniculus | |
EDTA | essential for activity | Oryctolagus cuniculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antipain | - |
Oryctolagus cuniculus | |
iodoacetamide | - |
Oryctolagus cuniculus | |
iodoacetic acid | strong | Oryctolagus cuniculus | |
L-1-tosyl-2-phenylethylchloromethylketone | - |
Oryctolagus cuniculus | |
leupeptin | - |
Oryctolagus cuniculus | |
additional information | no inhibition by pepstatin, phenylmethane sulfonyl fluoride | Oryctolagus cuniculus | |
tosyl-Lys-CH2Cl | - |
Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
gel filtration | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | skeletal muscle myofibrillar enzyme | Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme removes the Ca-sensitivity of ATPase activity of myofibrils | Oryctolagus cuniculus | ? | - |
? | |
myosin + H2O | - |
Oryctolagus cuniculus | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
57 | - |
pH 6.0, 10 min, 50% loss of activity | Oryctolagus cuniculus |
70 | - |
pH 6.0, 10 min, complete loss of activity | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.1 | - |
hydrolysis of myosin | Oryctolagus cuniculus |
7 | - |
removal of the Ca-sensitivity of ATPase activity of myofibrils | Oryctolagus cuniculus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 6.5 | 37°C, 1 h, stable | Oryctolagus cuniculus |
7.5 | - |
37°C, 1 h, unstable above | Oryctolagus cuniculus |