Application | Comment | Organism |
---|---|---|
medicine | the enzyme catalyses the hydrolysis of leukotriene A4 into the proinflammatory substance leukotriene B4 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-chloro-2,4-dinitrobenzene | weak | Homo sapiens | |
2,3-Butanedione | preincubation with bestatin partially protects enzyme from inactivation | Homo sapiens | |
Acetic anhydride | complete loss of peptidase activity and epoxide hydrolase activity | Homo sapiens | |
bestatin | no influence | Homo sapiens | |
citraconic anhydride | complete loss of peptidase activity and epoxide hydrolase activity | Homo sapiens | |
diamide/glutathione combination | complete loss of peptidase activity and epoxide hydrolase activity | Homo sapiens | |
iodoacetamide | weak | Homo sapiens | |
iodoacetic acid | weak | Homo sapiens | |
methyl methanethiosulfonate | inhibition of epoxide hydrolase activity, stimulation of peptidase activity | Homo sapiens | |
additional information | not diethyl dicarbonate | Homo sapiens | |
N-Acetylimidazole | 75 min, loss of 92% peptidase activity and 78% of epoxide hydrolase activity, both activities are restored by hydroxylamine and bestatin protects | Homo sapiens | |
N-ethylmaleimide | weak | Homo sapiens | |
Phenylglyoxal | preincubation with captopril protects enzyme from inactivation | Homo sapiens | |
Tetranitromethane | preincubation with bestatin prevents the inactivation | Homo sapiens | |
Trinitrobenzenesulfonate | complete loss of peptidase activity and epoxide hydrolase activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.47 | - |
alanine-4-nitroanilide | pH 7.5, 25°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | zinc protein | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
leukotriene A4 + H2O | Homo sapiens | biosynthesis of leukotriene B4 | leukotriene B4 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
leukotriene A4 + H2O = leukotriene B4 | bifunctional enzyme acting as an epoxide hydrolase and also as an aminopeptidase | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
leukotriene A4 + H2O | - |
Homo sapiens | leukotriene B4 | - |
? | |
leukotriene A4 + H2O | biosynthesis of leukotriene B4 | Homo sapiens | leukotriene B4 | - |
? | |
additional information | - |
Homo sapiens | ? | - |
? |