3.4.22.15: cathepsin L

This is an abbreviated version!
For detailed information about cathepsin L, go to the full flat file.

Reaction

similar to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity towards protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity =

Synonyms

AgCatL, Aldrichina grahami cysteine proteinase, cat L, Cat L-A, Cath L, cath-L, cathepsin L, cathepsin L isoform CRA-b, cathepsin L-A, cathepsin L-A1, cathepsin L-A2, cathepsin L-A3, cathepsin L-B, cathepsin L-like, cathepsin L-like cysteine protease, cathepsin L-like enzyme, cathepsin L-like protease, cathepsin L-like protein, cathepsin L-like proteinase, cathepsin L-like rCPB2.8, cathepsin L1, cathepsin L1H, cathepsin L3, cathepsin-L, cathepsin-L T2V, CathL, CatL, CATL A IV, CATL-1, CATL-2, CatL1G, CatL1H, CatL5, CL1, CL3, CL41.5, CPL, cpl-1, CsCPL, CsCPL-m, CtL, CTSL, CTSL1, CTSL2, Cwp84, FhCL1, FhCL3, Har-CatL, human cathepsin L, major excreted protein, MEP, PDP, progesterone-dependent protein, rhodesain, SMCL1, SoCatL, sperm-histone protease, TsolCL

ECTree

     3 Hydrolases

         3.4 Acting on peptide bonds (peptidases)

             3.4.22 Cysteine endopeptidases

                3.4.22.15 cathepsin L

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Results	in table
49	Activating Compound
486	AA Sequence
29	Application
1	CAS Registry Number
54	Cloned(Commentary)
17	Crystallization (Commentary)
2773	Disease/ Diagnostics
18	Expression
55	General Information
5	General Stability
270	IC50 Value
1005	Inhibitors
40	kcat/KM [mM/s]
218	Ki Value [mM]
116	KM Value [mM]
75	Localization
3	Metals/Ions
81	Molecular Weight [Da]
83	Natural Substrates/ Products (Substrates)
267	Organism
90	PDB ID
61	pH Optimum
28	pH Range
19	pH Stability
7	pI Value
26	Posttranslational Modification
27	Protein Variants
60	Purification (Commentary)
1	Reaction
9	Reaction Type
263	Reference
3	Renatured (Commentary)
301	Source Tissue
25	Specific Activity [micromol/min/mg]
3	Storage Stability
478	Substrates and Products (Substrate)
62	Subunits
160	Synonyms
24	Temperature Optimum [°C]
6	Temperature Range [°C]
14	Temperature Stability [°C]
114	Turnover Number [1/s]

pH Stability

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pH Stability on EC 3.4.22.15 - cathepsin L

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pH STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

3.5 - 6.6

Haliotis discus hannai

K7QTY9

over 50% activity remaining, profile overview

753600

3.6 - 6.2

Homo sapiens

-

activity of cathepsin L increases from pH 3.6 to 6.2 and is independent of the calcium concentration, The rate of trypsin inactivation by cathepsin L is thus approximately 10 times faster at pH 4.0 than at pH 5.5

712089

4 - 7

Gadus chalcogrammus

-

at pH 4.0 and 5.0 activity decreases slowly and remains at 38.86% and 48.54%, respectively, after incubation at 45°C for 120 min, at pH 7.0, activity of cathepsin L decreases sharply to about 10% residual activity after incubation at 45°C for 10 min

679950

4 - 9

2 entries

4.5 - 5.5

Homo sapiens

-

37°C, 24 h, more than 70% of the activity is retained

647919

4.5 - 6.5

Oryctolagus cuniculus

-

37°C, 1 h, stable

647929

5

Gallus gallus

-

40 min, 10% loss of activity

647903

5 - 6.5

Cyprinus carpio

-

stable

647927

5 - 8.5

Misgurnus mizolepis

A5HJW4

the enzyme remains stable at neutral and alkaline pH (8.5), its activity is greatly decreased at pH 5.0 (lysosomal pH) and below

710915

5.5 - 6

Ovis aries

-

maximally stable

647943

7

3 entries

7.2 - 7.4

Rattus norvegicus

-

37°C, stable

647918

7.4

Gallus gallus

-

2 min, 97% loss of activity

647912

7.5

Oryctolagus cuniculus

-

37°C, 1 h, unstable above

647929

8

2 entries

4 - 9

Fasciola gigantica

-

after incubation for 2 h at 37°C in AMT buffers (100 mM sodium acetate, 100 mM MES, 200 mM Tris, 1 mM EDTA), the isoform CatL1G shows residual activities of about 30% at pH 4.0, about 75% at pH 5.0, about 85% at pH 6.0, about 45% at pH 7.0, and about 10% at pH 8.0-9.0. Isoform CatL1G retains 25% activity after 2 h at pH 7.5, but retains over 80% activity over 4 h at pH 5.5 and 50% over 24 h

717293

4 - 9

Fasciola hepatica

-

isoform CatL5 undergoes significant autoprocessing if the pH of the yeast media is allowed to drop below pH 6.0. After incubation for 2 h at 37°C in AMT buffers (100 mM sodium acetate, 100 mM MES, 200 mM Tris, 1 mM EDTA), the isoform CatL5 shows residual activities of about 30% at pH 4.0-5.0, about 60% at pH 6.0, about 85% at pH 7.0, about 100% at pH 8.0, and about 60% at pH 9.0. Isoform CatL5 and mutant CatL5 L69W retain more than 90% activity after 4 h incubation at pH 7.5 and about 40% activity after incubation for 24 h

717293

7

Gallus gallus

-

10 min, 97% loss of activity

647903

7

Homo sapiens

-

15 min, 85% loss of activity

647919

7

Priacanthus macracanthus

-

at 50°C, the enzyme remains stable at pH 7.0

712879

8

Homo sapiens

P07711

the mature form of cathepsin L exhibits very little activity at pH 8.0 and is largely unfolded

677987

8

Rattus norvegicus

-

40°C, 60 min, stable

647918