Activating Compound | Comment | Organism | Structure |
---|---|---|---|
heparin sulfate | heparin sulfate (0.02 mg/ml) has no detectable effect on the rate of hydrolysis of the substrate at 0.0025 mM while it increases by 10fold the rate of substrate hydrolysis at 0.05 mM | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-Phe-L-Arg-7-amido-4-methylcoumarin | the susceptibility of recombinant enzyme to substrate inhibition at 25°C is partially reversible, i.e., it is absent at 37°C and is displayed when the same enzyme sample is assayed again at 25°C | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00033 | - |
L-Phe-L-Arg-7-amido-4-methylcoumarin | in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 37°C | Trypanosoma brucei | |
0.00037 | - |
L-Phe-L-Arg-7-amido-4-methylcoumarin | in the presence of 0.01 mg/ml heparin sulfate, in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C | Trypanosoma brucei | |
0.00041 | - |
L-Phe-L-Arg-7-amido-4-methylcoumarin | in the presence of 0.005 mg/ml heparin sulfate, in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C | Trypanosoma brucei | |
0.00044 | - |
L-Phe-L-Arg-7-amido-4-methylcoumarin | in the presence of 0.001 mg/ml heparin sulfate, in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C | Trypanosoma brucei | |
0.00046 | - |
L-Phe-L-Arg-7-amido-4-methylcoumarin | in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C | Trypanosoma brucei | |
0.00047 | - |
L-Phe-L-Arg-7-amido-4-methylcoumarin | in the presence of 0.02 mg/ml heparin sulfate, in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C | Trypanosoma brucei | |
0.00695 | - |
L-Val-L-Leu-L-Arg-7-amido-4-methylcoumarin | in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 37°C | Trypanosoma brucei | |
0.01242 | - |
L-Val-L-Leu-L-Arg-7-amido-4-methylcoumarin | in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C | Trypanosoma brucei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fibrinogen + H2O | Trypanosoma brucei | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fibrinogen + H2O | - |
Trypanosoma brucei | ? | - |
? | |
L-Phe-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Trypanosoma brucei | L-Phe-L-Arg + 7-amino-4-methylcoumarin | - |
? | |
L-Val-L-Leu-L-Arg-7-amido-4-methylcoumarin + H2O | - |
Trypanosoma brucei | L-Val-L-Leu-L-Arg + 7-amino-4-methylcoumarin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CatL | - |
Trypanosoma brucei |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 37 | the activity of CATL decays at substrate concentrations higher than 0.01 mM, in a concentration-dependent manner. The activity of CATL is unaltered at saturation concentrations of substrate when the protease is assayed at 37°C | Trypanosoma brucei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.39 | - |
L-Phe-L-Arg-7-amido-4-methylcoumarin | in the presence of 0.01 mg/ml heparin sulfate, in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C | Trypanosoma brucei | |
0.43 | - |
L-Phe-L-Arg-7-amido-4-methylcoumarin | in the presence of 0.02 mg/ml heparin sulfate, in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C | Trypanosoma brucei | |
0.45 | - |
L-Phe-L-Arg-7-amido-4-methylcoumarin | in the presence of 0.005 mg/ml heparin sulfate, in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C | Trypanosoma brucei | |
0.52 | - |
L-Phe-L-Arg-7-amido-4-methylcoumarin | in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C | Trypanosoma brucei | |
0.52 | - |
L-Phe-L-Arg-7-amido-4-methylcoumarin | in the presence of 0.001 mg/ml heparin sulfate, in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C | Trypanosoma brucei | |
0.89 | - |
L-Val-L-Leu-L-Arg-7-amido-4-methylcoumarin | in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C | Trypanosoma brucei | |
1.01 | - |
L-Phe-L-Arg-7-amido-4-methylcoumarin | in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 37°C | Trypanosoma brucei | |
1.07 | - |
L-Val-L-Leu-L-Arg-7-amido-4-methylcoumarin | in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 37°C | Trypanosoma brucei |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05241 | - |
L-Phe-L-Arg-7-amido-4-methylcoumarin | in 50mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C | Trypanosoma brucei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
71.6 | - |
L-Val-L-Leu-L-Arg-7-amido-4-methylcoumarin | in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C | Trypanosoma brucei | |
155.3 | - |
L-Val-L-Leu-L-Arg-7-amido-4-methylcoumarin | in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 37°C | Trypanosoma brucei | |
999.8 | - |
L-Phe-L-Arg-7-amido-4-methylcoumarin | in the presence of 0.02 mg/ml heparin sulfate, in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C | Trypanosoma brucei | |
1088 | - |
L-Phe-L-Arg-7-amido-4-methylcoumarin | in the presence of 0.01 mg/ml heparin sulfate, in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C | Trypanosoma brucei | |
1119 | - |
L-Phe-L-Arg-7-amido-4-methylcoumarin | in the presence of 0.001 mg/ml heparin sulfate, in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C | Trypanosoma brucei | |
1121 | - |
L-Phe-L-Arg-7-amido-4-methylcoumarin | in the presence of 0.005 mg/ml heparin sulfate, in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C | Trypanosoma brucei | |
1187 | - |
L-Phe-L-Arg-7-amido-4-methylcoumarin | in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 25°C | Trypanosoma brucei | |
3148 | - |
L-Phe-L-Arg-7-amido-4-methylcoumarin | in 50 mM Na2HPO4, pH 6.5, 100 mM NaCl, 5 mM EDTA, 2.5 mM dithiothreitol, at 37°C | Trypanosoma brucei |