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Literature summary for 3.3.2.6 extracted from

  • Mueller, M.J.; Samuelsson, B.; Haeggstrom, J.Z.
    Chemical modification of leukotriene A4 hydrolase. Indications for essential tyrosyl and arginyl residues at the active site (1995), Biochemistry, 34, 3536-3543.
    View publication on PubMed

Application

Application Comment Organism
medicine the enzyme catalyses the hydrolysis of leukotriene A4 into the proinflammatory substance leukotriene B4 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1-chloro-2,4-dinitrobenzene weak Homo sapiens
2,3-Butanedione preincubation with bestatin partially protects enzyme from inactivation Homo sapiens
Acetic anhydride complete loss of peptidase activity and epoxide hydrolase activity Homo sapiens
bestatin no influence Homo sapiens
citraconic anhydride complete loss of peptidase activity and epoxide hydrolase activity Homo sapiens
diamide/glutathione combination complete loss of peptidase activity and epoxide hydrolase activity Homo sapiens
iodoacetamide weak Homo sapiens
iodoacetic acid weak Homo sapiens
methyl methanethiosulfonate inhibition of epoxide hydrolase activity, stimulation of peptidase activity Homo sapiens
additional information not diethyl dicarbonate Homo sapiens
N-Acetylimidazole 75 min, loss of 92% peptidase activity and 78% of epoxide hydrolase activity, both activities are restored by hydroxylamine and bestatin protects Homo sapiens
N-ethylmaleimide weak Homo sapiens
Phenylglyoxal preincubation with captopril protects enzyme from inactivation Homo sapiens
Tetranitromethane preincubation with bestatin prevents the inactivation Homo sapiens
Trinitrobenzenesulfonate complete loss of peptidase activity and epoxide hydrolase activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.47
-
alanine-4-nitroanilide pH 7.5, 25°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc zinc protein Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
leukotriene A4 + H2O Homo sapiens biosynthesis of leukotriene B4 leukotriene B4
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
leukotriene A4 + H2O = leukotriene B4 bifunctional enzyme acting as an epoxide hydrolase and also as an aminopeptidase Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
leukotriene A4 + H2O
-
Homo sapiens leukotriene B4
-
?
leukotriene A4 + H2O biosynthesis of leukotriene B4 Homo sapiens leukotriene B4
-
?
additional information
-
Homo sapiens ?
-
?