Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.3.2.6: leukotriene-A4 hydrolase

This is an abbreviated version!
For detailed information about leukotriene-A4 hydrolase, go to the full flat file.

Word Map on EC 3.3.2.6

Reaction

leukotriene A4
+
H2O
=
leukotriene B4

Synonyms

EH, epoxide hydrolase, hydrolase, leukotriene A4, leukotriene A(4) hydrolase, leukotriene A4 hydrolase, leukotriene A4 hydrolase/aminopeptidase, leukotriene hydrolase A4, leukotriene-A4 hydrolase, leukotriene-A4-hydrolase, LT A4 hydrolase, LTA-4 hydrolase, LTA4 hydrolase, LTA4-h, LTA4H, More

ECTree

     3 Hydrolases
         3.3 Acting on ether bonds
             3.3.2 Ether hydrolases
                3.3.2.6 leukotriene-A4 hydrolase

Purification

Purification on EC 3.3.2.6 - leukotriene-A4 hydrolase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme monomer from leukocytes
Ni-Sepharose column chromatography, and MonoQ column chromatography
recombinant enzyme
recombinant enzyme, expression in Escherichia coli as a fusion protein with ten additional amino acids at the amino terminus
-
recombinant His6-tagged enzyme by nickel affinity chromatography and gel filtration
using metal affinity chromatography, the His tag is removed by digestion with His6-TEV protease
-
wild-type and mutant enzymes