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3.4.21.118: kallikrein 8

This is an abbreviated version!
For detailed information about kallikrein 8, go to the full flat file.

Word Map on EC 3.4.21.118

Reaction

cleavage of amide substrates following the basic amino acids Arg or Lys at the P1 position, with a preference for Arg over Lys =

Synonyms

Alzheimers associated protease, brain serine protease 1, GK-8, hK8, kallikrein 8, kallikrein 8 protease, kallikrein-8, kallikrein-related peptidase, kallikrein-related peptidase 8, kallikrein-related peptidase-8, KLK8, KRP/hK8, mK8, neuropsin, NP, ovasin, P1 kallikrein, PRSS19, RGK-8, S01.244, TADG-14, TADG14, tissue kallikrein, type I neuropsin, type II neuropsin

ECTree

     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.118 kallikrein 8

KM Value

KM Value on EC 3.4.21.118 - kallikrein 8

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.4312
benzyloxycarbonyl-Gly-Gly-Arg-7-amido-4-methylcoumarin
-
in 100 mM sodium phosphate, 0.01% (v/v) Tween 20, pH 8.5, at 37°C
0.07
benzyloxycarbonyl-L-Val-L-Val-L-Arg-7-amido-4-methylcoumarin
37°C, pH 8.0
0.23 - 0.28
D-Val-Leu-Arg-4-nitroanilide
0.07
L-Pro-L-Phe-L-Arg-7-amido-4-methylcoumarin
37°C, pH 8.0
8.36
Pro-Phe-Arg-4-methylcoumaryl-7-amide
recombinant wild-type enzyme, pH 8.0, 25°C
0.1533
Pro-Phe-Arg-7-amido-4-methylcoumarin
-
in 100 mM sodium phosphate, 0.01% (v/v) Tween 20, pH 8.5, at 37°C
0.1
t-butyloxycarbonyl-L-Leu-L-Lys-L-Arg-7-amido-4-methylcoumarin
37°C, pH 8.0
0.07
t-butyloxycarbonyl-L-Phe-L-Ser-L-Arg-7-amido-4-methylcoumarin
37°C, pH 8.0
0.1
t-butyloxycarbonyl-L-Val-L-Leu-L-Lys-7-amido-4-methylcoumarin
37°C, pH 8.0
0.07
t-butyloxycarbonyl-L-Val-L-Pro-L-Arg-7-amido-4-methylcoumarin
37°C, pH 8.0
0.32
tert-butyloxycarbonyl-Asp(benzyloxy)-Pro-Arg-4-methylcoumaryl-7-amide
recombinant wild-type enzyme, pH 8.0, 25°C
0.34
tert-butyloxycarbonyl-Asp-Pro-Arg-4-methylcoumaryl-7-amide
recombinant enzyme, pH 8.0, 37°C
0.1844
tert-butyloxycarbonyl-Gln-Ala-Arg-7-amido-4-methylcoumarin
-
in 100 mM sodium phosphate, 0.01% (v/v) Tween 20, pH 8.5, at 37°C
0.2247
tert-butyloxycarbonyl-Gln-Arg-Arg-7-amido-4-methylcoumarin
-
in 100 mM sodium phosphate, 0.01% (v/v) Tween 20, pH 8.5, at 37°C
0.3699
tert-butyloxycarbonyl-Gln-Gly-Arg-7-amido-4-methylcoumarin
-
in 100 mM sodium phosphate, 0.01% (v/v) Tween 20, pH 8.5, at 37°C
0.1165
tert-butyloxycarbonyl-Leu-Arg-Arg-7-amido-4-methylcoumarin
-
in 100 mM sodium phosphate, 0.01% (v/v) Tween 20, pH 8.5, at 37°C
0.1407
tert-butyloxycarbonyl-Leu-Lys-Arg-7-amido-4-methylcoumarin
-
in 100 mM sodium phosphate, 0.01% (v/v) Tween 20, pH 8.5, at 37°C
0.22 - 0.54
tert-butyloxycarbonyl-Phe-Ser-Arg-4-methylcoumaryl-7-amide
0.2983
tert-butyloxycarbonyl-Phe-Ser-Arg-7-amido-4-methylcoumarin
-
in 100 mM sodium phosphate, 0.01% (v/v) Tween 20, pH 8.5, at 37°C
0.3326
tert-butyloxycarbonyl-Val-Leu-Lys-7-amido-4-methylcoumarin
-
in 100 mM sodium phosphate, 0.01% (v/v) Tween 20, pH 8.5, at 37°C
0.27 - 0.3
tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumaryl-7-amide
0.0238
tert-butyloxycarbonyl-Val-Pro-Arg-7-amido-4-methylcoumarin
-
in 100 mM sodium phosphate, 0.01% (v/v) Tween 20, pH 8.5, at 37°C
0.6897
tosyl-Gly-Pro-Arg-7-amido-4-methylcoumarin
-
in 100 mM sodium phosphate, 0.01% (v/v) Tween 20, pH 8.5, at 37°C